Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AABM54_RS15655 Genome accession   NZ_CP150918
Coordinates   3477567..3478676 (-) Length   369 a.a.
NCBI ID   WP_347900891.1    Uniprot ID   -
Organism   Pseudomonas purpurea strain DGS26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3472567..3483676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM54_RS15650 - 3473676..3477416 (-) 3741 WP_347900890.1 hypothetical protein -
  AABM54_RS15655 pilU 3477567..3478676 (-) 1110 WP_347900891.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AABM54_RS15660 - 3478807..3481344 (+) 2538 WP_347900892.1 PAS domain-containing protein -
  AABM54_RS15665 - 3481397..3481672 (+) 276 WP_166589508.1 peptidylprolyl isomerase -
  AABM54_RS15670 - 3481796..3482719 (-) 924 WP_347900893.1 sugar kinase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41317.48 Da        Isoelectric Point: 7.0912

>NTDB_id=973779 AABM54_RS15655 WP_347900891.1 3477567..3478676(-) (pilU) [Pseudomonas purpurea strain DGS26]
MEIHSLLRMLASQDGSDLYLSTGAPPSGRFNGALRPLTREAFKTGEIAAIAASIMDAEQRAEFDRELEMNLAISLVGIGR
FRINIFKQRNDVSIVARNIKLEIPRFEDLKLPRVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVMSTLHAHNANQALDRI
INFFPEERRPQLLIDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIADLIRRNELGELKNIIEKSAEQGMQTFDGEL
YKLVVEGVVSESEALKHADSVNNLRLRLKLDVESAAVKKLPPGEWGLIE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=973779 AABM54_RS15655 WP_347900891.1 3477567..3478676(-) (pilU) [Pseudomonas purpurea strain DGS26]
ATGGAAATTCACTCGTTGTTGAGAATGCTGGCCAGTCAGGATGGTTCCGATCTGTACCTGTCCACCGGGGCACCGCCCAG
CGGTCGTTTCAATGGGGCTCTTCGGCCGCTGACCCGTGAAGCGTTCAAGACCGGGGAGATCGCCGCCATCGCAGCGAGCA
TTATGGACGCCGAACAGCGCGCCGAGTTCGACCGGGAGCTGGAAATGAACCTGGCGATTTCCCTGGTCGGCATCGGGCGG
TTTCGGATCAATATCTTCAAACAGCGCAATGACGTGTCCATCGTCGCCCGCAACATCAAACTTGAGATTCCACGTTTCGA
AGACCTCAAATTGCCGCGGGTGCTGCTTGAAACCGTCATGCTCAAGCAGGGCCTGATGCTGTTTGTGGGGGCCACCGATT
CTGGCAAATCAACGTCGCTGGCGGCACTGATCGATTACCGCAACCGCCACAGCAGTGGTCACATCATCACCATCGAAGAC
CCGGTGGAGTACATCCACCGACACCAGAAGTCGATCATCAACCAGCGCGAAGTCGGGGTCGACACCCGAAGCTTTCATGC
CGCGCTGAAAAACACCCTGCGCCAGGCACCGGATGTGGTGCTGATCGGCGAAATCCGCGACCGCGAAACCATGGAACACG
CCCTGGCGTTTGCCGACACCGGGCATCTGGTGATGTCTACCCTGCATGCCCACAACGCCAACCAGGCGCTGGATCGCATC
ATCAATTTTTTCCCCGAAGAGCGTCGGCCGCAGTTGCTGATCGACCTGGGCAACAACCTCAAGGCGTTCGTCTCGCAGCG
TTTGGTCCGTACCCGCGACGGTCAGCGTCGCGCCGCCGTGGAAGTGATGCTGGGCACGCCGACCATCGCCGACCTGATCC
GGCGCAACGAGTTGGGCGAACTCAAGAACATCATTGAAAAGTCTGCGGAGCAGGGTATGCAGACCTTCGATGGCGAGCTT
TACAAGTTGGTTGTGGAGGGGGTGGTCAGTGAGAGTGAAGCGTTGAAACATGCGGACTCCGTTAATAATTTGAGGCTGCG
GCTGAAACTTGATGTCGAGTCGGCAGCGGTTAAGAAACTACCTCCGGGGGAGTGGGGCTTGATCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.056

96.206

0.539
  pilU Acinetobacter baylyi ADP1

51.966

96.477

0.501
  pilU Vibrio cholerae strain A1552

52

94.851

0.493
  pilT Vibrio cholerae strain A1552

41.742

90.244

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

90.244

0.377
  pilT Pseudomonas stutzeri DSM 10701

39.535

93.225

0.369
  pilT Pseudomonas aeruginosa PAK

38.953

93.225

0.363
  pilT Legionella pneumophila strain Lp02

39.82

90.515

0.36
  pilT Legionella pneumophila strain ERS1305867

39.82

90.515

0.36

Multiple sequence alignment