Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AAGD24_RS14050 Genome accession   NZ_CP156894
Coordinates   3100758..3101633 (+) Length   291 a.a.
NCBI ID   WP_379865447.1    Uniprot ID   -
Organism   Marinobacter sp. M5B strain M5B10.3.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3095758..3106633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGD24_RS14035 - 3096846..3097334 (-) 489 WP_379867355.1 pilin -
  AAGD24_RS14040 pilB 3097734..3099449 (+) 1716 WP_085679891.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAGD24_RS14045 pilC 3099452..3100672 (+) 1221 WP_126812063.1 type II secretion system F family protein Machinery gene
  AAGD24_RS14050 pilD 3100758..3101633 (+) 876 WP_379865447.1 prepilin peptidase Machinery gene
  AAGD24_RS14055 coaE 3101634..3102236 (+) 603 WP_379865449.1 dephospho-CoA kinase -
  AAGD24_RS14060 tsaA 3102242..3102988 (+) 747 WP_036209925.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  AAGD24_RS14065 - 3103021..3105381 (+) 2361 WP_227662347.1 response regulator -
  AAGD24_RS14070 yacG 3105402..3105590 (+) 189 WP_036209919.1 DNA gyrase inhibitor YacG -
  AAGD24_RS14075 - 3105692..3106246 (+) 555 WP_036209917.1 YfaZ family outer membrane protein -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32128.15 Da        Isoelectric Point: 4.9961

>NTDB_id=1003085 AAGD24_RS14050 WP_379865447.1 3100758..3101633(+) (pilD) [Marinobacter sp. M5B strain M5B10.3.1]
MISVDTFLATPWLLYTAVIFFSLCIGSFLNVVILRLPKMMQQDWRCQCEEFLELSDEQRKNDEPITLSKPASTCPSCGHS
IRAWENVPVISYLVLGGKCASCKTGISPRYPMIEAITALFSVVTILVMGPSEAALWSLLLVWVLIALTVIDFDTQLLPDS
MTLPLMWLGLILNYFGVLTEFSSAFWGAVAGYLSLWSVYWLFKLVTGKEGMGHGDFKLLAALGAWLGWELLPAVILLSSL
VGAVVGISLMVFRKHGREVPIPFGPYLAAAGLLCLWFGAEIQAAWFTYLGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=1003085 AAGD24_RS14050 WP_379865447.1 3100758..3101633(+) (pilD) [Marinobacter sp. M5B strain M5B10.3.1]
ATGATTTCCGTTGATACGTTCCTGGCCACGCCCTGGCTTCTCTATACCGCAGTCATTTTCTTCTCCCTCTGCATCGGTAG
CTTCCTGAATGTGGTGATCCTTCGCCTGCCGAAGATGATGCAGCAGGACTGGCGCTGCCAATGCGAAGAGTTCCTGGAGC
TATCAGACGAGCAACGCAAGAACGATGAGCCGATTACGCTCTCCAAACCTGCCTCCACCTGCCCTTCTTGCGGCCACAGC
ATTCGCGCCTGGGAAAACGTCCCGGTCATCAGCTACCTGGTTTTGGGCGGCAAGTGCGCGTCCTGCAAAACGGGCATTTC
ACCGCGATACCCAATGATTGAGGCAATTACCGCGCTGTTTTCGGTGGTGACCATTCTGGTCATGGGGCCATCTGAGGCTG
CATTATGGTCTCTGCTGTTGGTGTGGGTATTGATCGCACTGACCGTGATTGATTTCGACACCCAGTTGTTGCCAGACAGC
ATGACGCTGCCCCTCATGTGGCTCGGGCTGATACTGAATTATTTCGGTGTGCTGACGGAATTCAGCAGCGCGTTCTGGGG
TGCGGTAGCGGGCTATCTCTCCCTCTGGTCGGTGTACTGGTTGTTCAAGTTGGTAACCGGCAAGGAGGGCATGGGCCACG
GCGATTTCAAGTTATTGGCGGCGCTGGGCGCTTGGTTAGGCTGGGAATTGCTGCCGGCGGTGATTTTGTTGTCGTCTTTG
GTCGGTGCCGTTGTGGGGATTAGTTTGATGGTGTTCCGCAAGCACGGCCGCGAAGTGCCAATTCCGTTCGGGCCCTATTT
GGCGGCAGCGGGTTTGCTCTGCCTTTGGTTTGGGGCAGAAATTCAGGCAGCCTGGTTTACCTATCTGGGGGTTTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

55.235

95.189

0.526
  pilD Acinetobacter nosocomialis M2

54.874

95.189

0.522
  pilD Vibrio campbellii strain DS40M4

51.399

98.282

0.505
  pilD Vibrio cholerae strain A1552

50.35

98.282

0.495
  pilD Neisseria gonorrhoeae MS11

51.984

86.598

0.45

Multiple sequence alignment