Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFT96_RS07020 Genome accession   NZ_CP155942
Coordinates   1658128..1659165 (+) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain DAPP-PG 308     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1653128..1664165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFT96_RS06990 (ABFT96_06985) - 1653562..1654101 (-) 540 WP_011037889.1 hypothetical protein -
  ABFT96_RS06995 (ABFT96_06990) - 1654155..1654463 (-) 309 WP_016944107.1 DUF3861 domain-containing protein -
  ABFT96_RS07000 (ABFT96_06995) - 1654556..1655146 (-) 591 WP_011037887.1 M23 family metallopeptidase -
  ABFT96_RS07005 (ABFT96_07000) - 1655290..1655712 (-) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFT96_RS07010 (ABFT96_07005) proC 1656257..1657117 (-) 861 WP_016944106.1 pyrroline-5-carboxylate reductase -
  ABFT96_RS07015 (ABFT96_07010) - 1657158..1657841 (-) 684 WP_012437802.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFT96_RS07020 (ABFT96_07015) pilT 1658128..1659165 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFT96_RS07025 (ABFT96_07020) pilU 1659298..1660428 (+) 1131 WP_012437803.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFT96_RS07030 (ABFT96_07025) - 1660708..1661382 (+) 675 WP_014508435.1 YitT family protein -
  ABFT96_RS07035 (ABFT96_07030) - 1661396..1662295 (-) 900 WP_012437805.1 DUF72 domain-containing protein -
  ABFT96_RS07040 (ABFT96_07035) - 1662518..1663795 (-) 1278 WP_012437806.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=999988 ABFT96_RS07020 WP_003489919.1 1658128..1659165(+) (pilT) [Xanthomonas campestris pv. campestris strain DAPP-PG 308]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=999988 ABFT96_RS07020 WP_003489919.1 1658128..1659165(+) (pilT) [Xanthomonas campestris pv. campestris strain DAPP-PG 308]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCCTTGGACCATAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGT
TTTCGCGTCAATGCGTTCAATCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACCCTGGA
AGACCTGGGCTGCCCGCCGATCTTTCGCCAGTTGATCGACCAGCCGCAGGGTCTGATCCTGGTGACCGGCCCGACCGGGT
CGGGCAAGTCGACCACGCTTGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCCCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCATCCGCCTGG
CACTGACCGCCGCGGAAACCGGCCATCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCGGTGATTTCGCAGGC
ATTGCTCAAGAAAGTGGGCGGCGGGCGTACCGCAGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment