Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WJL51_RS18905 Genome accession   NZ_CP149124
Coordinates   4132723..4133832 (-) Length   369 a.a.
NCBI ID   WP_223950699.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BE2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4127723..4138832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJL51_RS18885 brnQ 4128180..4129487 (-) 1308 WP_011707394.1 branched-chain amino acid transport system II carrier protein -
  WJL51_RS18890 - 4129679..4130389 (-) 711 WP_339330175.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  WJL51_RS18895 srmB 4130518..4131741 (+) 1224 WP_206818813.1 ATP-dependent RNA helicase SrmB -
  WJL51_RS18900 yaaA 4131918..4132691 (-) 774 WP_139705472.1 peroxide stress protein YaaA -
  WJL51_RS18905 pilU 4132723..4133832 (-) 1110 WP_223950699.1 type IVa pilus ATPase TapU Machinery gene
  WJL51_RS18910 pilT 4133861..4134895 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  WJL51_RS18915 - 4134935..4135636 (+) 702 WP_339330176.1 YggS family pyridoxal phosphate-dependent enzyme -
  WJL51_RS18920 proC 4135695..4136519 (+) 825 WP_339330177.1 pyrroline-5-carboxylate reductase -
  WJL51_RS18925 - 4136560..4137111 (+) 552 WP_016351969.1 YggT family protein -
  WJL51_RS18930 yggU 4137111..4137410 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  WJL51_RS18935 - 4137429..4137848 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  WJL51_RS18940 - 4137905..4138216 (-) 312 WP_101149747.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41082.27 Da        Isoelectric Point: 6.5657

>NTDB_id=956668 WJL51_RS18905 WP_223950699.1 4132723..4133832(-) (pilU) [Aeromonas hydrophila strain BE2]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLVEAPLDKARALALVKETLSQEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=956668 WJL51_RS18905 WP_223950699.1 4132723..4133832(-) (pilU) [Aeromonas hydrophila strain BE2]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCACTGGTAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGTAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGTTCGTTGCGCCAGGCCCCTGACGTGATCCTGATCGGCGAAATTCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCATCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTAGCTCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGTCGCTGTGCGGCCTTCGAGGTACTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGAGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36