Detailed information NTG0942555

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V7R83_RS04115
• Genome accession: NZ_CP146288
• Coordinates: 1005713..1006846 (+)
• Length: 377 a.a.
• NCBI ID: WP_040530489.1
• Uniprot ID: -
• Organism: Lautropia mirabilis ATCC 51599
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1000713..1011846

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V7R83_RS04095 (V7R83_04095)	-	1000854..1001867 (-)	1014	WP_005672167.1	metal ABC transporter substrate-binding protein	-
V7R83_RS04100 (V7R83_04100)	-	1002248..1002961 (-)	714	WP_005672168.1	autoinducer binding domain-containing protein	-
V7R83_RS04105 (V7R83_04105)	-	1003238..1003948 (-)	711	WP_005672170.1	autoinducer binding domain-containing protein	-
V7R83_RS04110 (V7R83_04110)	pilT	1004549..1005592 (+)	1044	WP_040529606.1	type IV pilus twitching motility protein PilT	Machinery gene
V7R83_RS04115 (V7R83_04115)	pilU	1005713..1006846 (+)	1134	WP_040530489.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V7R83_RS04120 (V7R83_04120)	-	1006965..1007780 (+)	816	WP_005672178.1	carbonic anhydrase family protein	-
V7R83_RS04125 (V7R83_04125)	-	1008077..1008919 (+)	843	WP_040530491.1	carbonic anhydrase family protein	-
V7R83_RS04130 (V7R83_04130)	-	1009043..1010407 (+)	1365	WP_232002019.1	glycerophosphodiester phosphodiesterase	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41882.32 Da
• Isoelectric Point: 6.9783

>NTDB_id=942555 V7R83_RS04115 WP_040530489.1 1005713..1006846(+) (pilU) [Lautropia mirabilis ATCC 51599]
MEREQASAFLYDLLRLMIAKDGSDLFLTVDFPPAFKISGKMVPVSDKPLTTQHTIELARALMNDRQAAEFEANKELNFAI
SPGGIGRFRVNAFTQMGRTGLVLRVIKSEIPDLATLNLPPVMSELSMTQRGLILMVGATGSGKSTTLAAMIGHRNEHSHG
HIITIEDPVEFVHVHKNCIVTHREVGVDTLDWHVALKNTLRQAPDVIMLGEIRDRETMEHAVAFAETGHLCMGTLHANSA
NQALDRIINFFPDERRQQLLMDLSLNLKAVISQRLVPAEGRKGRVAAVEIMINSPLISDLIFKGSVSEIKEIMKKSRELG
MQTFDQHLFDLFEAGQISYENALRNADSVNDLRLNIKLNSKRRSEDPTAASSKLGIV

Nucleotide

• Length: 1134 bp

>NTDB_id=942555 V7R83_RS04115 WP_040530489.1 1005713..1006846(+) (pilU) [Lautropia mirabilis ATCC 51599]
ATGGAACGTGAGCAGGCGTCGGCCTTCCTGTACGACCTGCTGCGGCTGATGATCGCCAAGGACGGTTCGGACCTGTTCCT
GACCGTGGACTTCCCGCCGGCCTTCAAGATCAGCGGCAAGATGGTGCCGGTCTCGGACAAGCCGCTGACCACCCAGCACA
CCATCGAGCTGGCGCGGGCGCTGATGAACGACCGCCAGGCGGCCGAGTTCGAGGCCAACAAGGAACTGAACTTCGCCATC
AGCCCGGGCGGCATCGGCCGCTTCCGGGTCAATGCCTTCACGCAGATGGGGCGCACGGGCCTGGTGCTGCGGGTGATCAA
GAGCGAGATTCCCGATCTGGCCACCCTGAACCTGCCGCCGGTGATGAGCGAGCTGTCGATGACGCAGCGCGGCCTGATCC
TGATGGTGGGCGCCACCGGCTCGGGCAAGTCCACCACGCTGGCCGCGATGATCGGCCACCGCAACGAGCACAGCCACGGC
CACATCATCACCATCGAGGACCCGGTGGAATTCGTGCACGTGCACAAGAACTGCATCGTCACACACCGCGAGGTGGGTGT
CGACACGCTGGACTGGCACGTCGCACTGAAGAACACGCTGCGTCAGGCGCCCGACGTGATCATGCTGGGCGAGATCCGTG
ACCGCGAGACGATGGAACATGCGGTGGCCTTTGCCGAGACCGGCCACCTGTGCATGGGCACGCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGCATCATCAACTTCTTCCCCGACGAGCGCCGCCAGCAGCTGCTGATGGATCTGTCGCTGAACCT
GAAGGCGGTGATCTCGCAGCGCCTGGTGCCGGCCGAAGGGCGCAAGGGGCGCGTGGCTGCGGTGGAGATCATGATCAACT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCAGCGTCAGCGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTGGGC
ATGCAGACCTTCGACCAGCACCTCTTCGACCTGTTCGAGGCCGGGCAGATCAGCTACGAGAACGCACTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGAACATCAAGCTCAACAGCAAGCGCCGGAGCGAGGATCCCACCGCGGCCAGCAGCAAGC
TGGGCATCGTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (87-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.773	96.021	0.584
pilU	Acinetobacter baylyi ADP1	55.84	93.103	0.52
pilU	Vibrio cholerae strain A1552	52.113	94.164	0.491
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.104	89.39	0.403
pilT	Pseudomonas aeruginosa PAK	42.73	89.39	0.382
pilT	Pseudomonas stutzeri DSM 10701	41.543	89.39	0.371
pilT	Legionella pneumophila strain Lp02	41.742	88.329	0.369
pilT	Legionella pneumophila strain ERS1305867	41.742	88.329	0.369
pilT	Acinetobacter baylyi ADP1	41.441	88.329	0.366