Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WHO69_RS12075 Genome accession   NZ_CP148108
Coordinates   2655364..2655822 (+) Length   152 a.a.
NCBI ID   WP_020333947.1    Uniprot ID   A0AAN0Y3X9
Organism   Vibrio natriegens isolate FELIX_MS472     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2650364..2660822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHO69_RS12060 pdhR 2652395..2653162 (-) 768 WP_014232947.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  WHO69_RS12065 ampD 2653572..2654117 (-) 546 WP_020333945.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WHO69_RS12070 nadC 2654210..2655097 (+) 888 WP_020333946.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WHO69_RS12075 pilA 2655364..2655822 (+) 459 WP_020333947.1 pilin Machinery gene
  WHO69_RS12080 pilB 2655823..2657508 (+) 1686 WP_020333948.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WHO69_RS12085 pilC 2657539..2658762 (+) 1224 WP_020333949.1 type II secretion system F family protein Machinery gene
  WHO69_RS12090 pilD 2658830..2659699 (+) 870 WP_020333950.1 A24 family peptidase Machinery gene
  WHO69_RS12095 coaE 2659700..2660314 (+) 615 WP_020333951.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15920.27 Da        Isoelectric Point: 7.7804

>NTDB_id=950684 WHO69_RS12075 WP_020333947.1 2655364..2655822(+) (pilA) [Vibrio natriegens isolate FELIX_MS472]
MKSNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSALGVGLANITALKTNIEDYIATEGSFPSTTSGTKADFT
RLGTVEDMGDGNVLITPTSDGSLDGTVKFTFDAGVVNTQKIQLVRSGGLWICETTAASEISPKGCAAGSTIK

Nucleotide


Download         Length: 459 bp        

>NTDB_id=950684 WHO69_RS12075 WP_020333947.1 2655364..2655822(+) (pilA) [Vibrio natriegens isolate FELIX_MS472]
ATGAAGTCAAATAAACAGAAAAAACAGCAAGGCTTTACGCTGATTGAATTAATGATCGTGGTGGCGGTAATAGGGGTTTT
GGCAGCAATTGCTATCCCTCAGTACCAAAACTACGTAAAGAAATCAGCTTTAGGAGTAGGCCTAGCAAATATTACTGCTC
TGAAAACTAACATCGAGGATTATATTGCTACTGAAGGTAGCTTTCCTTCAACAACATCAGGTACCAAAGCTGATTTTACG
CGGCTCGGTACCGTAGAAGACATGGGAGATGGCAACGTTCTAATCACCCCTACAAGCGACGGCTCTTTAGATGGAACAGT
AAAATTCACCTTTGATGCAGGTGTCGTCAATACACAAAAGATTCAGTTAGTTCGATCTGGTGGACTTTGGATATGTGAAA
CGACAGCAGCAAGTGAAATATCTCCAAAAGGTTGTGCTGCTGGTTCAACGATTAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

77.632

100

0.776
  pilA Vibrio cholerae C6706

77.632

100

0.776
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

77.632

100

0.776
  pilA Pseudomonas aeruginosa PAK

43.226

100

0.441
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

97.368

0.414
  pilA Acinetobacter baumannii strain A118

40.397

99.342

0.401
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.86

94.079

0.375