Detailed information of accessory protein


Summary

Accessory protein ID ACP00020 experimental
Type chaperone
Related T6SS (Type) T6SS00081 (Type ii)
Strain Francisella tularensis subsp. tularensis SCHU S4
Replicon chromosome
Related structure protein FTT_1358c (IglB1); FTT_1357c (IglC1)
Related effector -
Description the T6SS of Francisella requires the FipB protein for proper conformation of IglB and IglC
Reference [1] Qin A, Zhang Y, Clark ME, et al. Components of the type six secretion system are substrates of Francisella tularensis Schu S4 DsbA-like FipB protein. Virulence. 2016 Nov 16;7(8):882-894. Epub 2016 Mar 30. PMID: 27028889
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) FTT_0682c (fipB/dsbA)
Coordinates (Strand) 699171..700016 (-)
NCBI ID 56707802
RefSeq NC_006570
Uniprot ID Q5NGZ4_FRATT
KEGG ID ftu:FTT_0682c
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MQKKSF
Transmembrane helix 7-26 SILVLFVVILLVVLYIFSTT
Outside 27-281 NLNKISPDNKQGLYLVEHAFPQYKVIKTFDTGIHLQAYILEDKKDPTKHSVTFTSEDGAVIVNGELLAWDSNQNKLTSLNNIYANYFTSSPQANNLYLNIKKYATYIQQGSDDAPHKFYAIINPSCSYCNRLFDATQPAINSGELAVRWIPLGALRNSPEIVRSIFNSKDPLEALIKYHKTKTYDQKLTQQNEKAENNLRLSSDIEGFPTIVYKTPQGALKISGGNKLPLTDATIAAKDNINKINEFLLLTSNSF



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9269 - -



  Protein sequence: 282 a.a.    

>ACP00020 NC_006570:c700016-699171 [Francisella tularensis subsp. tularensis SCHU S4]
MQKKSFSILVLFVVILLVVLYIFSTTNLNKISPDNKQGLYLVEHAFPQYKVIKTFDTGIHLQAYILEDKKDPTKHSVTFT
SEDGAVIVNGELLAWDSNQNKLTSLNNIYANYFTSSPQANNLYLNIKKYATYIQQGSDDAPHKFYAIINPSCSYCNRLFD
ATQPAINSGELAVRWIPLGALRNSPEIVRSIFNSKDPLEALIKYHKTKTYDQKLTQQNEKAENNLRLSSDIEGFPTIVYK
TPQGALKISGGNKLPLTDATIAAKDNINKINEFLLLTSNSF*

  Nucleotide sequence: 846 bp    

>ACP00020 NC_006570:c700016-699171 [Francisella tularensis subsp. tularensis SCHU S4]
ATGCAAAAAAAAAGCTTCTCTATTCTAGTTTTATTTGTTGTTATACTATTAGTTGTACTGTATATTTTTAGTACTACTAA
TCTTAATAAAATCTCACCAGATAACAAACAAGGCTTATACCTTGTTGAGCATGCCTTTCCACAATATAAGGTTATAAAGA
CTTTTGACACAGGTATCCATCTACAAGCATATATTCTAGAAGACAAAAAAGATCCAACAAAACATAGTGTTACTTTTACT
AGTGAAGATGGTGCTGTAATTGTTAATGGTGAACTTTTAGCATGGGATAGTAATCAAAACAAGCTAACTAGTTTAAATAA
TATTTATGCAAACTATTTCACTTCATCACCTCAAGCGAACAATCTTTATCTAAACATCAAAAAGTATGCTACATACATCC
AGCAAGGCAGTGATGATGCGCCACATAAGTTCTATGCTATTATAAACCCTAGTTGCAGCTATTGTAATCGTCTATTTGAT
GCGACTCAACCAGCAATTAACTCAGGAGAACTTGCAGTACGCTGGATTCCTTTAGGTGCACTGCGTAATAGCCCAGAGAT
TGTTAGAAGTATTTTTAACTCAAAAGATCCTCTTGAAGCATTGATTAAATATCATAAGACAAAAACATATGATCAGAAAT
TAACTCAACAAAATGAAAAAGCCGAAAATAATTTAAGGTTATCGAGTGATATTGAAGGTTTCCCTACAATTGTTTATAAA
ACACCTCAAGGAGCTTTAAAAATCTCAGGTGGTAACAAGCTTCCACTTACAGATGCTACTATAGCAGCAAAAGATAATAT
CAATAAAATTAATGAATTTTTGCTATTAACCTCAAATAGTTTCTAA