Detailed information of regulator

Summary

Regulator ID	REG0253
Regulator type	protein
Regulatory pathway (from literatures)	TCS-RR
KEGG pathway (determined by BlastKOALA)	Two-component system (map02020); Biofilm formation - Vibrio cholerae (map05111); Biofilm formation - Pseudomonas aeruginosa (map02025); Biofilm formation - Escherichia coli (map02026)
Related T6SS	T6SS00612 (Type i4a); T6SS00613 (Type i1); T6SS00611 (Type i3)
Strain	Pseudomonas aeruginosa M18
Replicon	chromosome
Sequence	Protein sequence (215 a.a.); Nucleotide sequence (645 bp)
Description	The β-galactosidase activities resulting from gacA mutant and wild-type strain M18 showed T6SS was downregulated in the gacA mutant compared with their expression in M18.
Reference	[1] Wei X, Huang X, Tang L, et al. Global control of GacA in secondary metabolism, primary metabolism, secretion systems, and motility in the rhizobacterium Pseudomonas aeruginosa M18. J Bacteriol. 2013 Aug;195(15):3387-400. doi: 10.1128/JB.00214-13. Epub 2013 May 24. PMID: 23708134

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External database links

Locus tag (Gene)	PAM18_RS12360 (gacA)
Coordinates (Strand)	2625815..2626459 (+)
NCBI ID	489180874
RefSeq	NC_017548
Uniprot ID	V6AEI3_PSEAI, A0A1C7BRH9_PSEAI, A0A1F0J1Y5_9PSED, A0A0H2ZC55_PSEAB, GACA_PSEAE, A6V4J1_PSEA7
KEGG ID	pap:PSPA7_2613, pae:PA2586, pau:PA14_30650
KEGG pathway (determined by BlastKOALA)	map02020; map05111; map02025; map02026
PDB ID	-

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  Target

Target	Regulation (↓/↑)	Target sequence
hsiA1 operon transcript	↓	-
hsiA2 operon transcript	↓	-
hsiA3 operon transcript	↓	-

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Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
Response_reg	PF00072.26	2.8e-28	4..116
GerE	PF00196.21	1.2e-14	148..204

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-214	VIKVLVVDDHDLVRTGITRMLADIEGLQVVGQADCGEDCLKLARELKPDVVLMDVKMPGIGGLEATRKLLRSQPDIKVVVVTVCEEDPFPTRLMQAGAAGYMTKGAGLEEMVQAIRQVFAGQRYISPQIAQQLALKSFQPQQHDSPFDSLSEREIQIALMIANCHKVQSISDKLCLSPKTVNTYRYRIFEKLSITSDVELALLAVRHGMVDAAS

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9941	-	-

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  Protein sequence of regulator: 215 a.a.    .Download


  Nucleotide sequence of regulator: 645 bp    .Download