Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   AAIS09_RS09575 Genome accession   NZ_CP154595
Coordinates   1872717..1873658 (-) Length   313 a.a.
NCBI ID   WP_000249550.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain serotype 4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1867717..1878658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIS09_RS09535 (AAIS09_09520) comYH 1868210..1869163 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  AAIS09_RS09540 (AAIS09_09525) - 1869224..1869811 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  AAIS09_RS09545 (AAIS09_09530) comGG/cglG 1869948..1870361 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  AAIS09_RS09550 (AAIS09_09535) comGF/cglF 1870339..1870800 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  AAIS09_RS09555 (AAIS09_09540) comGE/cglE 1870763..1871065 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  AAIS09_RS09560 (AAIS09_09545) comGD/cglD 1871028..1871432 (-) 405 WP_000588006.1 competence type IV pilus minor pilin ComGD Machinery gene
  AAIS09_RS09565 (AAIS09_09550) comGC/cglC 1871425..1871751 (-) 327 WP_000738638.1 comG operon protein ComGC Machinery gene
  AAIS09_RS09570 (AAIS09_09555) comGB/cglB 1871753..1872769 (-) 1017 WP_075270915.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAIS09_RS09575 (AAIS09_09560) comGA/cglA/cilD 1872717..1873658 (-) 942 WP_000249550.1 competence type IV pilus ATPase ComGA Machinery gene
  AAIS09_RS09580 (AAIS09_09565) - 1873734..1874099 (-) 366 WP_000286415.1 DUF1033 family protein -
  AAIS09_RS09585 (AAIS09_09570) - 1874250..1875308 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  AAIS09_RS09590 (AAIS09_09575) nagA 1875471..1876622 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  AAIS09_RS09595 (AAIS09_09580) - 1876775..1878592 (-) 1818 WP_001220847.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35516.43 Da        Isoelectric Point: 6.0083

>NTDB_id=993719 AAIS09_RS09575 WP_000249550.1 1872717..1873658(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain serotype 4]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=993719 AAIS09_RS09575 WP_000249550.1 1872717..1873658(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain serotype 4]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

78.387

99.042

0.776
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment