Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   WKQ99_RS05185 Genome accession   NZ_CP149965
Coordinates   1102568..1103785 (-) Length   405 a.a.
NCBI ID   WP_024014342.1    Uniprot ID   V8R166
Organism   Pseudomonas atacamensis strain EMP42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1097568..1108785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKQ99_RS05145 (WKQ99_05145) - 1098406..1098579 (-) 174 WP_007909875.1 DUF3094 domain-containing protein -
  WKQ99_RS05150 (WKQ99_05150) - 1098631..1099158 (-) 528 WP_256811015.1 MOSC domain-containing protein -
  WKQ99_RS05155 (WKQ99_05155) - 1099155..1099781 (-) 627 WP_007909887.1 DUF1780 domain-containing protein -
  WKQ99_RS05160 (WKQ99_05160) - 1099899..1100588 (+) 690 WP_016773147.1 energy-coupling factor ABC transporter permease -
  WKQ99_RS05165 (WKQ99_05165) - 1100654..1100869 (+) 216 WP_016773148.1 hypothetical protein -
  WKQ99_RS05170 (WKQ99_05170) yacG 1100877..1101077 (-) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  WKQ99_RS05175 (WKQ99_05175) coaE 1101074..1101697 (-) 624 WP_339101036.1 dephospho-CoA kinase -
  WKQ99_RS05180 (WKQ99_05180) pilD 1101694..1102566 (-) 873 WP_339101037.1 A24 family peptidase Machinery gene
  WKQ99_RS05185 (WKQ99_05185) pilC 1102568..1103785 (-) 1218 WP_024014342.1 type II secretion system F family protein Machinery gene
  WKQ99_RS05190 (WKQ99_05190) pilB 1103788..1105488 (-) 1701 WP_073475466.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WKQ99_RS05195 (WKQ99_05195) - 1105715..1106152 (+) 438 WP_073475464.1 pilin -
  WKQ99_RS05200 (WKQ99_05200) - 1106451..1106804 (+) 354 WP_016773155.1 BON domain-containing protein -
  WKQ99_RS05205 (WKQ99_05205) - 1107067..1107366 (-) 300 WP_273525934.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43995.68 Da        Isoelectric Point: 10.1214

>NTDB_id=962368 WKQ99_RS05185 WP_024014342.1 1102568..1103785(-) (pilC) [Pseudomonas atacamensis strain EMP42]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSLGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=962368 WKQ99_RS05185 WP_024014342.1 1102568..1103785(-) (pilC) [Pseudomonas atacamensis strain EMP42]
ATGGCGGTCAAGGCAGCGAAAGTCAGCGTTTACGCCTGGGAAGGCACCGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGGCAGAACCCCGCACTGATCAAGGCGCAGCTGCGCAAACAAGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CTTCCCTGCTGAGCTTGGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCAACGATGATGAAG
GCCGGTGTACCGCTGTTGCAGTCCTTCGACATCATCGGCGAGGGCTTCGAAAACCCGGCCATGCGCAAGCTGGTCGACGA
GGTCAAACAGGAGGTCGCCGCCGGTAACAGCTTCGCCACGGCCCTGCGCAAGAAGCCGCAATACTTCGACGAGTTGTACT
GCAACTTGGTCGACGCCGGCGAACAGTCTGGTGCCCTCGACACTTTGCTCGAACGGGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCGGCGGTGGTGACCGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTTTCCGGGTTCGGCGCCGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCGATTCTGTTTGCACTGGTGGCAGCGTTTTTCGGCACTCGC
CACGCGTTGAAGACTTCGCAGGCCCTGCGCGACCGGCGCGATGCCTGGCTGCTGAAGCTGCCGCTGGTAGGCACTTTGAT
GTACAAGTCCGCCGTCGCCCGCTTTGCCCGCACCCTGTCGACCACTTTCGCCGCCGGGGTGCCATTGGTGGAAGCGCTGG
ATTCGGTCGCCGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCGGTGCTGCGGGTGCGGCAGGACGTCTCGACCGGCATG
CAGCTGAATTTTTCGATGCGCAGCACCGGTGTGTTTCCGAACATGGCGGTGCAGATGACCGCCATTGGCGAGGAGTCGGG
CGCGCTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8R166

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

52.75

98.765

0.521
  pilC Vibrio cholerae strain A1552

44.949

97.778

0.44
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.626

100

0.417
  pilC Thermus thermophilus HB27

39.85

98.519

0.393