Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   EL254_RS01125 Genome accession   NZ_LR134512
Coordinates   188904..189359 (+) Length   151 a.a.
NCBI ID   WP_000793378.1    Uniprot ID   Q8E234
Organism   Streptococcus agalactiae strain NCTC13949     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 183904..194359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL254_RS01105 (NCTC13949_00221) - 186461..186826 (+) 366 WP_000285373.1 DUF1033 family protein -
  EL254_RS01115 (NCTC13949_00222) comGA 186999..187970 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  EL254_RS01120 (NCTC13949_00223) comGB 187864..188907 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL254_RS01125 (NCTC13949_00224) comGF 188904..189359 (+) 456 WP_000793378.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL254_RS01130 (NCTC13949_00225) comGG 189337..189708 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG Machinery gene
  EL254_RS01135 (NCTC13949_00226) comGH 189823..190797 (+) 975 WP_001008574.1 class I SAM-dependent methyltransferase Machinery gene
  EL254_RS01140 (NCTC13949_00227) - 190829..192022 (+) 1194 WP_000047534.1 acetate kinase -
  EL254_RS01145 (NCTC13949_00228) - 192174..192380 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  EL254_RS01150 - 192439..192576 (+) 138 WP_001865900.1 hypothetical protein -
  EL254_RS01155 (NCTC13949_00229) - 192617..193072 (+) 456 WP_000905673.1 hypothetical protein -
  EL254_RS01160 (NCTC13949_00230) - 193141..193806 (+) 666 WP_000008113.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 17509.47 Da        Isoelectric Point: 9.7378

>NTDB_id=1003085 EL254_RS01125 WP_000793378.1 188904..189359(+) (comGF) [Streptococcus agalactiae strain NCTC13949]
MKNLLLKCKDKKVKAFTLLECLVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNK
LYLRKQDKIVTFGKSNKDDFRKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET

Nucleotide


Download         Length: 456 bp        

>NTDB_id=1003085 EL254_RS01125 WP_000793378.1 188904..189359(+) (comGF) [Streptococcus agalactiae strain NCTC13949]
ATGAAAAATTTATTGTTAAAATGTAAGGATAAGAAGGTTAAAGCATTTACACTTTTAGAATGTTTGGTAGCATTGGTTAC
AATCACAGGAGCTTTACTAGTTTATCAAGGACTGACAAAATTGTTGGCTCAACAGATAGTAGTGATGTCTTCTTCCAGTC
AGTCTGAATGGGTGTTATTAACTCAGCAACTAAATGCAGAATTTGAAGGCGCTCATCTGGAATATTTAAGACAGAACAAA
CTTTATTTACGTAAGCAAGATAAGATTGTAACCTTTGGCAAATCTAATAAAGATGATTTCCGTAAGACAGGTTATGATGG
TCGAGGTTATCAACCAATGGTTTATGGGTTAGACAATTGTCAAATGAGTCAGACCAAAAGTATGGTAAAACTTGTTTTTT
ATTTTAAGGACGGGTTAAAAAGGACATTTTACTATGATTTTAAAGAAGAAACTTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E234

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Streptococcus mutans UA159

48.571

92.715

0.45
  comGF Streptococcus mutans UA140

48.571

92.715

0.45
  comGF/cglF Streptococcus pneumoniae Rx1

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae D39

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae R6

42

99.338

0.417
  comGF/cglF Streptococcus pneumoniae TIGR4

42

99.338

0.417
  comGF/cglF Streptococcus mitis SK321

44.203

91.391

0.404
  comGF/cglF Streptococcus mitis NCTC 12261

43.478

91.391

0.397
  comGF Lactococcus lactis subsp. cremoris KW2

40.58

91.391

0.371

Multiple sequence alignment