Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AAFX12_RS02685 Genome accession   NZ_CP151938
Coordinates   549661..550884 (-) Length   407 a.a.
NCBI ID   WP_064368761.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain TBV025     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544661..555884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX12_RS02650 (AAFX12_02650) rimM 544775..545323 (+) 549 WP_005379964.1 ribosome maturation factor RimM -
  AAFX12_RS02655 (AAFX12_02655) trmD 545351..546112 (+) 762 WP_064354293.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  AAFX12_RS02660 (AAFX12_02660) rplS 546154..546507 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  AAFX12_RS02665 (AAFX12_02665) yacG 546991..547185 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  AAFX12_RS02670 (AAFX12_02670) zapD 547344..548084 (-) 741 WP_005373086.1 cell division protein ZapD -
  AAFX12_RS02675 (AAFX12_02675) coaE 548112..548726 (-) 615 WP_064354292.1 dephospho-CoA kinase -
  AAFX12_RS02680 (AAFX12_02680) pilD 548727..549596 (-) 870 WP_064354291.1 prepilin peptidase Machinery gene
  AAFX12_RS02685 (AAFX12_02685) pilC 549661..550884 (-) 1224 WP_064368761.1 type II secretion system F family protein Machinery gene
  AAFX12_RS02690 (AAFX12_02690) pilB 550908..552593 (-) 1686 WP_005379975.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAFX12_RS02695 (AAFX12_02695) - 552593..553051 (-) 459 WP_064368760.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AAFX12_RS02700 (AAFX12_02700) nadC 553313..554200 (-) 888 WP_017820396.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAFX12_RS02705 (AAFX12_02705) ampD 554293..554844 (+) 552 WP_005386614.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45312.48 Da        Isoelectric Point: 10.3446

>NTDB_id=981356 AAFX12_RS02685 WP_064368761.1 549661..550884(-) (pilC) [Vibrio alginolyticus strain TBV025]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVREHLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSMSLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=981356 AAFX12_RS02685 WP_064368761.1 549661..550884(-) (pilC) [Vibrio alginolyticus strain TBV025]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCTGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACAGAAATTGAAGTACGCGAGCATCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TTTCGTTTCTCACTAAACTCAGCCATCGCGTAAAAGGCAGAGACATCACGGTGTTTACCCGCCAAATTTCGACGATGTTG
ATAACCGGTGTGCCCTTGGTTCAGGCTTTAAAATTGGTATCAGAGAATCACAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGGACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGATCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCAGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCTATGGTAGTGCTAGTCGCGTTAGGTGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGTTTTGGTGCTGATTTACCTTGGTTTACCAGGC
AAGTATTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATGAGTTTATTCATCTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAATCGCTCTGTGCTTAAATTTCCTGTCCTCGGGCCCGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACGAGCTTTACAGCGGGCATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTTGCCATGCGTAACTGTAATGTGTTCCCTGAATTAGTGTTACAAATGGTCATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGATAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCGCTAATTATCGTTTTTTTAGGCATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.978

100

0.87
  pilC Vibrio cholerae strain A1552

73.775

100

0.74
  pilC Acinetobacter baylyi ADP1

42.75

98.28

0.42
  pilC Acinetobacter baumannii D1279779

41.191

99.017

0.408
  pilC Legionella pneumophila strain ERS1305867

40.295

100

0.403
  pilG Neisseria gonorrhoeae MS11

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilG Neisseria meningitidis 44/76-A

39.901

99.754

0.398

Multiple sequence alignment