Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V6090_RS17800 Genome accession   NZ_CP145798
Coordinates   3924662..3925771 (-) Length   369 a.a.
NCBI ID   WP_406596060.1    Uniprot ID   -
Organism   Aeromonas veronii strain CARB25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3919662..3930771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6090_RS17770 (V6090_17775) - 3920113..3921120 (-) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -
  V6090_RS17775 (V6090_17780) glnK 3921379..3921717 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  V6090_RS17780 (V6090_17785) arfB 3921919..3922335 (+) 417 WP_279489791.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  V6090_RS17785 (V6090_17790) - 3922368..3922859 (+) 492 WP_279489792.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  V6090_RS17790 (V6090_17795) - 3923221..3923646 (-) 426 WP_005340360.1 hypothetical protein -
  V6090_RS17795 (V6090_17800) yaaA 3923841..3924614 (-) 774 WP_040067414.1 peroxide stress protein YaaA -
  V6090_RS17800 (V6090_17805) pilU 3924662..3925771 (-) 1110 WP_406596060.1 type IVa pilus ATPase TapU Machinery gene
  V6090_RS17805 (V6090_17810) pilT 3925800..3926834 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  V6090_RS17810 (V6090_17815) - 3926874..3927575 (+) 702 WP_019445603.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6090_RS17815 (V6090_17820) proC 3927717..3928541 (+) 825 WP_279489793.1 pyrroline-5-carboxylate reductase -
  V6090_RS17820 (V6090_17825) - 3928553..3929104 (+) 552 WP_005340343.1 YggT family protein -
  V6090_RS17825 (V6090_17830) yggU 3929104..3929406 (+) 303 WP_005340341.1 DUF167 family protein YggU -
  V6090_RS17830 (V6090_17835) - 3929450..3929869 (+) 420 WP_279489794.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41106.20 Da        Isoelectric Point: 6.5043

>NTDB_id=939894 V6090_RS17800 WP_406596060.1 3924662..3925771(-) (pilU) [Aeromonas veronii strain CARB25]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALMLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=939894 V6090_RS17800 WP_406596060.1 3924662..3925771(-) (pilU) [Aeromonas veronii strain CARB25]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCAGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCGCTGATGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCTGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAAATTCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTTGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCATTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGGAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGACAAGCACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCCGCGTTCGAGATCCTGCTCAATACTCCGCTCATCACTGACATTA
TTCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGTACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36