Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WKQ99_RS05190 Genome accession   NZ_CP149965
Coordinates   1103788..1105488 (-) Length   566 a.a.
NCBI ID   WP_073475466.1    Uniprot ID   A0AAU7ETA2
Organism   Pseudomonas atacamensis strain EMP42     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1098788..1110488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKQ99_RS05155 (WKQ99_05155) - 1099155..1099781 (-) 627 WP_007909887.1 DUF1780 domain-containing protein -
  WKQ99_RS05160 (WKQ99_05160) - 1099899..1100588 (+) 690 WP_016773147.1 energy-coupling factor ABC transporter permease -
  WKQ99_RS05165 (WKQ99_05165) - 1100654..1100869 (+) 216 WP_016773148.1 hypothetical protein -
  WKQ99_RS05170 (WKQ99_05170) yacG 1100877..1101077 (-) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  WKQ99_RS05175 (WKQ99_05175) coaE 1101074..1101697 (-) 624 WP_339101036.1 dephospho-CoA kinase -
  WKQ99_RS05180 (WKQ99_05180) pilD 1101694..1102566 (-) 873 WP_339101037.1 A24 family peptidase Machinery gene
  WKQ99_RS05185 (WKQ99_05185) pilC 1102568..1103785 (-) 1218 WP_024014342.1 type II secretion system F family protein Machinery gene
  WKQ99_RS05190 (WKQ99_05190) pilB 1103788..1105488 (-) 1701 WP_073475466.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WKQ99_RS05195 (WKQ99_05195) - 1105715..1106152 (+) 438 WP_073475464.1 pilin -
  WKQ99_RS05200 (WKQ99_05200) - 1106451..1106804 (+) 354 WP_016773155.1 BON domain-containing protein -
  WKQ99_RS05205 (WKQ99_05205) - 1107067..1107366 (-) 300 WP_273525934.1 DUF2845 domain-containing protein -
  WKQ99_RS05210 (WKQ99_05210) - 1107363..1110314 (-) 2952 WP_339101038.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62598.62 Da        Isoelectric Point: 6.0450

>NTDB_id=962369 WKQ99_RS05190 WP_073475466.1 1103788..1105488(-) (pilB) [Pseudomonas atacamensis strain EMP42]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLSCLDKETQPRGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=962369 WKQ99_RS05190 WP_073475466.1 1103788..1105488(-) (pilB) [Pseudomonas atacamensis strain EMP42]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAACAAGCGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGCGTGTCGCTGGTCAATTACCTGGTACACAACAAACTGGTCAACAGCCGGCAGATCG
CCGAGATCGCCTCGGAACATTTCGGCATGGCCCTGCTCGATCTCAGCTGCCTCGACAAGGAGACTCAGCCGAGGGGCCTG
GTCAGTGAAAAACTGGTTCGTCAGCATTGCGCCCTGCCCTTGTGGCGGCGCGGCAACAAATTGTTCGTGGGCCTTTCCGA
CCCGAGCAATCAGCAGGCGATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTCGAGGACGACA
AACTCAGCGACGCCATCGACAAGTTCTTCGACAGCCATGCCTCGGGTCTGGAAGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATTGATGACAACAAACAGGACGTGATCGGCGGCCTCGATGCCGACGACGCCCCGGTCGTGCGCTT
CGTGCACAAAATGCTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAAAACTACCGGG
TGCGCATGCGCACCGACGGCATGCTGCGCGAGGTGGCCAAGCCGCCGATTCAGCTGGCCGGGCGTATTGCCGCTCGCCTG
AAGGTGATGGCCAGTCTGGATATTTCCGAACGACGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTTCGCGTCAACACCTTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CACAAATGGGCATCGACGCCTTGGGTTACGAAGCCGAGCAAAAGGCTTTGTACCTGGCCGCGCTGAAACAGCCGCAAGGA
ATGATTCTCGTCACCGGCCCCACCGGCTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAATATCCTCAACACCGTCGA
CATCAATATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCCGCCAAG
GGCTGGACTTTGCCCAGGCGCTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCGGCGGA
AACCCTCACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATTGCCACCTCGGTCAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAATTGTGCGCGCACTGCAAGCGGCCGCTGGAAATCCCCCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAGAACACACCGGACCTGCAACGGTTGATCATGGCCGAAGGCAATTCGCTGGAAATCGACATTCAGA
TGCGCCGCGACGGTTTCGCCGATCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.522

99.823

0.574
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.624

99.117

0.502
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.209

100

0.495
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.837

94.17

0.366