Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WGK56_RS03780 Genome accession   NZ_CP148001
Coordinates   737664..738113 (+) Length   149 a.a.
NCBI ID   WP_015701561.1    Uniprot ID   -
Organism   Haemophilus influenzae strain AR2632     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 732664..743113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WGK56_RS03760 (WGK56_03750) rsmE 733126..733863 (-) 738 WP_015701564.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WGK56_RS03765 (WGK56_03755) lnt 733913..735442 (-) 1530 WP_015701563.1 apolipoprotein N-acyltransferase -
  WGK56_RS03770 (WGK56_03760) corC 735465..736364 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  WGK56_RS03775 (WGK56_03765) ampD 736996..737550 (-) 555 WP_041175038.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WGK56_RS03780 (WGK56_03770) pilA 737664..738113 (+) 450 WP_015701561.1 prepilin peptidase-dependent pilin Machinery gene
  WGK56_RS03785 (WGK56_03775) pilB 738110..739504 (+) 1395 WP_015701560.1 GspE/PulE family protein Machinery gene
  WGK56_RS03790 (WGK56_03780) pilC 739501..740718 (+) 1218 WP_015701559.1 type II secretion system F family protein Machinery gene
  WGK56_RS03795 (WGK56_03785) pilD 740715..741407 (+) 693 WP_041175037.1 prepilin peptidase Machinery gene
  WGK56_RS03800 (WGK56_03790) rho 741463..742725 (-) 1263 WP_015701557.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15492.75 Da        Isoelectric Point: 8.0914

>NTDB_id=949947 WGK56_RS03780 WP_015701561.1 737664..738113(+) (pilA) [Haemophilus influenzae strain AR2632]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTSCTGGKNGIA
ADITTAKGYVKSVTTSNGAITVAGNGTLDGMSYTLTAEGDSAKGVTWKTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=949947 WGK56_RS03780 WP_015701561.1 737664..738113(+) (pilA) [Haemophilus influenzae strain AR2632]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGTTTTACATTAATCGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCGTCTGCGC
CTTATAAGGCTGATGTAGAATTATGTGTATATAGCACAAATGAAACAACAAGCTGTACAGGTGGAAAAAATGGTATTGCA
GCGGATATTACAACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAGCGGGTAATGGTAC
ATTAGATGGAATGAGTTACACGCTCACGGCTGAAGGCGATAGTGCAAAAGGCGTTACATGGAAAACCACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

91.275

100

0.913
  pilA Haemophilus influenzae Rd KW20

87.248

100

0.872
  pilA Glaesserella parasuis strain SC1401

57.047

100

0.57
  pilA2 Legionella pneumophila strain ERS1305867

45.038

87.919

0.396
  pilA2 Legionella pneumophila str. Paris

44.615

87.248

0.389
  pilA Vibrio campbellii strain DS40M4

38.298

94.631

0.362