Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   NGK70_RS05825 Genome accession   NZ_AP025730
Coordinates   1320023..1320499 (+) Length   158 a.a.
NCBI ID   WP_251972334.1    Uniprot ID   -
Organism   Sphaerotilus microaerophilus strain FB-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1315023..1325499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGK70_RS05805 (CATMQ487_11580) lptE 1315824..1316396 (+) 573 WP_251973691.1 LPS assembly lipoprotein LptE -
  NGK70_RS05810 (CATMQ487_11590) holA 1316419..1317510 (+) 1092 WP_251972331.1 DNA polymerase III subunit delta -
  NGK70_RS05815 (CATMQ487_11600) groES 1317734..1318024 (+) 291 WP_251972332.1 co-chaperone GroES -
  NGK70_RS05820 (CATMQ487_11610) groL 1318089..1319732 (+) 1644 WP_251972333.1 chaperonin GroEL -
  NGK70_RS05825 (CATMQ487_11620) pilE 1320023..1320499 (+) 477 WP_251972334.1 pilin Machinery gene
  NGK70_RS05830 (CATMQ487_11640) - 1320605..1322515 (+) 1911 WP_251972335.1 O-antigen ligase family protein -
  NGK70_RS05835 (CATMQ487_11650) moaC 1322526..1323056 (-) 531 WP_251972336.1 cyclic pyranopterin monophosphate synthase MoaC -
  NGK70_RS05840 (CATMQ487_11660) - 1323239..1324864 (+) 1626 WP_251972337.1 M48 family metalloprotease -
  NGK70_RS05845 (CATMQ487_11670) - 1324895..1325251 (-) 357 WP_251972338.1 phage holin family protein -

Sequence

Protein


Download         Length: 158 a.a.        Molecular weight: 16409.96 Da        Isoelectric Point: 8.4716

>NTDB_id=93797 NGK70_RS05825 WP_251972334.1 1320023..1320499(+) (pilE) [Sphaerotilus microaerophilus strain FB-5]
MKRVQQGFTLIELMIVVAIIGILAAVALPAYQDYTKRAKMSEVILAASQCRTSISEAIQSMPTTSIGTANGWGCESTSPS
KYVASVATGINGVVTVTTQGFNDAAIDGKTITLTPYSDIAMANAIDVSAAAKDQGKQVAAWKCAAGTLTPKYLPGSCK

Nucleotide


Download         Length: 477 bp        

>NTDB_id=93797 NGK70_RS05825 WP_251972334.1 1320023..1320499(+) (pilE) [Sphaerotilus microaerophilus strain FB-5]
ATGAAACGTGTTCAACAGGGCTTCACGCTGATCGAACTGATGATCGTCGTGGCGATCATCGGCATCTTGGCTGCCGTGGC
GCTGCCGGCTTATCAGGATTACACCAAGCGCGCGAAGATGTCTGAAGTCATCCTGGCCGCGTCGCAGTGCCGCACGTCGA
TCAGCGAGGCTATCCAGTCGATGCCGACGACGTCGATCGGTACGGCGAATGGATGGGGCTGTGAATCCACTAGTCCCTCG
AAGTACGTTGCGTCGGTGGCCACTGGTATCAACGGTGTTGTCACGGTGACCACCCAGGGTTTCAATGACGCAGCCATCGA
TGGTAAGACCATCACGCTGACGCCTTATTCGGACATTGCAATGGCTAATGCCATCGACGTGTCGGCCGCCGCCAAGGATC
AGGGCAAGCAAGTCGCCGCGTGGAAGTGCGCGGCGGGCACGCTCACCCCCAAATATCTCCCGGGTTCCTGCAAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(37-158)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.899

100

0.5
  pilA Ralstonia pseudosolanacearum GMI1000

42.197

100

0.462
  pilA2 Legionella pneumophila str. Paris

45.161

98.101

0.443
  pilA2 Legionella pneumophila strain ERS1305867

44.516

98.101

0.437
  comP Acinetobacter baylyi ADP1

39.521

100

0.418
  pilE Neisseria gonorrhoeae MS11

37.931

100

0.418
  pilA/pilA1 Eikenella corrodens VA1

36.628

100

0.399
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.103

98.734

0.386
  pilA/pilAII Pseudomonas stutzeri DSM 10701

35.366

100

0.367

Multiple sequence alignment