Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   AAA985_RS10450 Genome accession   NZ_AP028603
Coordinates   1981145..1982086 (-) Length   313 a.a.
NCBI ID   WP_000249559.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain Pne2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1976145..1987086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAA985_RS10410 (TKY181970_20290) comGH 1976631..1977584 (-) 954 WP_000345126.1 class I SAM-dependent methyltransferase Machinery gene
  AAA985_RS10415 - 1977645..1978239 (-) 595 Protein_2027 methyltransferase domain-containing protein -
  AAA985_RS10420 (TKY181970_20320) comGG/cglG 1978376..1978789 (-) 414 WP_000265620.1 competence type IV pilus minor pilin ComGG Machinery gene
  AAA985_RS10425 (TKY181970_20330) comGF/cglF 1978767..1979228 (-) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  AAA985_RS10430 (TKY181970_20340) comGE/cglE 1979191..1979493 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  AAA985_RS10435 (TKY181970_20350) comGD/cglD 1979456..1979860 (-) 405 WP_000588023.1 competence type IV pilus minor pilin ComGD Machinery gene
  AAA985_RS10440 (TKY181970_20360) comGC/cglC 1979853..1980179 (-) 327 WP_050260822.1 comG operon protein ComGC Machinery gene
  AAA985_RS10445 (TKY181970_20370) comGB/cglB 1980181..1981197 (-) 1017 WP_073177425.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAA985_RS10450 (TKY181970_20380) comGA/cglA/cilD 1981145..1982086 (-) 942 WP_000249559.1 competence type IV pilus ATPase ComGA Machinery gene
  AAA985_RS10455 (TKY181970_20390) - 1982162..1982527 (-) 366 WP_000286415.1 DUF1033 family protein -
  AAA985_RS10460 (TKY181970_20400) - 1982794..1984049 (+) 1256 WP_088804233.1 ISL3 family transposase -
  AAA985_RS10465 (TKY181970_20410) - 1984098..1985156 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  AAA985_RS10470 (TKY181970_20420) nagA 1985319..1986470 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35489.42 Da        Isoelectric Point: 6.1829

>NTDB_id=95297 AAA985_RS10450 WP_000249559.1 1981145..1982086(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain Pne2]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHGLSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=95297 AAA985_RS10450 WP_000249559.1 1981145..1982086(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain Pne2]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGCTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGAAGTGGTAAGACGACCTTGATGCATGGATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGATATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.403

100

0.984
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comGA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comGA Streptococcus mutans UA159

66.238

99.361

0.658
  comGA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment