Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABNP46_RS18355 Genome accession   NZ_CP157875
Coordinates   3983700..3984809 (+) Length   369 a.a.
NCBI ID   WP_349919721.1    Uniprot ID   -
Organism   Aeromonas veronii strain Ichip_C_3-7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3978700..3989809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNP46_RS18325 (ABNP46_18305) - 3979544..3979963 (-) 420 WP_349919711.1 DUF4426 domain-containing protein -
  ABNP46_RS18330 (ABNP46_18310) yggU 3979968..3980276 (-) 309 WP_349919713.1 DUF167 family protein YggU -
  ABNP46_RS18335 (ABNP46_18315) - 3980276..3980827 (-) 552 WP_349919714.1 YggT family protein -
  ABNP46_RS18340 (ABNP46_18320) proC 3980853..3981677 (-) 825 WP_349919715.1 pyrroline-5-carboxylate reductase -
  ABNP46_RS18345 (ABNP46_18325) - 3981880..3982599 (-) 720 WP_349919717.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNP46_RS18350 (ABNP46_18330) pilT 3982639..3983673 (+) 1035 WP_349919719.1 type IVa pilus ATPase TapT Machinery gene
  ABNP46_RS18355 (ABNP46_18335) pilU 3983700..3984809 (+) 1110 WP_349919721.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNP46_RS18360 (ABNP46_18340) yaaA 3984843..3985616 (+) 774 WP_349919723.1 peroxide stress protein YaaA -
  ABNP46_RS18365 (ABNP46_18345) srmB 3985798..3987021 (-) 1224 WP_349919724.1 ATP-dependent RNA helicase SrmB -
  ABNP46_RS18370 (ABNP46_18350) - 3987149..3987859 (+) 711 WP_349919726.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ABNP46_RS18375 (ABNP46_18355) brnQ 3988096..3989358 (+) 1263 WP_349922565.1 branched-chain amino acid transport system II carrier protein -
  ABNP46_RS18380 (ABNP46_18360) - 3989431..3989658 (-) 228 WP_349919727.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40890.90 Da        Isoelectric Point: 6.4405

>NTDB_id=1008764 ABNP46_RS18355 WP_349919721.1 3983700..3984809(+) (pilU) [Aeromonas veronii strain Ichip_C_3-7]
MNMDVLLAALVEQKGSDLFITVGAPPTLKVNGRLVPLGEVPLDRQTTLTLVRSSLDAAHLERYQHSREANYAIVREGIGR
FRVSAFWQQDQPGMVLRRIETQIPGFEELKLPPILQDVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHSHDRCLVTQREVGIDTESFDVALKNSMRQAPDVILIGEIRTQETMELAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPNLHGQRRVAAFEILLNTPLITDIIRKGEVHRLKEVMSKSTELGMQTFDQA
LFTLFCGGQIGYSEALAHADSANDLRLQIKLSGRQQLGTGSLDSATLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1008764 ABNP46_RS18355 WP_349919721.1 3983700..3984809(+) (pilU) [Aeromonas veronii strain Ichip_C_3-7]
ATGAACATGGATGTTCTGTTGGCTGCACTGGTTGAACAAAAGGGGTCGGATCTCTTTATCACGGTCGGCGCCCCCCCCAC
CCTCAAGGTCAATGGCCGCCTGGTGCCCCTCGGCGAGGTCCCGCTGGATCGCCAGACCACCCTGACGCTGGTCAGGAGCA
GCCTGGATGCCGCGCACCTCGAACGCTACCAGCATAGCCGGGAGGCCAACTACGCCATCGTCCGCGAGGGGATAGGTCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGATCAGCCGGGCATGGTGCTGCGCCGTATCGAAACCCAGATCCCCGGCTTTGA
GGAGCTGAAGCTGCCCCCTATCCTGCAGGATGTCGCCATGGCCAAGCGCGGTCTGGTGCTGTTCGTGGGGGCCACCGGTG
CCGGCAAGTCCACCACCCAGGCCGCCATGATAGGCTTTCGCAATCAGCACGGTGACGGCCATATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTGCACAGCCACGACCGCTGCCTGGTCACCCAGCGAGAGGTGGGCATAGACACCGAGTCGTTCGATGT
GGCACTCAAGAACTCCATGCGCCAGGCCCCGGACGTGATCCTCATCGGCGAGATCCGCACCCAGGAGACCATGGAGCTGG
CCATCCAGTTCGCCGAGACCGGCCACCTCTGTCTCGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCCCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCGCAGCA
ACTGCTGCCCAACCTGCACGGTCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAAGTCCATCGGCTCAAGGAGGTGATGAGCAAATCCACCGAGCTCGGGATGCAAACCTTCGATCAGGCA
CTGTTCACCCTGTTCTGTGGCGGCCAAATTGGCTACAGTGAGGCCCTCGCCCATGCCGACTCCGCCAACGACCTGAGGCT
GCAGATCAAGCTCTCCGGTCGCCAGCAGCTGGGCACAGGTTCACTGGACAGCGCCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.054

99.729

0.599
  pilU Pseudomonas stutzeri DSM 10701

60.684

95.122

0.577
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.964

91.057

0.382
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366

Multiple sequence alignment