Detailed information NTG1000318

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: ABFU14_RS05335
• Genome accession: NZ_CP155961
• Coordinates: 1268140..1268517 (+)
• Length: 125 a.a.
• NCBI ID: WP_407471905.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. raphani strain NBC271
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1265960..1278208	1268140..1268517	within	0

Gene organization within MGE regions

Location: 1265960..1278208

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABFU14_RS05325 (ABFU14_05325)	pilC	1265960..1267222 (-)	1263	WP_407471758.1	type II secretion system F family protein	Machinery gene
ABFU14_RS05330 (ABFU14_05330)	comP	1267549..1267971 (+)	423	WP_323475831.1	pilin	Machinery gene
ABFU14_RS05335 (ABFU14_05335)	pilA2	1268140..1268517 (+)	378	WP_407471905.1	pilin	Machinery gene
ABFU14_RS05340 (ABFU14_05340)	pilB	1268690..1270399 (+)	1710	WP_323475836.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ABFU14_RS05345 (ABFU14_05345)	-	1270582..1271373 (-)	792	WP_407471759.1	zeta toxin family protein	-
ABFU14_RS05350 (ABFU14_05350)	-	1271318..1271674 (-)	357	WP_407471760.1	hypothetical protein	-
ABFU14_RS05355 (ABFU14_05355)	pilR	1271872..1273266 (-)	1395	WP_043921909.1	sigma-54 dependent transcriptional regulator	Regulator
ABFU14_RS05360 (ABFU14_05360)	-	1273475..1275085 (-)	1611	WP_115006667.1	PAS domain-containing sensor histidine kinase	-
ABFU14_RS05365 (ABFU14_05365)	sucC	1275319..1276488 (+)	1170	WP_011038209.1	ADP-forming succinate--CoA ligase subunit beta	-
ABFU14_RS05370 (ABFU14_05370)	sucD	1276513..1277388 (+)	876	WP_011038208.1	succinate--CoA ligase subunit alpha	-
ABFU14_RS05375 (ABFU14_05375)	-	1277492..1277902 (+)	411	WP_029628876.1	CopG family ribbon-helix-helix protein	-
ABFU14_RS05380 (ABFU14_05380)	-	1277906..1278208 (+)	303	WP_011038207.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Sequence

Protein

• Length: 125 a.a.
• Molecular weight: 12919.94 Da
• Isoelectric Point: 8.1213

>NTDB_id=1000318 ABFU14_RS05335 WP_407471905.1 1268140..1268517(+) (pilA2) [Xanthomonas campestris pv. raphani strain NBC271]
MIVVAIIAITASIALPAYQDYVIRARVAEAVVLLSGAKALVTENINNENLLAGSACNGVSGAAPATANVSNFTCSGNGVL
TVQTTAKAGAVTITLLPTYVQEEPVRWRCVRTGGAIKHVPPECRN

Nucleotide

• Length: 378 bp

>NTDB_id=1000318 ABFU14_RS05335 WP_407471905.1 1268140..1268517(+) (pilA2) [Xanthomonas campestris pv. raphani strain NBC271]
ATGATAGTAGTTGCGATCATAGCAATTACTGCATCAATTGCCTTGCCCGCCTATCAGGACTATGTAATACGCGCTCGTGT
AGCTGAGGCTGTCGTTTTGCTCAGTGGCGCAAAAGCGCTGGTAACTGAGAACATAAACAATGAGAATTTATTGGCGGGGT
CTGCTTGTAATGGTGTCTCCGGTGCTGCTCCAGCTACAGCCAATGTAAGTAATTTTACATGTAGTGGTAATGGAGTTTTG
ACCGTTCAAACTACAGCTAAGGCCGGGGCTGTTACCATTACATTATTGCCTACATATGTGCAAGAGGAGCCCGTAAGGTG
GAGGTGCGTGCGTACAGGAGGTGCAATAAAACACGTGCCTCCTGAGTGCAGAAATTAG

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (24-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	48.031	100	0.488
pilA2	Legionella pneumophila strain ERS1305867	48.031	100	0.488
pilA	Ralstonia pseudosolanacearum GMI1000	38.961	100	0.48
pilE	Neisseria gonorrhoeae MS11	33.758	100	0.424
comP	Acinetobacter baylyi ADP1	42.5	96	0.408
pilE	Neisseria gonorrhoeae strain FA1090	32.877	100	0.384