Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AABM56_RS18480 Genome accession   NZ_CP150916
Coordinates   3983065..3984759 (-) Length   564 a.a.
NCBI ID   WP_347883608.1    Uniprot ID   -
Organism   Pseudomonas aestiva strain DGS32     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3978065..3989759
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM56_RS18455 - 3979453..3979950 (+) 498 WP_347883604.1 hypothetical protein -
  AABM56_RS18460 yacG 3979963..3980160 (-) 198 WP_007160931.1 DNA gyrase inhibitor YacG -
  AABM56_RS18465 coaE 3980157..3980768 (-) 612 WP_347883605.1 dephospho-CoA kinase -
  AABM56_RS18470 pilD 3980765..3981637 (-) 873 WP_347883606.1 A24 family peptidase Machinery gene
  AABM56_RS18475 pilC 3981637..3982854 (-) 1218 WP_347883607.1 type II secretion system F family protein Machinery gene
  AABM56_RS18480 pilB 3983065..3984759 (-) 1695 WP_347883608.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AABM56_RS18485 comP 3984972..3985406 (+) 435 WP_347883609.1 pilin Machinery gene
  AABM56_RS18490 - 3985842..3987167 (+) 1326 WP_347884133.1 Wzy polymerase domain-containing protein -
  AABM56_RS18495 - 3987155..3988066 (+) 912 WP_347883610.1 glycosyltransferase family 2 protein -
  AABM56_RS18500 - 3988063..3988959 (+) 897 WP_347883611.1 glycosyltransferase -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62449.81 Da        Isoelectric Point: 5.7815

>NTDB_id=973728 AABM56_RS18480 WP_347883608.1 3983065..3984759(-) (pilB) [Pseudomonas aestiva strain DGS32]
MNETVMLSGLARQLVDAQLLAEAAAQQAVVKAKQAKVPLVTYLVQQRLVQSRPLAELAAQEFGVPFLDLAAIDKEQLPKD
IVNEKLMQQHHFLPLSKRGNKLFIALSDPTNQQAINDVSFGSKLAVEVVLVEDDKLHQAIERYLDTGTGGLELSDLDLDL
EAEGGERPQEAVAGTDADDAPVVKFVNKMLLDAIKKGSSDLHFEPYEKMYRVRFRTDGVLHEAARPPVQLVSRISARLKV
MSNMDISERRKPQDGRVKLKVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEDVQKELYLQALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTEDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNLATSVNLIIAQRLARKLCNKCKRVHEVPREALLAEGFREEE
IGSFTLYQPIGCEDCNAGYRGRVGIYEVVKVTPALQQMIMEEGNSIEIAARMRKEGFNDLRRSGLVKAMQGITSLEEINR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=973728 AABM56_RS18480 WP_347883608.1 3983065..3984759(-) (pilB) [Pseudomonas aestiva strain DGS32]
ATGAACGAGACGGTGATGCTGTCGGGACTGGCGCGGCAACTGGTGGATGCGCAGTTGCTGGCGGAGGCGGCTGCCCAGCA
GGCGGTGGTCAAGGCCAAGCAAGCCAAGGTGCCGCTGGTCACCTATCTCGTGCAGCAGCGATTGGTGCAGAGCCGGCCCT
TGGCGGAGCTTGCTGCCCAGGAGTTCGGGGTGCCCTTTCTCGATCTGGCGGCTATCGACAAGGAGCAGCTGCCCAAGGAC
ATCGTCAACGAGAAGCTGATGCAGCAGCATCATTTCCTGCCTTTGAGCAAAAGAGGCAACAAGCTTTTCATCGCGCTTTC
CGATCCTACCAACCAGCAGGCCATCAATGATGTTTCCTTTGGCAGCAAGCTGGCGGTGGAGGTCGTGCTGGTCGAAGATG
ACAAGCTACATCAGGCCATCGAGCGATACCTCGATACGGGAACCGGTGGGCTGGAGTTGTCCGATCTCGATCTCGATCTG
GAAGCTGAGGGAGGAGAACGGCCTCAGGAAGCCGTTGCGGGTACCGACGCCGACGATGCGCCCGTGGTGAAGTTCGTCAA
CAAGATGCTGCTCGATGCCATCAAGAAGGGGTCCTCCGACCTGCACTTCGAACCCTATGAAAAGATGTACCGGGTGCGCT
TCCGTACCGATGGCGTCTTGCACGAGGCGGCGCGGCCGCCGGTGCAGCTGGTGTCGCGGATCTCGGCGCGGCTCAAGGTG
ATGTCCAATATGGATATCTCCGAGCGGCGCAAACCCCAGGATGGCCGGGTCAAGCTAAAGGTCTCCAAGACCAAGTCCAT
CGACTTCCGGGTGAACACCCTGCCCACCCTCTGGGGCGAGAAGATCGTGATGCGGATTCTGGATTCCTCCAGCGCCCAGA
TGGGGATCGATGCCCTGGGCTATGAGGATGTGCAGAAGGAGCTCTATCTGCAGGCACTCAAGCAGCCGCAGGGGATGATC
CTGGTGACCGGACCAACCGGTTCGGGGAAGACCGTATCCCTCTATACCGGCCTTAACATCCTCAATACCGAAGACATCAA
TATTTCCACGGCGGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGGCAGGGACTCG
ACTTCTCCCAGGCACTACGCGCCTTCCTGCGTCAGGATCCTGACGTGATCATGGTGGGTGAGATCCGCGATCTGGAAACG
GCAGAGATCGCCATCAAGGCGGCCCAGACCGGTCACATGGTGATGTCGACCCTGCACACCAACAGCGCGGCGGAAACCTT
GACGCGTCTACGTAACATGGGGGTGCCCGCCTTCAATCTGGCGACCTCGGTCAATCTGATCATCGCCCAGCGCTTGGCGC
GCAAGCTGTGCAACAAGTGCAAGAGAGTTCATGAGGTGCCGCGTGAGGCCCTATTGGCCGAAGGCTTCCGCGAGGAAGAG
ATCGGCAGTTTTACCCTTTACCAGCCCATCGGCTGCGAGGACTGCAATGCGGGTTATCGCGGGCGAGTGGGGATCTATGA
GGTGGTGAAGGTCACGCCAGCGTTGCAGCAAATGATCATGGAGGAGGGCAACTCCATCGAGATCGCGGCGCGGATGCGCA
AAGAGGGCTTCAATGATCTGCGGCGGTCAGGGCTGGTGAAGGCGATGCAGGGGATTACCAGTCTGGAAGAGATCAATCGG
GTGACTAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.893

99.468

0.596
  pilB Acinetobacter baylyi ADP1

59.715

99.468

0.594
  pilB Legionella pneumophila strain ERS1305867

54.073

100

0.553
  pilB Vibrio cholerae strain A1552

50.088

100

0.502
  pilB Vibrio campbellii strain DS40M4

49.734

99.823

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

49.376

99.468

0.491
  pilF Neisseria gonorrhoeae MS11

49.023

99.823

0.489
  pilF Thermus thermophilus HB27

37.143

99.291

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.048

93.085

0.363

Multiple sequence alignment