Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AABD45_RS00690 Genome accession   NZ_CP150831
Coordinates   119628..121316 (-) Length   562 a.a.
NCBI ID   WP_225490803.1    Uniprot ID   -
Organism   Vibrio vulnificus strain Per1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114628..126316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABD45_RS00660 (AABD45_00660) rplS 115174..115527 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  AABD45_RS00665 (AABD45_00665) yacG 115751..115945 (-) 195 WP_038941426.1 DNA gyrase inhibitor YacG -
  AABD45_RS00670 (AABD45_00670) zapD 116008..116748 (-) 741 WP_017421189.1 cell division protein ZapD -
  AABD45_RS00675 (AABD45_00675) coaE 116781..117389 (-) 609 WP_225490802.1 dephospho-CoA kinase -
  AABD45_RS00680 (AABD45_00680) pilD 117391..118260 (-) 870 WP_130199940.1 A24 family peptidase Machinery gene
  AABD45_RS00685 (AABD45_00685) pilC 118354..119580 (-) 1227 WP_406849130.1 type II secretion system F family protein Machinery gene
  AABD45_RS00690 (AABD45_00690) pilB 119628..121316 (-) 1689 WP_225490803.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AABD45_RS00695 (AABD45_00695) pilA 121323..121787 (-) 465 WP_072603236.1 pilin Machinery gene
  AABD45_RS00700 (AABD45_00700) nadC 122010..122897 (-) 888 WP_017421195.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AABD45_RS00705 (AABD45_00705) ampD 122990..123562 (+) 573 WP_225490804.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AABD45_RS00710 (AABD45_00710) pdhR 123951..124718 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62430.48 Da        Isoelectric Point: 5.8447

>NTDB_id=972714 AABD45_RS00690 WP_225490803.1 119628..121316(-) (pilB) [Vibrio vulnificus strain Per1]
MQTNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQADELTERLAAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFNEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=972714 AABD45_RS00690 WP_225490803.1 119628..121316(-) (pilB) [Vibrio vulnificus strain Per1]
ATGCAAACCAACCTTGCCACAATTCTTCGTCAGGCCAATCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGCTTTTTTCAAGCTGATGAGCTAACCGAGCGCCTTG
CCGCGATTTTTGGCTTACCACGCACAGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGCTTACGTGAG
CTAATCACCCGTCACCAAGCACTGCCGCTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAAGTGGAGATTGTTTTAGCCGATTGCCAAGAACTGCAAGCAG
CGATTCGTAGACTCTATGGAAAGAGCATAAAACAGAGCCAAAGCTCTGGGTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATCTAGTCGAGGTTTCAGATGACGAAGTCGAGTCCATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTAT
CAACCAAATTCTGCTTGATGCGGTACGCAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTAC
GATTGCGTTGCGATGGTATTTTGGTAGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAA
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTACGCCTCAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACTTTACCCACGCTGTTTGGTGAAAAAATTGTGCTGCGACTTCTCGACAGCTCGAGTAGCA
GCTTGGATATCGACAAGTTAGGCTACAACCCACAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGAATG
ATTTTAATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCTCTGTACACGGGCTTGAGTTTGCTCAACCGACCTGAAGT
GAATATCTCCACCGCGGAAGACCCGGTCGAAATTAACCTCCCAGGTATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCCGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTACATACAAACTCGGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACCTGGCTTCATCCTTAAGTTTGATCATTGCTCAGCGTCTTG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCGCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCCGATAAACAGGGTTGCCATCATTGTACTCATGGTTACTCAGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTAATGAGCAGCTAGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTCGCTCGGCAAAAAG
GCATGATGACACTCAAAGACTCTGGCCTCGAGAAACTCAAACAAGGCATCACTAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.335

100

0.763
  pilB Vibrio parahaemolyticus RIMD 2210633

75.445

100

0.754
  pilB Vibrio cholerae strain A1552

74.6

100

0.747
  pilB Legionella pneumophila strain ERS1305867

51.803

93.772

0.486
  pilB Acinetobacter baumannii D1279779

51.445

92.349

0.475
  pilB Acinetobacter baylyi ADP1

50.881

90.925

0.463
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.608

99.466

0.404
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.375

Multiple sequence alignment