Detailed information of regulator


Summary

Regulator ID REG0331 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-HK
KEGG pathway (determined by BlastKOALA) Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Pseudomonas aeruginosa (map02025); Biofilm formation - Escherichia coli (map02026)
Related T6SS T6SS01266 insolico (Type i1); T6SS01267 insolico (Type i3)
Strain Pseudomonas chlororaphis subsp. aureofaciens 30-84
Replicon chromosome
Sequence Protein sequence (214 a.a.); Nucleotide sequence (642 bp)
Description -
Reference
External database links

Locus tag (Gene) Pchl3084_3491 (gacA)
Coordinates (Strand) 4134794..4135435 (-)
NCBI ID 496335231
RefSeq NZ_CM001559
Uniprot ID UPI00000B86EE
KEGG ID -
KEGG pathway (determined by BlastKOALA) map02020; map05111; map02025; map02026
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
Pchl3084_5419 (rsmY)promotor ↓ GTCATGTAAGCATTCTCTTACATGACGTGCTGCTAATCACCCATATCGGCAGATTGGATCCAGAGCTAATCTACTTCAC Upstream activation sequence(UAS) was indicated in red
Pchl3084_1190 (rsmZ)promotor ↓ AGCATGTAAGTAAAGGCTGACTCTTTCGTAAGTG
Pchl3084_1189 (rpoS)transcript ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Response_reg PF00072.26 1.7e-27 4..116
GerE PF00196.21 1.1e-12 148..203
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-213 MIRVLVVDDHDLVRTGITRMLADIDGLQVVGQAESGEESLIKARELKPDVVLMDVKMPGIGGLEATRKLLRSHPDIKVVAVTVCEEDPFPTRLLQAGAAGYLTKGAGLNEMVQAIRLVFAGQRYISPQIAQQLVFKSFQPSSDSPFDALSEREIQIALMIVGCQKVQIISDKLCLSPKTVNTYRYRIFEKLSISSDVELTLLAVRHGMVDASA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9958 - -


  Protein sequence of regulator: 214 a.a.    .

>REG0331 NZ_CM001559:c4135435-4134794 [Pseudomonas chlororaphis subsp. aureofaciens 30-84]
MIRVLVVDDHDLVRTGITRMLADIDGLQVVGQAESGEESLIKARELKPDVVLMDVKMPGIGGLEATRKLLRSHPDIKVVA
VTVCEEDPFPTRLLQAGAAGYLTKGAGLNEMVQAIRLVFAGQRYISPQIAQQLVFKSFQPSSDSPFDALSEREIQIALMI
VGCQKVQIISDKLCLSPKTVNTYRYRIFEKLSISSDVELTLLAVRHGMVDASA*

  Nucleotide sequence of regulator: 642 bp    .

>REG0331 NZ_CM001559:c4135435-4134794 [Pseudomonas chlororaphis subsp. aureofaciens 30-84]
TTGATTAGGGTGCTAGTAGTCGATGACCATGATCTCGTTCGTACAGGTATTACACGAATGCTGGCTGACATCGATGGCCT
GCAAGTGGTCGGTCAGGCCGAGTCAGGGGAGGAATCCCTGATCAAGGCCCGGGAATTGAAACCCGATGTGGTCCTCATGG
ACGTCAAGATGCCCGGGATCGGCGGTCTTGAAGCCACGCGCAAGTTATTGCGCAGTCACCCGGATATCAAAGTCGTGGCC
GTCACCGTGTGTGAAGAAGATCCGTTCCCGACTCGCTTGCTGCAAGCCGGCGCGGCGGGTTACCTGACCAAGGGCGCGGG
TCTCAACGAAATGGTGCAGGCCATCCGCCTGGTGTTTGCCGGCCAGCGTTACATCAGCCCGCAGATTGCCCAGCAACTGG
TGTTCAAGTCATTCCAGCCGTCCAGTGATTCACCGTTCGATGCCTTGTCGGAGCGCGAGATCCAGATTGCGCTGATGATT
GTCGGCTGCCAGAAAGTGCAGATCATCTCCGACAAGCTGTGCCTGTCTCCGAAAACCGTGAATACCTACCGTTACCGTAT
CTTCGAGAAGCTCTCGATCAGCAGTGATGTCGAACTGACGTTGCTGGCGGTTCGCCACGGCATGGTCGATGCCAGCGCCT
GA