Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMG15_RS11070 Genome accession   NZ_AP027055
Coordinates   2204451..2205671 (+) Length   406 a.a.
NCBI ID   WP_281788646.1    Uniprot ID   -
Organism   Limnohabitans sp. INBF002     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2199451..2210671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG15_RS11030 (LINBF2_21900) cgtA 2199874..2200944 (-) 1071 WP_108358288.1 Obg family GTPase CgtA -
  QMG15_RS11035 (LINBF2_21910) rpmA 2201009..2201266 (-) 258 WP_104796974.1 50S ribosomal protein L27 -
  QMG15_RS11040 (LINBF2_21920) rplU 2201279..2201590 (-) 312 WP_104796975.1 50S ribosomal protein L21 -
  QMG15_RS11045 (LINBF2_21930) - 2201783..2202712 (+) 930 WP_281790129.1 polyprenyl synthetase family protein -
  QMG15_RS11050 (LINBF2_21940) - 2202727..2203128 (+) 402 WP_281788643.1 hypothetical protein -
  QMG15_RS11055 (LINBF2_21950) - 2203147..2203473 (-) 327 WP_281788644.1 hypothetical protein -
  QMG15_RS11060 (LINBF2_21960) - 2203497..2203877 (-) 381 WP_281788645.1 H-NS histone family protein -
  QMG15_RS11065 (LINBF2_21970) - 2203990..2204262 (-) 273 WP_108358292.1 HU family DNA-binding protein -
  QMG15_RS11070 (LINBF2_21980) pilB 2204451..2205671 (+) 1221 WP_281788646.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  QMG15_RS11075 (LINBF2_21990) - 2205871..2206878 (+) 1008 WP_281788647.1 type II secretion system F family protein -
  QMG15_RS11080 (LINBF2_22000) - 2206875..2207684 (+) 810 WP_281788648.1 A24 family peptidase -
  QMG15_RS11085 (LINBF2_22010) coaE 2207695..2208318 (+) 624 WP_281788649.1 dephospho-CoA kinase -
  QMG15_RS11090 (LINBF2_22020) zapD 2208392..2209144 (+) 753 WP_108358297.1 cell division protein ZapD -
  QMG15_RS11095 (LINBF2_22030) - 2209148..2209348 (+) 201 WP_108358298.1 DNA gyrase inhibitor YacG -
  QMG15_RS11100 (LINBF2_22040) - 2209362..2209832 (-) 471 WP_281788650.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44507.13 Da        Isoelectric Point: 7.0855

>NTDB_id=99415 QMG15_RS11070 WP_281788646.1 2204451..2205671(+) (pilB) [Limnohabitans sp. INBF002]
MPPPHIAPSAIPEQSAVQTLDLLLHRAFEQLASDIHVESGEDFFRVRFRIDGQLQIAATPTLSLRDPVVSRLKVLARMDI
AEKRVPQDGRIQYPYKDQTIDLRVSSLPTLHGEKIVVRILNFSRERPGLAALGYEPDDRTKLLQALGRPHGLILMTGPTG
SGKTLSLYSCLELLNRAEVNISTVEDPSEIHLPGANQVNINERAGLTFATTLRALLRQDPDVIMVGEIRDLETAEIAIQA
AQTGHLVLSTLHTNDAPSTLARLRHMGIAAFNVAASVNLITAQRLIRRLCDKCKSPLDAEQTAFFFNTLTPQDSQAIPAD
SRAHAALYKAVGCHACDKGYKGRIGIYQVMPVSDAMQTCIIRDNDTQTLAAQAALEGVRTLRQAGWLKVLQGITTMDEVM
ALTNHG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=99415 QMG15_RS11070 WP_281788646.1 2204451..2205671(+) (pilB) [Limnohabitans sp. INBF002]
ATGCCACCCCCACACATCGCGCCATCCGCAATACCGGAACAATCTGCCGTCCAAACGCTGGATCTGCTCCTGCATCGCGC
ATTTGAACAACTTGCGTCAGACATTCATGTCGAAAGTGGCGAGGATTTCTTTCGCGTGCGGTTTCGCATTGACGGGCAAT
TACAAATCGCAGCCACCCCTACCCTCAGTTTGCGAGATCCGGTTGTTTCGCGCCTCAAGGTTTTGGCTCGGATGGATATT
GCGGAGAAGCGTGTACCGCAAGATGGCCGAATTCAGTATCCCTACAAAGACCAAACCATTGATTTACGGGTCAGCTCTTT
GCCCACGTTGCATGGCGAGAAAATCGTGGTTCGAATTTTGAATTTCTCCCGTGAAAGGCCGGGGCTGGCTGCGTTGGGGT
ATGAGCCAGATGACCGCACCAAATTACTTCAAGCCCTAGGCCGCCCACATGGCCTGATTTTGATGACGGGGCCAACGGGC
TCTGGCAAAACCTTGTCTCTTTACAGCTGCCTTGAATTATTAAACCGGGCAGAAGTCAATATCTCAACGGTTGAGGATCC
GTCAGAAATTCACCTCCCTGGCGCGAACCAAGTCAATATCAATGAGCGCGCTGGATTGACTTTTGCCACCACGCTTCGGG
CCTTGCTGCGACAAGATCCTGACGTGATCATGGTGGGTGAAATTCGCGATTTAGAAACTGCCGAAATTGCCATTCAAGCG
GCACAAACTGGCCATTTAGTTCTCTCAACACTGCATACCAACGACGCCCCCAGCACCTTAGCGCGGCTGCGCCACATGGG
CATTGCGGCATTCAACGTGGCTGCGAGCGTCAACCTCATCACAGCACAACGGCTGATCCGACGTTTGTGTGACAAATGCA
AAAGCCCTTTAGATGCAGAACAAACCGCCTTCTTTTTCAACACATTAACGCCGCAAGACAGCCAAGCCATTCCAGCCGAC
TCACGCGCACACGCCGCACTGTACAAAGCGGTCGGCTGCCACGCCTGCGACAAAGGCTACAAAGGACGCATCGGCATTTA
CCAAGTCATGCCTGTCAGTGATGCCATGCAAACATGCATCATCCGTGACAACGACACCCAAACCTTGGCTGCGCAAGCCG
CACTGGAGGGCGTGCGTACCTTAAGGCAAGCCGGATGGTTGAAGGTCTTGCAAGGCATCACCACCATGGACGAGGTCATG
GCCCTCACCAACCATGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(24-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.792

97.044

0.512
  pilB Acinetobacter baylyi ADP1

52.821

96.059

0.507
  pilF Neisseria gonorrhoeae MS11

52.727

94.828

0.5
  pilB Legionella pneumophila strain ERS1305867

49.749

98.03

0.488
  pilB Vibrio campbellii strain DS40M4

48.429

94.089

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

48.294

93.842

0.453
  pilB Vibrio cholerae strain A1552

48.936

92.611

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.325

97.783

0.424
  pilB/pilB1 Synechocystis sp. PCC 6803

42.469

99.754

0.424
  pilF Thermus thermophilus HB27

41.96

98.03

0.411
  pilB Haemophilus influenzae Rd KW20

41.902

95.813

0.401
  pilB Haemophilus influenzae 86-028NP

41.645

95.813

0.399
  pilB Glaesserella parasuis strain SC1401

40.885

94.581

0.387
  ctsE Campylobacter jejuni subsp. jejuni 81-176

37.852

96.305

0.365

Multiple sequence alignment