Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MasN3_RS05915 Genome accession   NZ_AP026966
Coordinates   1277811..1278998 (+) Length   395 a.a.
NCBI ID   WP_281912987.1    Uniprot ID   -
Organism   Massilia varians strain N-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1272811..1283998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MasN3_RS05880 (MasN3_11520) proC 1273164..1273982 (+) 819 WP_281912981.1 pyrroline-5-carboxylate reductase -
  MasN3_RS05885 (MasN3_11530) - 1274088..1274390 (-) 303 WP_281912982.1 hypothetical protein -
  MasN3_RS05890 (MasN3_11540) - 1274380..1274757 (-) 378 WP_281912983.1 phage holin family protein -
  MasN3_RS05895 (MasN3_11550) - 1274759..1275079 (-) 321 WP_209587824.1 YqjD family protein -
  MasN3_RS05900 (MasN3_11560) ubiA 1275125..1275979 (-) 855 WP_281912984.1 4-hydroxybenzoate octaprenyltransferase -
  MasN3_RS05905 (MasN3_11570) - 1276094..1276573 (+) 480 WP_281912985.1 YaiI/YqxD family protein -
  MasN3_RS05910 (MasN3_11580) - 1276725..1277666 (-) 942 WP_281912986.1 hydrogen peroxide-inducible genes activator -
  MasN3_RS05915 (MasN3_11590) pilU 1277811..1278998 (+) 1188 WP_281912987.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MasN3_RS05920 (MasN3_11600) - 1279013..1279486 (+) 474 WP_281912988.1 adenylyltransferase/cytidyltransferase family protein -
  MasN3_RS05925 (MasN3_11610) - 1279561..1280280 (-) 720 WP_281912989.1 PEP-CTERM sorting domain-containing protein -
  MasN3_RS05930 (MasN3_11620) - 1280870..1282657 (+) 1788 WP_281912990.1 S8 family serine peptidase -
  MasN3_RS05935 (MasN3_11630) - 1282757..1283593 (+) 837 WP_281912991.1 metallophosphoesterase -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 43625.77 Da        Isoelectric Point: 6.5025

>NTDB_id=99056 MasN3_RS05915 WP_281912987.1 1277811..1278998(+) (pilU) [Massilia varians strain N-3]
MSSTFGPAEAQAYIHKLLTVMHHQGGSDLFISSDFPPSMKHQGAMKPLSQQRLSGEVTRALALSLMNERQRLEFETEMEC
NFAISLPGVCRFRVNVFVQQQSVGMVVRTIASEIPNFEKLDLPDVLKDVVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNS
HSAGHIITVEDPVEYVHKNKNCLVTHREVGVDTHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNLRAVVSQRLIRTEDGKGRKAAIEILLNTPTIGEMILKGNFQNIKEIMHKS
RELGMCTFDQALYELYNKGYIGYDEAIRNADSANGLRLQIKLSGERKGPTGAGAGAAELSMAIEEEPDGDAPTTT

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=99056 MasN3_RS05915 WP_281912987.1 1277811..1278998(+) (pilU) [Massilia varians strain N-3]
ATGTCGTCGACCTTCGGCCCGGCGGAAGCCCAGGCCTACATCCACAAGCTGCTGACGGTGATGCACCACCAGGGCGGCTC
CGACCTGTTCATCTCCAGCGATTTCCCGCCCAGCATGAAGCACCAGGGCGCGATGAAGCCCCTGAGCCAGCAGCGCCTGT
CTGGCGAGGTCACGCGCGCGCTGGCCCTGTCCCTGATGAACGAGCGCCAGCGCCTCGAGTTCGAGACCGAGATGGAGTGC
AACTTCGCGATCTCGCTCCCCGGAGTATGCCGCTTTCGCGTCAACGTCTTCGTGCAGCAGCAGAGCGTGGGCATGGTGGT
GCGCACGATCGCCTCCGAGATTCCGAACTTCGAGAAGCTCGACCTGCCGGACGTGCTGAAGGACGTCGTGATGACCAAGC
GCGGCCTGGTGCTGGTGGTCGGCGGCACCGGTTCCGGCAAGTCCACCACGCTGGCGGCGATGATCGACTACCGCAACAGC
CATTCGGCGGGCCATATCATCACCGTGGAAGACCCGGTCGAGTACGTCCACAAGAACAAGAACTGCCTGGTCACGCACCG
CGAGGTGGGCGTCGACACGCACTCCTGGCACAATGCCCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGACACCGAGACCATGGAACACGCGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGGAACCCTGCAC
GCGAACAATGCCAACCAGACCCTGGACCGCATCATCAACTTCTTCCCGGAAGAGCGCCGCAACCAGCTCCTGATGGACCT
GTCGTCCAATTTGCGCGCGGTGGTGTCGCAGCGCCTGATCCGTACCGAGGACGGCAAGGGCCGCAAGGCGGCCATCGAGA
TCCTGCTGAACACGCCCACCATCGGCGAGATGATCCTGAAGGGGAACTTCCAGAACATCAAGGAGATCATGCACAAGTCG
CGCGAGCTCGGCATGTGCACCTTCGACCAGGCCTTGTACGAGCTGTACAACAAGGGCTACATCGGCTACGACGAAGCGAT
CCGCAACGCCGACTCGGCCAACGGCCTGCGCCTGCAGATCAAGCTCTCGGGCGAGCGCAAGGGGCCGACTGGTGCCGGCG
CCGGCGCGGCCGAGCTGTCGATGGCGATCGAGGAAGAACCGGACGGCGATGCCCCCACCACGACTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(124-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.34

89.367

0.539
  pilU Acinetobacter baylyi ADP1

53.955

89.62

0.484
  pilU Vibrio cholerae strain A1552

49.727

92.658

0.461
  pilT Pseudomonas aeruginosa PAK

43.62

85.316

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

85.316

0.372
  pilT Legionella pneumophila strain ERS1305867

44.377

83.291

0.37
  pilT Legionella pneumophila strain Lp02

44.377

83.291

0.37
  pilT Pseudomonas stutzeri DSM 10701

43.323

85.316

0.37
  pilT Acinetobacter baylyi ADP1

42.943

84.304

0.362

Multiple sequence alignment