Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V0242_RS03520 Genome accession   NZ_CP143514
Coordinates   726551..727660 (-) Length   369 a.a.
NCBI ID   WP_065475661.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain MAH-4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721551..732660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V0242_RS03500 (V0242_03500) brnQ 722008..723315 (-) 1308 WP_106553719.1 branched-chain amino acid transport system II carrier protein -
  V0242_RS03505 (V0242_03505) - 723507..724217 (-) 711 WP_330543364.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  V0242_RS03510 (V0242_03510) srmB 724346..725569 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  V0242_RS03515 (V0242_03515) yaaA 725746..726519 (-) 774 WP_330543367.1 peroxide stress protein YaaA -
  V0242_RS03520 (V0242_03520) pilU 726551..727660 (-) 1110 WP_065475661.1 type IVa pilus ATPase TapU Machinery gene
  V0242_RS03525 (V0242_03525) pilT 727689..728723 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  V0242_RS03530 (V0242_03530) - 728763..729464 (+) 702 WP_330543372.1 YggS family pyridoxal phosphate-dependent enzyme -
  V0242_RS03535 (V0242_03535) proC 729523..730347 (+) 825 WP_330543373.1 pyrroline-5-carboxylate reductase -
  V0242_RS03540 (V0242_03540) - 730388..730939 (+) 552 WP_016351969.1 YggT family protein -
  V0242_RS03545 (V0242_03545) yggU 730939..731238 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  V0242_RS03550 (V0242_03550) - 731257..731676 (+) 420 WP_219314060.1 DUF4426 domain-containing protein -
  V0242_RS03555 (V0242_03555) - 731731..732042 (-) 312 WP_330543375.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41049.19 Da        Isoelectric Point: 6.5035

>NTDB_id=929263 V0242_RS03520 WP_065475661.1 726551..727660(-) (pilU) [Aeromonas hydrophila strain MAH-4]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=929263 V0242_RS03520 WP_065475661.1 726551..727660(-) (pilU) [Aeromonas hydrophila strain MAH-4]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCACACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGTTAGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAAAGCTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGTGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGGCGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363