Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABL841_RS23505 Genome accession   NZ_CP157636
Coordinates   4980343..4980801 (+) Length   152 a.a.
NCBI ID   WP_018903501.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 4MFCol3.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4975343..4985801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL841_RS23485 (ABL841_23455) - 4975508..4976308 (+) 801 WP_018903497.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ABL841_RS23490 (ABL841_23460) - 4976333..4976989 (+) 657 WP_018903498.1 FHA domain-containing protein -
  ABL841_RS23495 (ABL841_23465) - 4977026..4979302 (+) 2277 WP_018903499.1 CHASE2 domain-containing protein -
  ABL841_RS23500 (ABL841_23470) - 4979335..4980138 (+) 804 WP_018903500.1 3',5'-cyclic-nucleotide phosphodiesterase -
  ABL841_RS23505 (ABL841_23475) pilA 4980343..4980801 (+) 459 WP_018903501.1 pilin Machinery gene
  ABL841_RS23510 (ABL841_23480) - 4980872..4982785 (+) 1914 WP_155247792.1 hypothetical protein -
  ABL841_RS23515 (ABL841_23485) - 4982775..4983212 (+) 438 WP_080642487.1 GtrA family protein -
  ABL841_RS23520 (ABL841_23490) - 4983223..4984848 (+) 1626 WP_038106687.1 NAD(P)/FAD-dependent oxidoreductase -
  ABL841_RS23525 (ABL841_23495) - 4984967..4985452 (+) 486 WP_018903505.1 hypothetical protein -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15671.98 Da        Isoelectric Point: 9.6044

>NTDB_id=1007916 ABL841_RS23505 WP_018903501.1 4980343..4980801(+) (pilA) [Variovorax paradoxus strain 4MFCol3.1]
MNVRKIARRAQAGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVSEAMVLASAAKTLVGENAANAASDLTLGFDSASV
SSTKNVSSMSIDSAGAITVITTAAAGSVTLKLTPTSGGNVLAANQTPSGQIQWKCTTDSSKFKYVPSECRNT

Nucleotide


Download         Length: 459 bp        

>NTDB_id=1007916 ABL841_RS23505 WP_018903501.1 4980343..4980801(+) (pilA) [Variovorax paradoxus strain 4MFCol3.1]
ATGAACGTTCGCAAGATCGCACGCCGCGCGCAGGCCGGCTTTACCCTTATCGAATTGATGATCGTTGTGGCGATCATCGG
CATCTTGGCTGCAGTGGCTCTGCCGGCTTATCAGGATTACACGGTACGCGCACGCGTCTCTGAAGCTATGGTGCTTGCAT
CGGCAGCTAAAACGCTGGTTGGCGAAAACGCTGCCAACGCGGCATCTGACCTTACGCTGGGCTTTGACTCGGCTTCGGTG
AGCTCGACGAAAAACGTTTCATCGATGAGCATCGATTCGGCCGGTGCTATCACGGTCATCACGACGGCTGCGGCAGGTTC
TGTGACGTTGAAACTCACGCCTACTTCGGGTGGCAACGTACTTGCGGCGAATCAGACTCCATCTGGTCAGATTCAGTGGA
AATGCACCACCGACTCGAGCAAGTTCAAGTACGTTCCAAGCGAGTGCCGTAACACCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.19

100

0.566
  comP Acinetobacter baylyi ADP1

52.027

97.368

0.507
  pilA2 Legionella pneumophila str. Paris

50.694

94.737

0.48
  pilA2 Legionella pneumophila strain ERS1305867

50.694

94.737

0.48
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.968

100

0.467
  pilE Neisseria gonorrhoeae strain FA1090

39.13

100

0.414
  pilA/pilA1 Eikenella corrodens VA1

38.994

100

0.408
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.735

99.342

0.395
  pilE Neisseria gonorrhoeae MS11

34.969

100

0.375
  pilA Acinetobacter baumannii strain A118

37.838

97.368

0.368

Multiple sequence alignment