Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   WLF14_RS04350 Genome accession   NZ_CP151816
Coordinates   959332..960540 (-) Length   402 a.a.
NCBI ID   WP_069075201.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain IMGN2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 954332..965540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WLF14_RS04310 (WLF14_04310) - 955217..955390 (-) 174 WP_024073379.1 DUF3094 family protein -
  WLF14_RS04315 (WLF14_04315) - 955480..956109 (-) 630 WP_017845475.1 DUF1780 domain-containing protein -
  WLF14_RS04320 (WLF14_04320) - 956210..956677 (+) 468 WP_069075203.1 FAD/FMN-containing dehydrogenase -
  WLF14_RS04325 (WLF14_04325) - 956674..957363 (+) 690 WP_024073381.1 energy-coupling factor ABC transporter permease -
  WLF14_RS04330 (WLF14_04330) - 957440..957655 (+) 216 WP_003171680.1 hypothetical protein -
  WLF14_RS04335 (WLF14_04335) yacG 957652..957852 (-) 201 WP_017845472.1 DNA gyrase inhibitor YacG -
  WLF14_RS04340 (WLF14_04340) coaE 957849..958472 (-) 624 WP_024073382.1 dephospho-CoA kinase -
  WLF14_RS04345 (WLF14_04345) pilD 958469..959335 (-) 867 WP_069075202.1 A24 family peptidase Machinery gene
  WLF14_RS04350 (WLF14_04350) pilC 959332..960540 (-) 1209 WP_069075201.1 type II secretion system F family protein Machinery gene
  WLF14_RS04355 (WLF14_04355) - 960742..961155 (+) 414 WP_024073385.1 pilin -
  WLF14_RS04360 (WLF14_04360) - 961190..962599 (+) 1410 WP_069075200.1 O-antigen ligase family protein -
  WLF14_RS04370 (WLF14_04370) - 962862..963167 (+) 306 WP_024073387.1 DUF6388 family protein -
  WLF14_RS04375 (WLF14_04375) - 963192..963281 (+) 90 Protein_851 outer membrane lipoprotein carrier protein LolA -
  WLF14_RS04380 (WLF14_04380) nadC 963289..964137 (-) 849 WP_150054127.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43306.60 Da        Isoelectric Point: 10.0668

>NTDB_id=980995 WLF14_RS04350 WP_069075201.1 959332..960540(-) (pilC) [Pseudomonas fluorescens strain IMGN2]
MDNASRTYAWEGTNRQGHRVSGHTTGHNLALIKAKLRQQGIRPSRVRRQFPGLACLAAPIKPTDIALLTRQLATLLKAGI
PLLQAFDIISDGFESRSMQELLKGLKQDIAAGTALATALRKQPRYFDDLYCNLIAAGEQAGALETLLERIATHLEKSEQL
KARIKKAMTYPIAVLVVASLVSSILLIHVVPQFQNLFAGVDGQLPGFTLHVIGLSTFMQQAWWALALGLGAGFFALRHAY
RVAPGFRQGLDTGLLKAPLAGTLLKKSAVARYARTLSTTFAAGVPLVQALDSVAGAVGNGPYKQAIKHMRHEVSTGMQLN
QSMAASGLFPGMAIQMTAIGEESGTLERMLEKVANHYESEVDNLVDNLTSLMEPLIMVVLGGIVGALVIAMYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=980995 WLF14_RS04350 WP_069075201.1 959332..960540(-) (pilC) [Pseudomonas fluorescens strain IMGN2]
ATGGACAACGCTTCAAGGACCTACGCGTGGGAAGGCACCAACCGCCAGGGGCACAGGGTGTCCGGGCACACCACGGGCCA
CAACCTCGCCCTGATCAAGGCCAAGCTGCGCCAGCAAGGGATCCGCCCCAGCCGTGTGCGCAGGCAATTCCCCGGCCTGG
CCTGCCTCGCCGCGCCGATCAAACCCACTGACATCGCCCTGCTCACCCGCCAGTTGGCGACCTTGCTGAAAGCCGGCATT
CCTCTGCTGCAAGCCTTCGATATCATCAGCGACGGCTTCGAGAGCCGGTCGATGCAGGAACTGCTCAAGGGGTTGAAGCA
GGACATCGCCGCCGGCACCGCACTGGCGACCGCGCTGCGCAAACAGCCACGCTATTTCGATGACCTGTACTGCAACCTGA
TTGCCGCCGGCGAACAGGCCGGGGCATTGGAAACGCTGCTGGAACGCATTGCCACTCACCTGGAAAAGAGTGAGCAGCTA
AAGGCGCGAATCAAGAAAGCCATGACCTACCCGATCGCCGTCCTGGTGGTGGCGAGCCTGGTCAGCAGCATCCTGCTGAT
CCACGTGGTGCCGCAGTTTCAGAACCTGTTCGCCGGAGTCGACGGCCAGTTGCCCGGTTTTACCCTGCACGTCATCGGCC
TGTCCACGTTCATGCAGCAAGCCTGGTGGGCTCTGGCGCTTGGCCTCGGGGCCGGTTTTTTCGCGCTGCGCCACGCCTAT
CGCGTAGCCCCTGGCTTTCGTCAGGGGCTGGATACGGGTTTGTTGAAAGCCCCCTTGGCAGGCACACTGCTGAAGAAATC
GGCCGTGGCACGCTATGCCCGCACGCTTTCCACCACGTTCGCGGCTGGGGTGCCGCTGGTACAGGCATTGGATTCAGTGG
CAGGTGCCGTCGGCAACGGGCCGTACAAGCAGGCGATCAAACATATGCGTCATGAGGTATCCACAGGCATGCAGTTGAAC
CAATCCATGGCCGCCAGCGGTCTCTTTCCAGGCATGGCAATTCAGATGACGGCCATCGGCGAGGAGTCGGGCACACTCGA
GCGCATGCTGGAAAAAGTCGCGAACCACTATGAATCGGAAGTCGACAACTTGGTCGACAACCTCACCAGCCTGATGGAGC
CGCTGATAATGGTGGTGCTGGGGGGCATTGTCGGGGCGCTGGTAATTGCGATGTACCTGCCGGTCTTCCAACTCGGTACA
GCGTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.071

98.507

0.562
  pilC Acinetobacter baylyi ADP1

52.261

99.005

0.517
  pilC Acinetobacter baumannii D1279779

49.747

98.507

0.49
  pilC Legionella pneumophila strain ERS1305867

48.99

98.507

0.483
  pilC Vibrio cholerae strain A1552

40.1

99.254

0.398
  pilG Neisseria gonorrhoeae MS11

38.791

98.756

0.383
  pilC Vibrio campbellii strain DS40M4

38.191

99.005

0.378
  pilG Neisseria meningitidis 44/76-A

38.287

98.756

0.378
  pilC Thermus thermophilus HB27

37.656

99.751

0.376

Multiple sequence alignment