Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LLI816_RS10645 Genome accession   NZ_CP149095
Coordinates   2091163..2092101 (-) Length   312 a.a.
NCBI ID   WP_031561106.1    Uniprot ID   A0AAP8DEI2
Organism   Lactococcus lactis subsp. lactis strain I8-16     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2086163..2097101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLI816_RS10605 (LLI816_10600) - 2087051..2087893 (-) 843 WP_063283655.1 metal ABC transporter substrate-binding protein -
  LLI816_RS10610 (LLI816_10605) - 2087890..2088327 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  LLI816_RS10615 (LLI816_10610) comGG 2088408..2088692 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLI816_RS10620 (LLI816_10615) comGF 2088731..2089177 (-) 447 WP_031296844.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLI816_RS10625 (LLI816_10620) comGE 2089140..2089436 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLI816_RS10630 (LLI816_10625) comGD 2089408..2089806 (-) 399 WP_021214886.1 competence type IV pilus minor pilin ComGD Machinery gene
  LLI816_RS10635 (LLI816_10630) comGC 2089799..2090182 (-) 384 WP_010906318.1 competence type IV pilus major pilin ComGC Machinery gene
  LLI816_RS10640 (LLI816_10635) comGB 2090196..2091269 (-) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLI816_RS10645 (LLI816_10640) comGA 2091163..2092101 (-) 939 WP_031561106.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35707.88 Da        Isoelectric Point: 5.9473

>NTDB_id=956630 LLI816_RS10645 WP_031561106.1 2091163..2092101(-) (comGA) [Lactococcus lactis subsp. lactis strain I8-16]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=956630 LLI816_RS10645 WP_031561106.1 2091163..2092101(-) (comGA) [Lactococcus lactis subsp. lactis strain I8-16]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATAGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.923

100

0.769
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519