Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAIA70_RS13480 Genome accession   NZ_CP154264
Coordinates   2936323..2938008 (+) Length   561 a.a.
NCBI ID   WP_343290999.1    Uniprot ID   -
Organism   Vibrio harveyi strain 45T2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2931323..2943008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIA70_RS13460 (AAIA70_13460) pdhR 2932891..2933658 (-) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AAIA70_RS13465 (AAIA70_13465) ampD 2934063..2934614 (-) 552 WP_045488718.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AAIA70_RS13470 (AAIA70_13470) nadC 2934707..2935594 (+) 888 WP_343290998.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAIA70_RS13475 (AAIA70_13475) pilA 2935859..2936317 (+) 459 WP_017189133.1 pilin Machinery gene
  AAIA70_RS13480 (AAIA70_13480) pilB 2936323..2938008 (+) 1686 WP_343290999.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAIA70_RS13485 (AAIA70_13485) pilC 2938042..2939265 (+) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  AAIA70_RS13490 (AAIA70_13490) pilD 2939338..2940207 (+) 870 WP_045488712.1 A24 family peptidase Machinery gene
  AAIA70_RS13495 (AAIA70_13495) coaE 2940208..2940822 (+) 615 WP_343291000.1 dephospho-CoA kinase -
  AAIA70_RS13500 (AAIA70_13500) zapD 2940850..2941590 (+) 741 WP_343291001.1 cell division protein ZapD -
  AAIA70_RS13505 (AAIA70_13505) yacG 2941647..2941847 (+) 201 WP_343291002.1 DNA gyrase inhibitor YacG -
  AAIA70_RS13510 (AAIA70_13510) rplS 2942214..2942567 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61987.81 Da        Isoelectric Point: 5.2849

>NTDB_id=992790 AAIA70_RS13480 WP_343290999.1 2936323..2938008(+) (pilB) [Vibrio harveyi strain 45T2]
MLTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLSSGFFQSHELTEHLSSIFGLSCTSLSQYEYSSLCQKLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFKELTAAIRRLYGRALGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYESMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDHQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKTEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPHCRLPQEPNSQLQHIGITSTED
IFQANPDGCNECTQGYSGRTGIYEVMKFDESLSNALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=992790 AAIA70_RS13480 WP_343290999.1 2936323..2938008(+) (pilB) [Vibrio harveyi strain 45T2]
ATGCTAACCAACCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGACTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTGCTCAGTTCTGGCTTTTTTCAATCTCACGAATTGACTGAGCACCTGA
GTTCTATCTTTGGACTTTCCTGCACATCACTCAGTCAGTATGAGTACTCGTCGCTGTGCCAAAAGCTTGGCTTACGCGAT
CTGATCACTCGACATAATGCCTTGCCACTCAATCGCACATCTTCGACATTAATACTCGCGGTTGCAGACCCAACTAACTT
ACAAGCGGAAGATGACTTTCGTTTTGCTACCGGTTTACAAGTCGAGCTAGTTCTGGCGGACTTTAAAGAACTCACGGCAG
CCATTCGTCGTTTATATGGTCGTGCACTCGGTCAAGAAAAGTCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCAGGT
CTCGTTGATATTGGTGAGGACGAAATTGAGAACATTGAAGATCTCAGCCAAGATGAATCGCCTGTCAGTCGTTATATTAA
CCAAATTCTGCTCGATGCCGTTCGTAAAGGGGCTTCCGATATTCACTTCGAGCCCTATGAAAGCATGTATCGAGTGCGTT
TGCGTTGCGATGGCATCTTGATCGAAATTCAACAGCCTCCGAGCCATTTAAGCCGCCGCCTTTCCGCACGTATAAAAATC
CTATCTAAGCTTGATATCGCTGAGCGCCGTTTACCACAGGATGGTCGTATCAAGCTCAAATTAAACCAAGACACCGCGAT
TGACATGCGAGTCTCCACGCTGCCAACTCTGTTTGGGGAGAAAATCGTACTACGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAACTCGGATACAGCGATCATCAAAAGCAATTGTATTTAGAAGCACTGCGCCGCCCACAAGGCATGATC
CTAATGACAGGACCAACGGGCAGCGGCAAAACGGTTTCTCTTTACACTGGTTTGAGCATTCTCAATAAAACCGAGATTAA
CATTTCCACCGCTGAAGACCCCGTTGAGATCAACTTACCCGGCATTAACCAAGTTCAAGTTCAACCTAAGATCGGCTTTG
GCTTTGCAGAAGCGCTTCGCTCTTTCTTGCGTCAAGATCCCGATGTAGTCATGGTGGGTGAGATCCGAGATCTCGACACG
GCAGAAATCGCGATCAAAGCGTCACAAACTGGGCACTTAGTCCTTTCTACCCTGCATACCAACTCCGCCGCCGAGACGGT
TATTCGCTTATCAAATATGGGGGTAGAAAGCTTTAACCTCGCATCCTCTCTTAGCTTGATCATCGCTCAGCGTTTAGCTC
GAAAACTTTGTCCACATTGCAGACTACCTCAAGAACCAAACAGCCAACTTCAACATATTGGAATTACCAGCACTGAAGAT
ATCTTCCAAGCGAACCCTGACGGTTGTAACGAATGCACCCAAGGATACTCTGGTCGCACAGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTGTCAAATGCGCTCATCAAAGGTGCTTCAGTGCATGAATTAGAAAAGCTAGCGATTGCCAATGGTA
TGCAGACTCTACAAATGTCCGGCATTGAAAAGCTCAAACAAGGCGTCACCAGCTTTAGTGAGCTGCAACGCGTTCTCTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

96.257

100

0.963
  pilB Vibrio parahaemolyticus RIMD 2210633

89.483

100

0.895
  pilB Vibrio cholerae strain A1552

72.954

100

0.731
  pilB Acinetobacter baylyi ADP1

48.294

99.287

0.479
  pilB Acinetobacter baumannii D1279779

51.838

92.157

0.478
  pilB Legionella pneumophila strain ERS1305867

48.155

96.613

0.465
  pilF Neisseria gonorrhoeae MS11

43.06

100

0.431
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.738

99.465

0.365
  pilF Thermus thermophilus HB27

36.299

100

0.364

Multiple sequence alignment