Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AABD45_RS14875 Genome accession   NZ_CP150831
Coordinates   3190228..3192018 (+) Length   596 a.a.
NCBI ID   WP_045595641.1    Uniprot ID   -
Organism   Vibrio vulnificus strain Per1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3185228..3197018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABD45_RS14855 (AABD45_15390) pilM 3187542..3188549 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  AABD45_RS14860 (AABD45_15395) pilN 3188539..3189129 (+) 591 WP_038940289.1 PilN domain-containing protein Machinery gene
  AABD45_RS14865 (AABD45_15400) pilO 3189122..3189712 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  AABD45_RS14870 (AABD45_15405) pilP 3189702..3190214 (+) 513 WP_038940290.1 pilus assembly protein PilP Machinery gene
  AABD45_RS14875 (AABD45_15410) pilQ 3190228..3192018 (+) 1791 WP_045595641.1 type IV pilus secretin PilQ Machinery gene
  AABD45_RS14880 (AABD45_15415) aroK 3192220..3192738 (+) 519 WP_011079357.1 shikimate kinase AroK -
  AABD45_RS14885 (AABD45_15420) aroB 3192771..3193862 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -
  AABD45_RS14890 (AABD45_15425) - 3193913..3195409 (+) 1497 WP_130197698.1 AAA family ATPase -
  AABD45_RS14895 (AABD45_15430) - 3195489..3196316 (+) 828 WP_017419662.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 596 a.a.        Molecular weight: 64735.79 Da        Isoelectric Point: 4.5077

>NTDB_id=972744 AABD45_RS14875 WP_045595641.1 3190228..3192018(+) (pilQ) [Vibrio vulnificus strain Per1]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGSGDSGSGGSGDEGGSNSID
DFLNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTV
SFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKV
PLLGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1791 bp        

>NTDB_id=972744 AABD45_RS14875 WP_045595641.1 3190228..3192018(+) (pilQ) [Vibrio vulnificus strain Per1]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACTTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAATGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACCGTGGTGGATTTGAAACCAGTGGCAGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTTAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTTTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGCCTTGTTGCGAATGTCAGCGGTGAATTTCGTCACGACTATCGTCTTT
CTGGACGCTATCTCGAGGTGACAATTTCTCGCATCAAATCGGAGCAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGCTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCTGTCACTGGTAATGTGACGCTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGACATCA
TTCTTCAAGTGAAAGGTCTAGACAAACGTGTTGAAGGCAATGTTGTGCTCATTGCTCCTCAAGATGAACTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAACGTCTGGCGGAAGAGCTTGGCTCCCTTAAATCGGAAATCATCAAAGTCAACTTTGC
TAAAGCGTCAGACATTGCTGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCGCGCATTGTCACGGTGAATGAAGGCAATCTTGATGAGCTTGGTGTCCGTTG
GGGGTTCACCTCCATCAACGGCAGCACAACCGTCGGCGGTTCAATCGAAAACAACCTAGCAACCATTGGTCTCTACAATG
CAGGCAGTGGGAGTGGCAGTGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGTGATGAAGGCGGGTCGAACTCCATTGAT
GATTTCCTCAATGTCAATCTTGCTGCGACTTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGA
TTTGTTGTTGGATCTCGAGCTATCAGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAA
CCAATAAGAAGCCTGCTTATATCGAGCAAGGTACAGAGATTCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTT
TCCTTTAAGAAAGCGGTGCTTAGCCTGAAAGTAACCCCTCAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGT
TACACAAGATAGACCTGGCGAAATTGTCAAAACCGGTACTGGGGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACGC
AAGTCTTGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGCATAACCAATACCACGGACAAGGTT
CCTCTGTTAGGCGATTTACCCTTATTGGGTGCGCTTTTCCGCCGTAGCTATGAAAACGTAGGCAAACGTGAATTGCTGAT
ATTTGTCACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(436-594)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.534

99.329

0.77
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

73.818

99.329

0.733
  pilQ Vibrio cholerae strain A1552

73.818

99.329

0.733
  pilQ Legionella pneumophila strain Lp02

41.281

94.295

0.389
  pilQ Legionella pneumophila strain ERS1305867

41.281

94.295

0.389
  pilQ Pseudomonas aeruginosa PAK

38.302

96.812

0.371
  comQ Acinetobacter baylyi ADP1

36.7

99.664

0.366

Multiple sequence alignment