Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU47_RS16650 Genome accession   NZ_CP155926
Coordinates   3773952..3775661 (-) Length   569 a.a.
NCBI ID   WP_323475836.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain bglFP 6772     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3768952..3780661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU47_RS16630 (ABFU47_16565) - 3769266..3770876 (+) 1611 WP_014508702.1 PAS domain-containing sensor histidine kinase -
  ABFU47_RS16635 (ABFU47_16570) pilR 3771085..3772479 (+) 1395 WP_043921909.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU47_RS16640 (ABFU47_16575) - 3772677..3773033 (+) 357 WP_211914179.1 hypothetical protein -
  ABFU47_RS16645 (ABFU47_16580) - 3772978..3773769 (+) 792 WP_202796265.1 toxin -
  ABFU47_RS16650 (ABFU47_16585) pilB 3773952..3775661 (-) 1710 WP_323475836.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU47_RS16655 (ABFU47_16590) comP 3775834..3776247 (-) 414 WP_323475832.1 pilin Machinery gene
  ABFU47_RS16660 (ABFU47_16595) comP 3776380..3776802 (-) 423 WP_323475831.1 pilin Machinery gene
  ABFU47_RS16665 (ABFU47_16600) pilC 3777129..3778391 (+) 1263 WP_323475830.1 type II secretion system F family protein Machinery gene
  ABFU47_RS16670 (ABFU47_16605) - 3778398..3779261 (+) 864 WP_323475829.1 A24 family peptidase -
  ABFU47_RS16675 (ABFU47_16610) coaE 3779275..3779898 (+) 624 WP_116670565.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61883.93 Da        Isoelectric Point: 5.6021

>NTDB_id=999744 ABFU47_RS16650 WP_323475836.1 3773952..3775661(-) (pilB) [Xanthomonas campestris pv. raphani strain bglFP 6772]
MGITGIARRLVQDGVLDEVNARAAMDQAASAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDASQNAVKLVSE
ELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASSAALGSSLGDDDDEMGDL
DVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLSQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKLFLDAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKATLPEHALLAEGF
TQQQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALVKAAHGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=999744 ABFU47_RS16650 WP_323475836.1 3773952..3775661(-) (pilB) [Xanthomonas campestris pv. raphani strain bglFP 6772]
GTGGGAATTACGGGGATAGCGCGACGGTTAGTTCAAGACGGCGTGCTTGACGAAGTTAATGCTCGGGCAGCAATGGATCA
GGCTGCCTCGGCGAAAGTTCCATTACCACAGTGGTTTGCTGAGAAGAAGCTAGTTTCAGCCGCTCAGCTAGCTGCAGCCA
ATGCAGTCGAGTTCGGCATGCCGCTGATGGACGTGTCGGTCTTCGACGCCAGCCAAAACGCAGTCAAGCTGGTCAGTGAG
GAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGTAAGCAACCCGACCCA
GACCAGGGCACTGGACGATATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGGTTGATGAGGACCAGATCCGTC
GCACCTTGGAGCAGTGGCAGGCGAGCAGTGCGGCGCTCGGCTCTTCGCTCGGTGACGACGACGACGAAATGGGGGATCTG
GACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCCATTTCGAGCCCTATGAAGACGACT
ATCGGGTGCGGTTGCGCATCGATGGCCTGCTGAAGAACGTGGCCAAGGCGCCGGTGAAGCTGAGTCAGCGCATCGCGGCG
CGCTTGAAGGTTATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGTATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTGAGCACCCTGCCGACCTTGTTCGGCGAGAAAGTGGTGCTGCGTATTCTGGACGGCA
GCGCGGCCAAGCTGGGTATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTGTTCCTGGATGCGATCCATAAGCCG
TACGGCATGGTGCTGGTGACCGGTCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATACGGCGCTGGGCATCTTGAACGA
CGAGACGCGCAATATCTCTACGGCCGAGGATCCGGTGGAAATCCGCCTGCCTGGCGTCAACCAGGTGCAGCAGAACAACA
AGCGCGGCATGACCTTCGCTGCGGCCTTGCGCTCGTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATTCGC
GACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACAGGCCACATGGTGCTGTCGACGCTGCATACCAACGATGC
GCCGCAGACCATCGCGCGCTTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCTTGGTCATCGCGC
AGCGCCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGGCCACCTTGCCCGAGCATGCGTTGCTGGCCGAAGGGTTC
ACGCAACAGCAGATCGCTGCAGGTATCGAGCTGTATGAGGCGGTGGGCTGCGACGAGTGCACGGAAGGCTACAAGGGCCG
TACCGGTATCTACCAGGTCATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAAGGCGGCAATGCGATGCAGATCG
CCGAGGCGGCGCAGAAGATCGGCATCCGCGATCTACGCCAGTCGGCGCTGGTCAAGGCGGCGCATGGTGTGACGAGCCTG
GCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

99.824

0.561
  pilB Acinetobacter baylyi ADP1

55.282

99.824

0.552
  pilB Legionella pneumophila strain ERS1305867

51.845

100

0.518
  pilB Vibrio cholerae strain A1552

49.12

99.824

0.49
  pilF Neisseria gonorrhoeae MS11

48.944

99.824

0.489
  pilB Vibrio campbellii strain DS40M4

45.518

100

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

45.263

100

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

92.97

0.367
  pilF Thermus thermophilus HB27

39.808

91.388

0.364

Multiple sequence alignment