Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAHK14_RS09260 Genome accession   NZ_CP155576
Coordinates   1855513..1856712 (+) Length   399 a.a.
NCBI ID   WP_065256545.1    Uniprot ID   A0A1B8Q465
Organism   Moraxella sp. K1664     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1850513..1861712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHK14_RS09225 (AAHK14_09225) - 1850670..1851035 (+) 366 WP_065256551.1 gamma-glutamylcyclotransferase family protein -
  AAHK14_RS09230 (AAHK14_09230) - 1851066..1851539 (+) 474 WP_065256550.1 DUF4303 domain-containing protein -
  AAHK14_RS09235 (AAHK14_09235) - 1851579..1851773 (+) 195 WP_065256549.1 hypothetical protein -
  AAHK14_RS09240 (AAHK14_09240) - 1851852..1852022 (+) 171 WP_065256548.1 acetyltransferase -
  AAHK14_RS09245 (AAHK14_09245) - 1852022..1852408 (+) 387 WP_065256547.1 type II toxin-antitoxin system death-on-curing family toxin -
  AAHK14_RS09250 (AAHK14_09250) nuoC 1852674..1854449 (+) 1776 WP_227514751.1 NADH-quinone oxidoreductase subunit C/D -
  AAHK14_RS09255 (AAHK14_09255) - 1854456..1855460 (+) 1005 WP_065256546.1 hypothetical protein -
  AAHK14_RS09260 (AAHK14_09260) pilU 1855513..1856712 (+) 1200 WP_065256545.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAHK14_RS09265 (AAHK14_09265) - 1856896..1857174 (+) 279 WP_065256544.1 hypothetical protein -
  AAHK14_RS09270 (AAHK14_09270) - 1857237..1857728 (+) 492 WP_156065181.1 SMI1/KNR4 family protein -
  AAHK14_RS09275 (AAHK14_09275) nuoE 1857845..1858354 (+) 510 WP_062499124.1 NADH-quinone oxidoreductase subunit NuoE -
  AAHK14_RS09280 (AAHK14_09280) nuoF 1858351..1859769 (+) 1419 WP_194092634.1 NADH-quinone oxidoreductase subunit NuoF -

Sequence

Protein


Download         Length: 399 a.a.        Molecular weight: 44963.31 Da        Isoelectric Point: 5.7231

>NTDB_id=997924 AAHK14_RS09260 WP_065256545.1 1855513..1856712(+) (pilU) [Moraxella sp. K1664]
MTTHHISEESLIAFAEETLNEAKGIMYNMLAKVVEIGASDLFITADFPPSVKHQGLMKPLGKQVLSADKTKLFAYSIMND
YQRDEFEREMECNFAINVPNVSRFRVNVFIQQQQVGMVIRTIAAEIPNFEKLRLPQKLKDVILEKRGLVLVVGGTGSGKS
TSLAAMIDYRNENSAGHIITVEDPVEYVHKHKKSMITHREVGVDTHSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAF
AETGHLCLGTLHANNANQTLDRIINFFPEERRQQLLMDLSSNMKAIISQRLIRTEDGQGRRAAVEIMLNTPLVADLILKS
EMHELKAIMTKSRELGMQTFDQALFDLYDEGAISYDEAIRNADSPNELRLQIKLKGTRRDGEQEVASALSLHADESDDD

Nucleotide


Download         Length: 1200 bp        

>NTDB_id=997924 AAHK14_RS09260 WP_065256545.1 1855513..1856712(+) (pilU) [Moraxella sp. K1664]
ATGACCACACACCACATCTCCGAAGAATCACTCATCGCCTTTGCCGAAGAGACCCTAAACGAAGCCAAAGGTATCATGTA
TAATATGCTTGCCAAAGTCGTTGAGATTGGGGCATCTGACCTGTTTATCACGGCAGATTTTCCGCCGAGCGTCAAACATC
AAGGCTTGATGAAACCGCTTGGCAAACAGGTGCTAAGTGCCGATAAGACCAAATTGTTTGCATACAGTATCATGAATGAT
TATCAGCGTGATGAATTTGAACGTGAAATGGAATGTAACTTTGCCATTAACGTGCCAAATGTCAGCCGTTTTCGTGTCAA
TGTCTTTATTCAGCAACAGCAAGTGGGCATGGTCATTCGTACCATTGCCGCCGAGATTCCCAATTTTGAAAAGCTAAGAT
TACCCCAAAAGCTCAAAGACGTGATTTTGGAAAAGCGGGGTTTGGTGCTGGTGGTGGGCGGTACAGGTTCGGGTAAATCC
ACGAGCTTGGCGGCGATGATTGACTACCGTAACGAAAACAGTGCAGGGCATATCATCACAGTAGAAGACCCTGTGGAATA
TGTGCATAAGCACAAAAAATCGATGATTACGCACCGTGAAGTGGGCGTGGATACGCACTCATGGCACCATGCGTTGAAAA
ATACGCTCCGCCAAGCCCCTGATGTGATTTTGATTGGTGAGATTCGAGATACCGAGACCATGGAACACGCCATTGCCTTT
GCTGAGACGGGGCATTTGTGTCTAGGGACGCTCCACGCCAACAACGCCAACCAAACGCTTGACCGTATCATCAACTTTTT
CCCCGAAGAGCGTCGCCAACAGCTCCTTATGGATTTGTCCAGCAACATGAAAGCGATTATCTCACAGCGACTCATTCGCA
CCGAAGACGGGCAGGGACGGCGTGCGGCGGTGGAGATTATGCTGAACACGCCATTGGTGGCGGATTTGATTTTAAAAAGT
GAAATGCATGAGTTAAAGGCGATTATGACCAAAAGCCGTGAGTTGGGTATGCAGACCTTTGACCAAGCGTTGTTTGACCT
ATATGATGAAGGGGCTATCTCGTACGATGAAGCGATTCGCAATGCCGACTCGCCCAACGAGCTACGCCTACAAATCAAGC
TCAAAGGCACCAGACGAGACGGCGAGCAGGAAGTGGCGAGTGCGTTATCGCTCCATGCTGATGAGAGTGATGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(103-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B8Q465

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.491

92.982

0.544
  pilU Acinetobacter baylyi ADP1

57.714

87.719

0.506
  pilU Vibrio cholerae strain A1552

53.846

84.712

0.456
  pilT Acinetobacter nosocomialis M2

44.51

84.461

0.376
  pilT Acinetobacter baumannii D1279779

44.51

84.461

0.376
  pilT Acinetobacter baumannii strain A118

44.51

84.461

0.376
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.109

85.464

0.368
  pilT Acinetobacter baylyi ADP1

43.323

84.461

0.366
  pilT Pseudomonas aeruginosa PAK

42.73

84.461

0.361

Multiple sequence alignment