Detailed information of component protein


Summary

Component ID T6CP005178
Component protein type TssC
T6SS ID (Type) T6SS00081 experimental (Type ii)
Strain Francisella tularensis subsp. tularensis SCHU S4
Replicon chromosome
Sequence Protein sequence (507 a.a.); Nucleotide sequence (1521 bp)
Reference
[1] Bröms JE, Meyer L, Sun K, et al. Unique substrates secreted by the type VI secretion system of Francisella tularensis during intramacrophage infection. PLoS One. 2012;7(11):e50473. doi: 10.1371/journal.pone.0050473. Epub 2012 Nov 20. PMID: 23185631
[2] Nano FE, Schmerk C. The Francisella pathogenicity island. Ann N Y Acad Sci. 2007 Jun;1105:122-37. Epub 2007 Mar 29. PMID: 17395722
[3] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
[4] Long ME, Lindemann SR, Rasmussen JA, et al. Disruption of Francisella tularensis Schu S4 iglI, iglJ, and pdpC genes results in attenuation for growth in human macrophages and in vivo virulence in mice and reveals a unique phenotype for pdpC. Infect Immun. 2013 Mar;81(3):850-61. doi: 10.1128/IAI.00822-12. Epub 2012 Dec 28. PMID: 23275090
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) FTT_RS07120
Coordinate (Strand) 1399284..1400804 (-)
NCBI ID WP_003022151.1
RefSeq NC_006570
Uniprot ID UPI000158ADFD
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 1e-95 75..375
VipB_2 PF18945.2 6.6e-38 387..496
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-506 MTINKLSLTDELLNNFGGSTEVDSVLKNIDFDVSDDASKVLSLSTDYNARNLMALSLVLANNDNINNYNQKYIQKVITVIDKLIDLQVNSIISNDEFRALEQEWLKVQEVCQEDYDNVEVSILDVKKEELQYDFERNLYDISSSDFFKKVYVSEFDQYGGEPYGAILGLYNFENTTNDIIWLTGMGMVAKNSHAPFIASIDKSFFGVKDLSEITHIKSFEALLEHPRYKEWNDFRNLDVAAYIGLTVGDFMLRQPYNPENNPVQYKLMEGFNEFVDYDKNESYLWGPASIHLVKNMMRSYDKTRWFQYIRGVESGGYVKNLVACVYDNKGILETKSPLNVLFADYMELSLANIGLIPFVSEKGTSNACFFSVNSAKKVEEFVDGFDSANSRLIANLSYTMCISRISHYIKCVIRDKIGSIVDVESIQKILSDWISEFVTTVYQPTPLEMARYPFRNVSIEVETIPGKPGWYSCKINVIPHIQFEGMNTTMTIDTRLEPELFGTNNN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9908 - -


  Protein sequence: 507 a.a.    

>T6CP005178 NC_006570:c1400804-1399284 [Francisella tularensis subsp. tularensis SCHU S4] [TssC]
MTINKLSLTDELLNNFGGSTEVDSVLKNIDFDVSDDASKVLSLSTDYNARNLMALSLVLANNDNINNYNQKYIQKVITVI
DKLIDLQVNSIISNDEFRALEQEWLKVQEVCQEDYDNVEVSILDVKKEELQYDFERNLYDISSSDFFKKVYVSEFDQYGG
EPYGAILGLYNFENTTNDIIWLTGMGMVAKNSHAPFIASIDKSFFGVKDLSEITHIKSFEALLEHPRYKEWNDFRNLDVA
AYIGLTVGDFMLRQPYNPENNPVQYKLMEGFNEFVDYDKNESYLWGPASIHLVKNMMRSYDKTRWFQYIRGVESGGYVKN
LVACVYDNKGILETKSPLNVLFADYMELSLANIGLIPFVSEKGTSNACFFSVNSAKKVEEFVDGFDSANSRLIANLSYTM
CISRISHYIKCVIRDKIGSIVDVESIQKILSDWISEFVTTVYQPTPLEMARYPFRNVSIEVETIPGKPGWYSCKINVIPH
IQFEGMNTTMTIDTRLEPELFGTNNN*

  Nucleotide sequence: 1521 bp    

>T6CP005178 NC_006570:c1400804-1399284 [Francisella tularensis subsp. tularensis SCHU S4] [TssC]
ATGACAATAAATAAATTAAGTCTCACTGATGAACTTTTAAATAATTTTGGGGGATCTACAGAAGTTGATAGTGTACTCAA
AAATATAGATTTTGATGTTTCAGATGATGCTTCTAAAGTTTTATCTTTATCTACTGACTACAATGCTAGAAACCTTATGG
CGCTATCTTTGGTATTAGCAAATAATGATAATATAAATAATTATAATCAAAAATATATCCAGAAAGTTATTACAGTTATT
GATAAGCTTATTGATTTACAAGTTAATTCTATTATATCTAATGATGAGTTTAGAGCACTTGAGCAAGAATGGCTAAAGGT
GCAAGAGGTTTGTCAAGAAGACTATGATAATGTTGAAGTAAGTATATTAGATGTAAAAAAAGAAGAGCTACAATATGATT
TCGAGAGAAATTTATATGATATATCTAGTAGTGACTTTTTCAAAAAAGTTTACGTTTCAGAATTTGATCAATATGGTGGC
GAACCTTATGGCGCAATATTAGGATTGTATAATTTTGAAAATACCACAAATGATATAATTTGGTTGACTGGAATGGGTAT
GGTGGCAAAGAATTCTCATGCACCATTTATTGCATCAATTGATAAGTCATTCTTTGGTGTTAAGGATTTATCAGAAATCA
CTCATATAAAAAGTTTTGAAGCTTTGCTTGAGCATCCTAGATATAAAGAGTGGAATGATTTTAGAAACCTTGATGTTGCT
GCATATATAGGTTTGACCGTAGGTGATTTTATGTTGCGGCAACCATATAATCCTGAGAATAATCCAGTTCAGTATAAACT
TATGGAAGGCTTTAATGAGTTTGTTGATTATGATAAGAATGAAAGTTATCTATGGGGACCTGCTTCAATTCATCTAGTTA
AGAATATGATGAGATCTTATGATAAAACTAGATGGTTCCAATATATAAGAGGAGTTGAGAGTGGTGGTTATGTAAAGAAC
TTGGTAGCTTGCGTATATGATAATAAAGGCATTCTAGAAACTAAGTCACCTTTAAATGTATTATTCGCTGATTATATGGA
GTTATCACTTGCAAATATTGGTTTAATACCATTTGTAAGTGAAAAAGGTACTAGTAACGCTTGTTTCTTTAGTGTAAATT
CTGCTAAAAAAGTCGAAGAATTTGTAGATGGATTTGACTCAGCAAACTCAAGATTAATTGCTAACCTTTCTTACACTATG
TGTATATCGAGAATATCTCATTATATTAAATGTGTAATTAGAGATAAGATTGGTAGTATTGTGGATGTCGAGTCGATTCA
AAAAATTCTTTCTGATTGGATATCAGAATTTGTCACCACAGTCTATCAACCAACCCCTTTAGAAATGGCGAGATATCCTT
TCAGAAACGTTTCTATCGAGGTTGAAACCATACCGGGTAAGCCTGGCTGGTATTCATGCAAAATAAATGTAATTCCCCAC
ATTCAATTTGAAGGAATGAATACTACAATGACTATAGATACTAGGCTTGAACCAGAATTATTCGGTACAAATAATAACTA
A