Detailed information NTG0956625

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LLI816_RS10620
• Genome accession: NZ_CP149095
• Coordinates: 2088731..2089177 (-)
• Length: 148 a.a.
• NCBI ID: WP_031296844.1
• Uniprot ID: A0AAC9R3D6
• Organism: Lactococcus lactis subsp. lactis strain I8-16
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2083731..2094177

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLI816_RS10585 (LLI816_10580)	-	2083848..2084225 (-)	378	WP_063283703.1	pyridoxamine 5'-phosphate oxidase family protein	-
LLI816_RS10590 (LLI816_10585)	-	2084430..2085296 (+)	867	WP_010906309.1	RluA family pseudouridine synthase	-
LLI816_RS10595 (LLI816_10590)	-	2085335..2086144 (-)	810	WP_010906310.1	metal ABC transporter permease	-
LLI816_RS10600 (LLI816_10595)	-	2086137..2086874 (-)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
LLI816_RS10605 (LLI816_10600)	-	2087051..2087893 (-)	843	WP_063283655.1	metal ABC transporter substrate-binding protein	-
LLI816_RS10610 (LLI816_10605)	-	2087890..2088327 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
LLI816_RS10615 (LLI816_10610)	comGG	2088408..2088692 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
LLI816_RS10620 (LLI816_10615)	comGF	2088731..2089177 (-)	447	WP_031296844.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLI816_RS10625 (LLI816_10620)	comGE	2089140..2089436 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLI816_RS10630 (LLI816_10625)	comGD	2089408..2089806 (-)	399	WP_021214886.1	competence type IV pilus minor pilin ComGD	Machinery gene
LLI816_RS10635 (LLI816_10630)	comGC	2089799..2090182 (-)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
LLI816_RS10640 (LLI816_10635)	comGB	2090196..2091269 (-)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLI816_RS10645 (LLI816_10640)	comGA	2091163..2092101 (-)	939	WP_031561106.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.85 Da
• Isoelectric Point: 8.6780

>NTDB_id=956625 LLI816_RS10620 WP_031296844.1 2088731..2089177(-) (comGF) [Lactococcus lactis subsp. lactis strain I8-16]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=956625 LLI816_RS10620 WP_031296844.1 2088731..2089177(-) (comGF) [Lactococcus lactis subsp. lactis strain I8-16]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385