Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAFX12_RS02690 Genome accession   NZ_CP151938
Coordinates   550908..552593 (-) Length   561 a.a.
NCBI ID   WP_005379975.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain TBV025     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 545908..557593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX12_RS02660 (AAFX12_02660) rplS 546154..546507 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  AAFX12_RS02665 (AAFX12_02665) yacG 546991..547185 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  AAFX12_RS02670 (AAFX12_02670) zapD 547344..548084 (-) 741 WP_005373086.1 cell division protein ZapD -
  AAFX12_RS02675 (AAFX12_02675) coaE 548112..548726 (-) 615 WP_064354292.1 dephospho-CoA kinase -
  AAFX12_RS02680 (AAFX12_02680) pilD 548727..549596 (-) 870 WP_064354291.1 prepilin peptidase Machinery gene
  AAFX12_RS02685 (AAFX12_02685) pilC 549661..550884 (-) 1224 WP_064368761.1 type II secretion system F family protein Machinery gene
  AAFX12_RS02690 (AAFX12_02690) pilB 550908..552593 (-) 1686 WP_005379975.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAFX12_RS02695 (AAFX12_02695) - 552593..553051 (-) 459 WP_064368760.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AAFX12_RS02700 (AAFX12_02700) nadC 553313..554200 (-) 888 WP_017820396.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAFX12_RS02705 (AAFX12_02705) ampD 554293..554844 (+) 552 WP_005386614.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AAFX12_RS02710 (AAFX12_02710) pdhR 555250..556017 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62120.96 Da        Isoelectric Point: 5.5200

>NTDB_id=981357 AAFX12_RS02690 WP_005379975.1 550908..552593(-) (pilB) [Vibrio alginolyticus strain TBV025]
MLTNLSTVLRQAGILTFSQEELLLEQAKASGISMPEALLNSGFFQSHELTEHLSSVFGLSCASLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=981357 AAFX12_RS02690 WP_005379975.1 550908..552593(-) (pilB) [Vibrio alginolyticus strain TBV025]
ATGCTAACCAACCTCTCAACAGTTCTTCGCCAAGCAGGGATACTGACTTTTTCCCAAGAAGAACTGTTACTTGAGCAAGC
TAAAGCTTCTGGTATATCAATGCCGGAGGCTTTGCTCAATTCTGGCTTTTTTCAATCTCATGAATTAACGGAGCACCTAA
GCTCTGTTTTCGGGCTTTCTTGCGCTTCTCTCAATCAATACGAATACGCTTCGTTATGCCAAACACTAGGTTTGCGAGAT
TTGATCACTCGGCACAACGCTCTGCCGCTTAATCGCACCTCATCAACTTTGATTCTCGCGGTTGCCGATCCAACCAATCT
TCAAGCCGAAGACGACTTTCGTTTTGCAACAGGCTTGCAAGTCGAACTTGTTCTGGCTGATTTTCGCGAACTGACCGCTG
CGATTCGGCGCTTATATGGTCGTTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATAAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGCGTGCGCC
TACGTTGCGATGGCATCTTAATCGAAACACAGCAGCCGCCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCGAT
TGATATGCGTGTATCCACGCTACCTACTCTATTCGGAGAGAAAATTGTATTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACTGGGAGCGGGAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTTCTTCGTCAAGACCCCGATGTGGTCATGGTCGGTGAGATTCGTGACTTAGACACT
GCGGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTGCATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGTAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGAATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

93.405

100

0.934
  pilB Vibrio parahaemolyticus RIMD 2210633

93.226

100

0.932
  pilB Vibrio cholerae strain A1552

74.021

100

0.742
  pilB Acinetobacter baumannii D1279779

51.731

92.692

0.48
  pilB Legionella pneumophila strain ERS1305867

48.987

96.791

0.474
  pilB Acinetobacter baylyi ADP1

48.708

96.613

0.471
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

36.594

100

0.387
  pilF Thermus thermophilus HB27

36.477

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.315

99.643

0.362

Multiple sequence alignment