Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAGD24_RS14040 Genome accession   NZ_CP156894
Coordinates   3097734..3099449 (+) Length   571 a.a.
NCBI ID   WP_085679891.1    Uniprot ID   -
Organism   Marinobacter sp. M5B strain M5B10.3.1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3092734..3104449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGD24_RS14015 cysQ 3092824..3093591 (-) 768 WP_379865442.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  AAGD24_RS14020 - 3093723..3094644 (+) 922 Protein_2743 MBL fold metallo-hydrolase RNA specificity domain-containing protein -
  AAGD24_RS14025 - 3094703..3095146 (-) 444 WP_379865443.1 pilin -
  AAGD24_RS14030 - 3095206..3096723 (-) 1518 WP_379865445.1 O-antigen ligase family protein -
  AAGD24_RS14035 - 3096846..3097334 (-) 489 WP_379867355.1 pilin -
  AAGD24_RS14040 pilB 3097734..3099449 (+) 1716 WP_085679891.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAGD24_RS14045 pilC 3099452..3100672 (+) 1221 WP_126812063.1 type II secretion system F family protein Machinery gene
  AAGD24_RS14050 pilD 3100758..3101633 (+) 876 WP_379865447.1 prepilin peptidase Machinery gene
  AAGD24_RS14055 coaE 3101634..3102236 (+) 603 WP_379865449.1 dephospho-CoA kinase -
  AAGD24_RS14060 tsaA 3102242..3102988 (+) 747 WP_036209925.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62790.74 Da        Isoelectric Point: 4.9580

>NTDB_id=1003083 AAGD24_RS14040 WP_085679891.1 3097734..3099449(+) (pilB) [Marinobacter sp. M5B strain M5B10.3.1]
MSTNKSNVTLTGLARRFVDDGLIDESTAKDAFLQASHNRIPLITYLVQNNLANSGGLAFSAAMEFGVSVLDLNAFMPEMM
PDKVVDEKLIRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLREAIDKYLESHDTSMGDLDDA
DLEGVETEGGDQEDDAVAATSEVDDAPIVKYVNKMLLDAIRGGASDVHFEPYEKTYRVRYRTDGILKEMSRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEEQKQLYLDALE
QPQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDIIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPSFNIATSVSLIIAQRLGRRLCNSCRQPADIPKDVLLAE
GFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIKIAKQAQAEGFPNLRQSALKKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1003083 AAGD24_RS14040 WP_085679891.1 3097734..3099449(+) (pilB) [Marinobacter sp. M5B strain M5B10.3.1]
ATGTCTACCAATAAAAGCAATGTAACGCTTACGGGCCTGGCGAGACGCTTCGTCGATGACGGGCTTATTGATGAATCCAC
CGCGAAAGATGCCTTTCTTCAAGCATCCCACAACCGCATTCCACTCATTACCTACCTGGTACAGAACAACCTGGCGAACA
GCGGAGGGCTCGCCTTCTCCGCTGCGATGGAGTTCGGTGTTTCGGTTCTCGATCTCAACGCCTTTATGCCGGAGATGATG
CCCGATAAGGTCGTAGACGAAAAACTGATACGAAAACACAATGCCCTGCCGCTCTATAAACGTGGGAACCGGCTGTTTAT
TGCGGTCTCCGACCCGACCAACATCCAGGCGTTGGACGAAATCAAGTTTAATACCGGCCTGAGTACAGACGCCATCCTGG
TTGACGACGCCAAGCTCCGGGAAGCCATCGACAAGTATCTTGAGTCCCACGATACGTCGATGGGTGATCTGGATGACGCC
GACCTTGAAGGCGTTGAAACGGAAGGCGGCGACCAGGAAGACGATGCCGTTGCCGCGACGAGCGAAGTAGACGATGCCCC
CATCGTGAAGTACGTGAACAAGATGCTTCTTGACGCCATTCGCGGGGGGGCCTCGGACGTTCACTTTGAACCCTATGAGA
AAACCTATCGGGTTCGATACCGCACCGACGGCATTCTGAAAGAAATGTCGCGGCCCTCCATCAAGCTGGCCCCGAAGATT
TCTGCCCGGGTAAAAATCATGGCGCAGCTGGATATCTCGGAGCGCCGGGTCCCCCAGGACGGCCGGATCAAGATGAAGCT
GTCCAAGACCAAGGCCATCGACTTCCGGGTCAATACTCTGCCGACACTGTGGGGAGAAAAGATCGTTCTGCGAATTCTGG
ACCCAAGTCAGGCGAAGCTCGGCATCGATGTCCTGGGTTATGAGGAAGAACAGAAACAACTGTACCTGGACGCCCTTGAG
CAGCCACAGGGAATGATACTGGTGACGGGGCCCACCGGCTCAGGCAAGACGGTTTCCCTTTATACCGGCCTGAACATACT
GAACACACCAGAAAGGAACATCTCCACAGCCGAAGACCCTGCGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCA
ATACCAGGGTGGGCCTCGGCTTTGCGGAGGCATTGAGAGCCTTCCTCCGCCAGGACCCGGACATCATCATGGTGGGTGAG
ATTCGCGACCTGGAAACGGCCAACATTGCTATCAAAGCGGCACAAACAGGGCACCTGGTGCTCTCGACCCTGCACACCAA
CAGTGCTGCGGAAACGCTCACGCGCATGATGAACATGGGCGTCCCCTCCTTCAACATCGCCACATCGGTGAGCCTGATCA
TTGCCCAGCGACTGGGCCGTCGGCTTTGTAACTCTTGTAGACAACCTGCCGACATCCCCAAAGACGTGCTTTTAGCGGAG
GGGTTCACACAGGAACAAATTGATACAGGGTTCACGTTGTACCGTCCCAAGGGCTGTGATAAATGCAATGGTGGATATAA
AGGCCGCGTCGGGATTTACGAGGTGGTCAAGATTACCGACGAGCTGGCGAACATGATTATGGAAGAAGCCAGTTCGATTA
AAATTGCAAAGCAGGCACAGGCCGAAGGCTTCCCGAACTTGCGGCAGTCTGCCCTCAAAAAAGTCATTGAGGGCGTGACC
AGTCTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(21-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.894

100

0.571
  pilB Acinetobacter baylyi ADP1

56.643

100

0.567
  pilB Legionella pneumophila strain ERS1305867

55.124

99.124

0.546
  pilB Vibrio parahaemolyticus RIMD 2210633

52.065

97.548

0.508
  pilB Vibrio campbellii strain DS40M4

50.709

98.774

0.501
  pilB Vibrio cholerae strain A1552

53.846

91.068

0.49
  pilF Neisseria gonorrhoeae MS11

47.527

99.124

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.304

92.119

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

40.734

90.718

0.37

Multiple sequence alignment