Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AAIA70_RS13485 Genome accession   NZ_CP154264
Coordinates   2938042..2939265 (+) Length   407 a.a.
NCBI ID   WP_005446256.1    Uniprot ID   K5TX94
Organism   Vibrio harveyi strain 45T2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2933042..2944265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIA70_RS13465 (AAIA70_13465) ampD 2934063..2934614 (-) 552 WP_045488718.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AAIA70_RS13470 (AAIA70_13470) nadC 2934707..2935594 (+) 888 WP_343290998.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAIA70_RS13475 (AAIA70_13475) pilA 2935859..2936317 (+) 459 WP_017189133.1 pilin Machinery gene
  AAIA70_RS13480 (AAIA70_13480) pilB 2936323..2938008 (+) 1686 WP_343290999.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAIA70_RS13485 (AAIA70_13485) pilC 2938042..2939265 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  AAIA70_RS13490 (AAIA70_13490) pilD 2939338..2940207 (+) 870 WP_045488712.1 A24 family peptidase Machinery gene
  AAIA70_RS13495 (AAIA70_13495) coaE 2940208..2940822 (+) 615 WP_343291000.1 dephospho-CoA kinase -
  AAIA70_RS13500 (AAIA70_13500) zapD 2940850..2941590 (+) 741 WP_343291001.1 cell division protein ZapD -
  AAIA70_RS13505 (AAIA70_13505) yacG 2941647..2941847 (+) 201 WP_343291002.1 DNA gyrase inhibitor YacG -
  AAIA70_RS13510 (AAIA70_13510) rplS 2942214..2942567 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  AAIA70_RS13515 (AAIA70_13515) trmD 2942609..2943352 (-) 744 WP_005446247.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  AAIA70_RS13520 (AAIA70_13520) rimM 2943380..2943928 (-) 549 WP_005446245.1 ribosome maturation factor RimM -
  AAIA70_RS13525 (AAIA70_13525) rpsP 2943957..2944205 (-) 249 WP_004410028.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44945.20 Da        Isoelectric Point: 10.3829

>NTDB_id=992791 AAIA70_RS13485 WP_005446256.1 2938042..2939265(+) (pilC) [Vibrio harveyi strain 45T2]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEVEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITVFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTKVIPEFEKMFTGFGAELPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLTARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=992791 AAIA70_RS13485 WP_005446256.1 2938042..2939265(+) (pilC) [Vibrio harveyi strain 45T2]
ATGAAAACCATCGCTCCACAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGAAAAAAAACCTCTGGTAACGT
GCTCGCAATGACAGAAGTCGAGGTAAGAGAGCGCCTTGATGCTCAGCACATCAAGATAAAAAAACTCAAAAAAGGCAGTA
TTTCTTTTATCACCAAGATAAGTCATCGAGTAAAAGGCAAAGACATTACGGTGTTTACTCGTCAAATCGCCACCATGTTG
ATGACTGGCGTCCCTATTGTTCAGGCACTCAAGTTAGTTTCAGAGAACCATAAAAAAGCTGAAATGAAATCGATCCTAAT
GAGTGTGACGCGAGCGGTAGAAGCAGGGACACCGATGTCGAAAGCCATGCGTACCGCTAGCACGCACTTTGATCCACTTT
ACACCGACTTAATCGCAACAGGTGAGCAGTCCGGTAACTTATCCCAGGTCTTTGAGCGTCTGGCGACCTACCGAGAAAAA
AGCGAGCAGTTGCGTGCAAAGGTTATTAAAGCGCTCATTTACCCGGCGATGGTGGTATTAGTAGCACTTGGGGTTTCTTA
TTTAATGCTGACAAAGGTTATTCCTGAGTTTGAAAAAATGTTTACAGGTTTTGGTGCCGAATTACCCGCCTTCACCCAGA
TGGTACTCAACCTTTCTGCTTGGACCCAAAATTGGGGTCCCTTCATTGGGCTGAGCTTTGTGAGCTTACTCATTTCTGGC
AAAATCATGTCACAACGCTCCGACTCATTTCGTCTTTTGACTGCACGGTCGAGTTTAAGAGTACCTATTTTAGGACCAGT
TTTATCCAAAGCTGCCATTGCGAAGTTTAGCCGTACTTTGGCGACCAGTTTTAGCGCAGGTATTCCTATCCTAACCGCCT
TGAAAACTACCTCAAAAACCTCTGGTAACATGCACTATCAATTGGCAATCGAAGAGGTATATCGAGATACTGCCGCTGGT
ATGCCAATGTACATTGCCATGCGTAACTGTAACGTCTTCCCAGAATTGGTATTGCAAATGGTCATGATTGGTGAAGAGTC
AGGTCGCTTAGATGACATGCTCAACAAGATAGCCGCTATTTATGAGTTCGAAGTCGATAACACCGTCGACAACCTAAGTA
AGATTTTGGAACCGTTGATCATTGTTTTTCTGGGTGTCGTTGTCGGTGGTTTGGTGACAGCAATGTACTTACCAATCTTT
AACTTGATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K5TX94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

98.28

100

0.983
  pilC Vibrio cholerae strain A1552

74

98.28

0.727
  pilC Acinetobacter baylyi ADP1

42.462

97.789

0.415
  pilC Pseudomonas stutzeri DSM 10701

41.414

97.297

0.403
  pilC Acinetobacter baumannii D1279779

40.447

99.017

0.4
  pilG Neisseria gonorrhoeae MS11

39.558

100

0.396
  pilC Legionella pneumophila strain ERS1305867

39.163

99.754

0.391
  pilG Neisseria meningitidis 44/76-A

38.725

100

0.388

Multiple sequence alignment