Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R8635_RS10430 Genome accession   NZ_AP026924
Coordinates   2000008..2001024 (-) Length   338 a.a.
NCBI ID   WP_075218762.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700063     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1995008..2006024
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8635_RS10385 - 1995085..1995216 (-) 132 WP_000768904.1 hypothetical protein -
  R8635_RS10390 (PC0062_20280) - 1995217..1996407 (-) 1191 WP_050245357.1 acetate kinase -
  R8635_RS10395 (PC0062_20290) comYH 1996458..1997411 (-) 954 WP_050233486.1 class I SAM-dependent methyltransferase Machinery gene
  R8635_RS10400 - 1997472..1998066 (-) 595 Protein_2030 class I SAM-dependent methyltransferase -
  R8635_RS10405 (PC0062_20320) comGG/cglG 1998203..1998616 (-) 414 WP_000265620.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8635_RS10410 comGF/cglF 1998594..1999055 (-) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8635_RS10415 (PC0062_20340) comGE/cglE 1999018..1999320 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8635_RS10420 (PC0062_20350) comGD/cglD 1999283..1999687 (-) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8635_RS10425 (PC0062_20360) comGC/cglC 1999680..2000006 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  R8635_RS10430 (PC0062_20370) comGB/cglB 2000008..2001024 (-) 1017 WP_075218762.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8635_RS10435 (PC0062_20380) comGA/cglA/cilD 2000972..2001913 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  R8635_RS10440 (PC0062_20390) - 2001989..2002354 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8635_RS10445 (PC0062_20400) - 2002505..2003563 (-) 1059 WP_000649470.1 zinc-dependent alcohol dehydrogenase family protein -
  R8635_RS10450 - 2003818..2005094 (+) 1277 Protein_2040 ISL3 family transposase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38478.51 Da        Isoelectric Point: 9.6073

>NTDB_id=98305 R8635_RS10430 WP_075218762.1 2000008..2001024(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700063]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTI
ITQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=98305 R8635_RS10430 WP_075218762.1 2000008..2001024(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700063]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGCACCATT
ATCACTCAGTTATCCCTAGCTGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment