Detailed information    

insolico Bioinformatically predicted

Overview


Name   comP   Type   Machinery gene
Locus tag   ABFU14_RS05330 Genome accession   NZ_CP155961
Coordinates   1267549..1267971 (+) Length   140 a.a.
NCBI ID   WP_323475831.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain NBC271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1265960..1278208 1267549..1267971 within 0


Gene organization within MGE regions


Location: 1265960..1278208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU14_RS05325 (ABFU14_05325) pilC 1265960..1267222 (-) 1263 WP_407471758.1 type II secretion system F family protein Machinery gene
  ABFU14_RS05330 (ABFU14_05330) comP 1267549..1267971 (+) 423 WP_323475831.1 pilin Machinery gene
  ABFU14_RS05335 (ABFU14_05335) pilA2 1268140..1268517 (+) 378 WP_407471905.1 pilin Machinery gene
  ABFU14_RS05340 (ABFU14_05340) pilB 1268690..1270399 (+) 1710 WP_323475836.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU14_RS05345 (ABFU14_05345) - 1270582..1271373 (-) 792 WP_407471759.1 zeta toxin family protein -
  ABFU14_RS05350 (ABFU14_05350) - 1271318..1271674 (-) 357 WP_407471760.1 hypothetical protein -
  ABFU14_RS05355 (ABFU14_05355) pilR 1271872..1273266 (-) 1395 WP_043921909.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU14_RS05360 (ABFU14_05360) - 1273475..1275085 (-) 1611 WP_115006667.1 PAS domain-containing sensor histidine kinase -
  ABFU14_RS05365 (ABFU14_05365) sucC 1275319..1276488 (+) 1170 WP_011038209.1 ADP-forming succinate--CoA ligase subunit beta -
  ABFU14_RS05370 (ABFU14_05370) sucD 1276513..1277388 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  ABFU14_RS05375 (ABFU14_05375) - 1277492..1277902 (+) 411 WP_029628876.1 CopG family ribbon-helix-helix protein -
  ABFU14_RS05380 (ABFU14_05380) - 1277906..1278208 (+) 303 WP_011038207.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence


Protein


Download         Length: 140 a.a.        Molecular weight: 13878.97 Da        Isoelectric Point: 8.1053

>NTDB_id=1000317 ABFU14_RS05330 WP_323475831.1 1267549..1267971(+) (comP) [Xanthomonas campestris pv. raphani strain NBC271]
MNKQQGFTLIELMIVVAIIAILAAIALPAYQDYTIRARVSEAAVLASGAKTTVAENIANNGGTIAAGACLGVDTTAPATT
NVASVTCGANSGAIAITTTAKAGAVTLTFTPQPAAGIAGTKWTCVATGGKAAYYPAECRS

Nucleotide


Download         Length: 423 bp        

>NTDB_id=1000317 ABFU14_RS05330 WP_323475831.1 1267549..1267971(+) (comP) [Xanthomonas campestris pv. raphani strain NBC271]
ATGAACAAGCAACAAGGTTTTACCCTTATTGAATTGATGATCGTTGTGGCGATCATTGCTATTCTGGCAGCTATCGCTTT
GCCTGCATATCAAGACTATACAATCCGTGCACGTGTCTCTGAGGCTGCCGTGCTTGCTTCGGGTGCTAAAACTACAGTTG
CTGAGAACATCGCAAATAACGGCGGAACAATAGCTGCCGGCGCATGTCTTGGCGTCGATACCACCGCGCCGGCAACCACA
AATGTCGCTAGCGTTACCTGTGGCGCCAATAGCGGAGCGATTGCAATTACCACAACTGCTAAGGCCGGTGCAGTTACCTT
GACCTTTACTCCGCAGCCTGCTGCCGGTATTGCTGGTACTAAGTGGACCTGTGTTGCGACCGGCGGTAAGGCAGCTTACT
ATCCGGCTGAATGCCGTAGTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Acinetobacter baylyi ADP1

53.691

100

0.571

  pilA2 Legionella pneumophila str. Paris

56.115

99.286

0.557

  pilA2 Legionella pneumophila strain ERS1305867

55.396

99.286

0.55

  pilA Ralstonia pseudosolanacearum GMI1000

45.238

100

0.543

  pilA/pilA1 Eikenella corrodens VA1

40.789

100

0.443

  pilA Vibrio parahaemolyticus RIMD 2210633

40.441

97.143

0.393

  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.486

100

0.386

  pilA Acinetobacter baumannii strain A118

38.806

95.714

0.371


Multiple sequence alignment