Detailed information NTG1000319

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ABFU14_RS05340
• Genome accession: NZ_CP155961
• Coordinates: 1268690..1270399 (+)
• Length: 569 a.a.
• NCBI ID: WP_323475836.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. raphani strain NBC271
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1265960..1278208	1268690..1270399	within	0

Gene organization within MGE regions

Location: 1265960..1278208

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABFU14_RS05325 (ABFU14_05325)	pilC	1265960..1267222 (-)	1263	WP_407471758.1	type II secretion system F family protein	Machinery gene
ABFU14_RS05330 (ABFU14_05330)	comP	1267549..1267971 (+)	423	WP_323475831.1	pilin	Machinery gene
ABFU14_RS05335 (ABFU14_05335)	pilA2	1268140..1268517 (+)	378	WP_407471905.1	pilin	Machinery gene
ABFU14_RS05340 (ABFU14_05340)	pilB	1268690..1270399 (+)	1710	WP_323475836.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ABFU14_RS05345 (ABFU14_05345)	-	1270582..1271373 (-)	792	WP_407471759.1	zeta toxin family protein	-
ABFU14_RS05350 (ABFU14_05350)	-	1271318..1271674 (-)	357	WP_407471760.1	hypothetical protein	-
ABFU14_RS05355 (ABFU14_05355)	pilR	1271872..1273266 (-)	1395	WP_043921909.1	sigma-54 dependent transcriptional regulator	Regulator
ABFU14_RS05360 (ABFU14_05360)	-	1273475..1275085 (-)	1611	WP_115006667.1	PAS domain-containing sensor histidine kinase	-
ABFU14_RS05365 (ABFU14_05365)	sucC	1275319..1276488 (+)	1170	WP_011038209.1	ADP-forming succinate--CoA ligase subunit beta	-
ABFU14_RS05370 (ABFU14_05370)	sucD	1276513..1277388 (+)	876	WP_011038208.1	succinate--CoA ligase subunit alpha	-
ABFU14_RS05375 (ABFU14_05375)	-	1277492..1277902 (+)	411	WP_029628876.1	CopG family ribbon-helix-helix protein	-
ABFU14_RS05380 (ABFU14_05380)	-	1277906..1278208 (+)	303	WP_011038207.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Sequence

Protein

• Length: 569 a.a.
• Molecular weight: 61883.93 Da
• Isoelectric Point: 5.6021

>NTDB_id=1000319 ABFU14_RS05340 WP_323475836.1 1268690..1270399(+) (pilB) [Xanthomonas campestris pv. raphani strain NBC271]
MGITGIARRLVQDGVLDEVNARAAMDQAASAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDASQNAVKLVSE
ELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASSAALGSSLGDDDDEMGDL
DVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLSQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKLFLDAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKATLPEHALLAEGF
TQQQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALVKAAHGVTSL
AEINRVTKD

Nucleotide

• Length: 1710 bp

>NTDB_id=1000319 ABFU14_RS05340 WP_323475836.1 1268690..1270399(+) (pilB) [Xanthomonas campestris pv. raphani strain NBC271]
GTGGGAATTACGGGGATAGCGCGACGGTTAGTTCAAGACGGCGTGCTTGACGAAGTTAATGCTCGGGCAGCAATGGATCA
GGCTGCCTCGGCGAAAGTTCCATTACCACAGTGGTTTGCTGAGAAGAAGCTAGTTTCAGCCGCTCAGCTAGCTGCAGCCA
ATGCAGTCGAGTTCGGCATGCCGCTGATGGACGTGTCGGTCTTCGACGCCAGCCAAAACGCAGTCAAGCTGGTCAGTGAG
GAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGTAAGCAACCCGACCCA
GACCAGGGCACTGGACGATATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGGTTGATGAGGACCAGATCCGTC
GCACCTTGGAGCAGTGGCAGGCGAGCAGTGCGGCGCTCGGCTCTTCGCTCGGTGACGACGACGACGAAATGGGGGATCTG
GACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCCATTTCGAGCCCTATGAAGACGACT
ATCGGGTGCGGTTGCGCATCGATGGCCTGCTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTGAGTCAGCGCATCGCGGCG
CGCTTGAAGGTTATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGTATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTGAGCACCCTGCCGACCTTGTTCGGCGAGAAAGTGGTGCTGCGTATTCTGGACGGCA
GCGCGGCCAAGCTGGGTATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTGTTCCTGGATGCGATCCATAAGCCG
TACGGCATGGTGCTGGTGACCGGTCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATACGGCGCTGGGCATCTTGAACGA
CGAGACGCGCAATATCTCTACGGCCGAGGATCCGGTGGAAATCCGCCTGCCTGGCGTCAACCAGGTGCAGCAGAACAACA
AGCGCGGCATGACCTTCGCTGCGGCCTTGCGCTCGTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATTCGC
GACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACAGGCCACATGGTGCTGTCGACGCTGCATACCAACGATGC
GCCGCAGACCATCGCGCGCTTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCTTGGTCATCGCGC
AGCGCCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGGCCACCTTGCCCGAGCATGCGTTGCTGGCCGAAGGGTTC
ACGCAACAGCAGATCGCTGCAGGTATCGAGCTGTATGAGGCGGTGGGCTGCGACGAGTGCACGGAAGGCTACAAGGGCCG
TACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAAGGCGGCAATGCGATGCAGATCG
CCGAGGCGGCGCAGAAGATCGGCATCCGCGATCTACGCCAGTCGGCGCTGGTCAAGGCGGCGCATGGTGTGACGAGCCTG
GCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (192-459)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (40-144)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.162	99.824	0.561
pilB	Acinetobacter baylyi ADP1	55.282	99.824	0.552
pilB	Legionella pneumophila strain ERS1305867	51.845	100	0.518
pilB	Vibrio cholerae strain A1552	49.12	99.824	0.49
pilF	Neisseria gonorrhoeae MS11	48.944	99.824	0.489
pilB	Vibrio campbellii strain DS40M4	45.518	100	0.455
pilB	Vibrio parahaemolyticus RIMD 2210633	45.263	100	0.453
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.509	92.97	0.367
pilF	Thermus thermophilus HB27	39.808	91.388	0.364