Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   WHO69_RS12085 Genome accession   NZ_CP148108
Coordinates   2657539..2658762 (+) Length   407 a.a.
NCBI ID   WP_020333949.1    Uniprot ID   -
Organism   Vibrio natriegens isolate FELIX_MS472     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2652539..2663762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO69_RS12065 ampD 2653572..2654117 (-) 546 WP_020333945.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WHO69_RS12070 nadC 2654210..2655097 (+) 888 WP_020333946.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WHO69_RS12075 pilA 2655364..2655822 (+) 459 WP_020333947.1 pilin Machinery gene
  WHO69_RS12080 pilB 2655823..2657508 (+) 1686 WP_020333948.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WHO69_RS12085 pilC 2657539..2658762 (+) 1224 WP_020333949.1 type II secretion system F family protein Machinery gene
  WHO69_RS12090 pilD 2658830..2659699 (+) 870 WP_020333950.1 A24 family peptidase Machinery gene
  WHO69_RS12095 coaE 2659700..2660314 (+) 615 WP_020333951.1 dephospho-CoA kinase -
  WHO69_RS12100 zapD 2660341..2661081 (+) 741 WP_020333952.1 cell division protein ZapD -
  WHO69_RS12105 yacG 2661155..2661349 (+) 195 WP_014232956.1 DNA gyrase inhibitor YacG -
  WHO69_RS12110 rplS 2661674..2662027 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  WHO69_RS12115 trmD 2662068..2662829 (-) 762 WP_014232957.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  WHO69_RS12120 rimM 2662857..2663405 (-) 549 WP_014232958.1 ribosome maturation factor RimM -
  WHO69_RS12125 rpsP 2663436..2663684 (-) 249 WP_005379962.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45051.28 Da        Isoelectric Point: 10.3231

>NTDB_id=950686 WHO69_RS12085 WP_020333949.1 2657539..2658762(+) (pilC) [Vibrio natriegens isolate FELIX_MS472]
MKTTTPQLKNFRWKGINSSGKKTSGQTLAMSEVEVRERLSAQHIKIKKLKKSSISFITKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSNNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASAHFDALYTDLISTGELSGNLAEVFERLATYREK
SEELRAKVIKALIYPAMVMLVALGVSYLMLTQVIPEFEKMFSGFGADLPWFTRQVLDLSAWMQNWGSFIALGALSLIISA
RILVKRSDSFRLMMDRAILRIPVLGAVLSKAAIAKFSRTLATSFSAGIPILTSLKTTSKTSGNLHYQLAVEEVYRDTAAG
MPMYVAMRNCDVFPELVLQMVMIGEESGRLDEMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=950686 WHO69_RS12085 WP_020333949.1 2657539..2658762(+) (pilC) [Vibrio natriegens isolate FELIX_MS472]
ATGAAAACGACGACCCCACAACTAAAGAACTTCCGCTGGAAAGGCATTAATAGCTCAGGAAAAAAAACTTCTGGGCAAAC
GCTGGCCATGAGCGAAGTGGAAGTACGCGAACGACTCAGCGCTCAGCACATAAAAATCAAAAAACTGAAAAAAAGTAGTA
TTTCTTTTATAACCAAACTGAGTCATCGGGTAAAAGGCAAAGACATCACCGTTTTTACTCGTCAAATTTCGACTATGCTG
GTCACTGGTGTCCCACTCGTTCAGGCTTTAAAATTGGTTTCCAATAACCATAAAAAAGCCGAGATGAAGTCTATTTTAAT
GAGCGTAACTCGAGCGGTTGAAGCGGGTACACCGATGTCGAAAGCAATGCGCACCGCCAGCGCACATTTTGACGCACTTT
ATACTGACCTTATCTCAACCGGAGAACTCTCAGGCAATCTGGCAGAAGTCTTCGAACGACTGGCCACTTACCGTGAAAAA
AGTGAGGAACTCAGAGCGAAAGTCATCAAAGCCCTGATCTACCCAGCCATGGTTATGTTGGTTGCGCTCGGCGTGTCCTA
TCTAATGCTCACTCAAGTTATCCCTGAGTTTGAAAAAATGTTTTCTGGGTTTGGTGCAGATTTACCTTGGTTCACCAGAC
AAGTACTAGACTTGTCTGCGTGGATGCAAAATTGGGGTTCATTTATCGCTCTTGGGGCACTTAGCCTAATTATATCAGCA
AGAATACTGGTCAAACGTTCTGATTCTTTTCGCTTGATGATGGATCGAGCCATATTGAGAATTCCCGTTCTTGGCGCTGT
GTTGTCCAAGGCTGCGATTGCTAAATTTAGCCGAACCCTGGCCACCAGCTTTAGCGCGGGTATTCCTATTCTGACCAGTT
TAAAAACCACCTCCAAAACGTCAGGAAACCTGCACTATCAGCTGGCAGTCGAAGAAGTCTATCGTGATACAGCAGCTGGT
ATGCCAATGTATGTCGCAATGCGTAACTGCGATGTTTTCCCTGAATTAGTGCTACAAATGGTGATGATTGGAGAAGAATC
AGGCAGGCTTGATGAGATGCTCAATAAAGTCGCAACTATTTATGAGTTCGAAGTAGATAATACCGTCGATAACCTAAGTA
AGATTCTTGAACCGCTGATTATTGTTTTTCTTGGTGTGGTCGTTGGTGGATTAGTGACAGCAATGTACCTGCCAATCTTT
AACTTAATGAGTGTATTAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.749

100

0.857
  pilC Vibrio cholerae strain A1552

73.333

99.509

0.73
  pilC Acinetobacter baylyi ADP1

42

98.28

0.413
  pilC Legionella pneumophila strain ERS1305867

41.235

99.509

0.41
  pilG Neisseria gonorrhoeae MS11

40.447

99.017

0.4
  pilG Neisseria meningitidis 44/76-A

40.199

99.017

0.398
  pilC Acinetobacter baumannii D1279779

40.5

98.28

0.398
  pilC Pseudomonas stutzeri DSM 10701

40.399

98.526

0.398