Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WGK56_RS03785 Genome accession   NZ_CP148001
Coordinates   738110..739504 (+) Length   464 a.a.
NCBI ID   WP_015701560.1    Uniprot ID   -
Organism   Haemophilus influenzae strain AR2632     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 733110..744504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WGK56_RS03760 (WGK56_03750) rsmE 733126..733863 (-) 738 WP_015701564.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WGK56_RS03765 (WGK56_03755) lnt 733913..735442 (-) 1530 WP_015701563.1 apolipoprotein N-acyltransferase -
  WGK56_RS03770 (WGK56_03760) corC 735465..736364 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  WGK56_RS03775 (WGK56_03765) ampD 736996..737550 (-) 555 WP_041175038.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WGK56_RS03780 (WGK56_03770) pilA 737664..738113 (+) 450 WP_015701561.1 prepilin peptidase-dependent pilin Machinery gene
  WGK56_RS03785 (WGK56_03775) pilB 738110..739504 (+) 1395 WP_015701560.1 GspE/PulE family protein Machinery gene
  WGK56_RS03790 (WGK56_03780) pilC 739501..740718 (+) 1218 WP_015701559.1 type II secretion system F family protein Machinery gene
  WGK56_RS03795 (WGK56_03785) pilD 740715..741407 (+) 693 WP_041175037.1 prepilin peptidase Machinery gene
  WGK56_RS03800 (WGK56_03790) rho 741463..742725 (-) 1263 WP_015701557.1 transcription termination factor Rho -
  WGK56_RS03805 (WGK56_03795) metJ 742973..743290 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  WGK56_RS03810 (WGK56_03800) cueR 743304..743690 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  WGK56_RS03815 (WGK56_03805) - 743767..743973 (+) 207 WP_005686717.1 heavy-metal-associated domain-containing protein -
  WGK56_RS03820 (WGK56_03810) - 744059..744265 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52909.44 Da        Isoelectric Point: 5.6415

>NTDB_id=949948 WGK56_RS03785 WP_015701560.1 738110..739504(+) (pilB) [Haemophilus influenzae strain AR2632]
MTSYALLHTQRVTAKNGEVFTISPDLWERNRQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELGGIIQSQINPQIGLDFSRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKTL

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=949948 WGK56_RS03785 WP_015701560.1 738110..739504(+) (pilB) [Haemophilus influenzae strain AR2632]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCGGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGTGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAGCCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACACGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGGTGGCATTATTCAAAGCCAAATTAACCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
ACTTTCTTACGCCAAGATCCCGATATTATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTGCGTGC
TGCCCAAACTGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGCTTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAATAGCTTATTGCTCGTTATTGCTCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAACTTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

97.408

99.784

0.972
  pilB Haemophilus influenzae Rd KW20

96.983

100

0.97
  pilB Glaesserella parasuis strain SC1401

58.333

98.276

0.573
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

38.763

100

0.405
  pilF Neisseria gonorrhoeae MS11

36.765

100

0.377
  pilF Thermus thermophilus HB27

35.95

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.179

100

0.375