Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QUE41_RS03195 Genome accession   NZ_AP027368
Coordinates   668235..669350 (+) Length   371 a.a.
NCBI ID   WP_286341509.1    Uniprot ID   -
Organism   Ferrimonas sp. YFM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 663235..674350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE41_RS03160 (F0521_05920) rdgB 663633..664229 (-) 597 WP_286341503.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  QUE41_RS03165 (F0521_05930) - 664293..664730 (-) 438 WP_286341504.1 DUF4426 domain-containing protein -
  QUE41_RS03170 (F0521_05940) yggU 664794..665081 (-) 288 WP_286341505.1 DUF167 family protein YggU -
  QUE41_RS03175 (F0521_05950) - 665078..665626 (-) 549 WP_286341506.1 YggT family protein -
  QUE41_RS03180 (F0521_05960) proC 665653..666471 (-) 819 WP_286342898.1 pyrroline-5-carboxylate reductase -
  QUE41_RS03185 (F0521_05970) - 666483..667166 (-) 684 WP_286341507.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE41_RS03190 (F0521_05980) pilT 667195..668226 (+) 1032 WP_286341508.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE41_RS03195 (F0521_05990) pilU 668235..669350 (+) 1116 WP_286341509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE41_RS03200 (F0521_06000) - 669374..670021 (+) 648 WP_286341510.1 TetR/AcrR family transcriptional regulator -
  QUE41_RS03205 (F0521_06010) - 670207..672468 (+) 2262 WP_286341511.1 EAL domain-containing protein -
  QUE41_RS03210 (F0521_06020) - 672888..674183 (+) 1296 WP_286341512.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41114.11 Da        Isoelectric Point: 5.8690

>NTDB_id=100896 QUE41_RS03195 WP_286341509.1 668235..669350(+) (pilU) [Ferrimonas sp. YFM]
MEINQLLAQMVSRQASDLFITADFPPSAKIDGVMTPLGEAPLTPEQSLALVESVMTPDTREQFHLHREANFAYSFDESLG
RFRVSAFWQREQAGMVLRRIQTEIPTPEQLHLPQVMTDVAMAKRGLVIMVGATGTGKSTSLAAMVGHRNAHQAGHILTIE
DPVEFVHKHRKSIVTQREVGLDTESFEAALKSSLRQAPDVILIGEIRSQETMEFALAFAETGHLCMATLHANNANQALDR
IMHLVPKERHRQLLFDLSLNLKAVMAQQLVPCKQGNGRRVAIEVLLNTPRVADLIAKGELHELKGTMAASREQGMQTFDQ
ALFDLYMAEQISYEDALHYADSPNDLRLMIKLKANEGSSAGSLEGVTIELD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=100896 QUE41_RS03195 WP_286341509.1 668235..669350(+) (pilU) [Ferrimonas sp. YFM]
ATGGAGATCAATCAGCTATTGGCTCAGATGGTCAGCCGTCAGGCCAGTGACCTGTTTATCACCGCCGATTTTCCTCCCAG
CGCCAAGATTGACGGGGTGATGACTCCCCTTGGTGAGGCGCCTTTGACCCCTGAGCAGTCGCTGGCCCTGGTGGAGTCGG
TGATGACCCCGGACACCAGGGAGCAGTTTCACCTGCACCGGGAGGCCAATTTCGCTTACAGCTTCGATGAGTCATTGGGG
CGTTTCCGGGTCTCTGCATTCTGGCAGCGGGAGCAGGCGGGTATGGTGCTTCGTCGCATCCAGACCGAGATCCCCACCCC
GGAACAGCTGCATCTGCCCCAGGTGATGACGGACGTGGCCATGGCCAAGCGCGGCCTGGTGATCATGGTGGGGGCGACGG
GAACGGGTAAGTCCACCTCTCTGGCGGCCATGGTGGGACACCGCAATGCCCATCAGGCCGGGCATATCCTTACCATCGAA
GATCCGGTGGAGTTTGTCCATAAGCACCGCAAGAGCATAGTGACCCAGAGGGAGGTGGGGCTGGACACCGAGTCTTTCGA
AGCGGCCCTGAAGAGCTCCCTGCGCCAGGCGCCGGACGTGATCCTCATAGGTGAGATCCGCAGTCAGGAGACCATGGAGT
TTGCCCTGGCGTTTGCCGAAACCGGCCACCTGTGTATGGCGACCCTGCACGCCAACAACGCCAACCAGGCTTTGGACCGC
ATCATGCATCTGGTGCCCAAGGAGAGGCATCGGCAGCTGCTGTTTGACCTGTCATTGAATCTCAAGGCGGTGATGGCCCA
ACAGCTGGTGCCCTGCAAGCAGGGCAATGGTCGGCGGGTGGCCATCGAGGTGCTGCTGAACACCCCCAGGGTGGCGGATC
TCATCGCCAAAGGCGAACTGCATGAGCTGAAGGGAACCATGGCGGCCAGTCGGGAACAGGGGATGCAGACCTTCGATCAG
GCGCTGTTTGACCTCTACATGGCTGAGCAGATCAGCTATGAGGATGCCCTTCACTACGCGGATTCCCCCAATGACCTGAG
GCTGATGATTAAACTCAAGGCCAATGAGGGAAGCTCGGCGGGAAGCCTGGAAGGGGTCACCATTGAACTGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.995

100

0.62
  pilU Pseudomonas stutzeri DSM 10701

61.022

100

0.612
  pilU Acinetobacter baylyi ADP1

59.659

94.879

0.566
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.261

0.396
  pilT Pseudomonas aeruginosa PAK

39.595

93.261

0.369
  pilT Vibrio cholerae strain A1552

42.5

86.253

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.5

86.253

0.367
  pilT Legionella pneumophila strain Lp02

39.881

90.566

0.361
  pilT Legionella pneumophila strain ERS1305867

39.881

90.566

0.361

Multiple sequence alignment