Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   VW858_RS13740 Genome accession   NZ_CP142811
Coordinates   2800138..2800596 (-) Length   152 a.a.
NCBI ID   WP_002365911.1    Uniprot ID   Q8VT46
Organism   Enterococcus faecalis strain JF1A-4353     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2743778..2807527 2800138..2800596 within 0


Gene organization within MGE regions


Location: 2743778..2807527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VW858_RS13460 (VW858_13460) - 2744481..2745614 (-) 1134 WP_002377959.1 NAD(P)/FAD-dependent oxidoreductase -
  VW858_RS13465 (VW858_13465) - 2745717..2746787 (-) 1071 WP_010710139.1 ammonium transporter -
  VW858_RS13470 (VW858_13470) - 2746981..2747384 (-) 404 Protein_2637 NusG domain II-containing protein -
  VW858_RS13475 (VW858_13475) - 2747436..2747813 (-) 378 WP_002382844.1 hypothetical protein -
  VW858_RS13480 (VW858_13480) rpmF 2747835..2748008 (-) 174 WP_002358539.1 50S ribosomal protein L32 -
  VW858_RS13485 (VW858_13485) - 2748347..2749402 (+) 1056 WP_002401430.1 YibE/F family protein -
  VW858_RS13490 (VW858_13490) - 2749399..2750163 (+) 765 WP_002358536.1 YibE/F family protein -
  VW858_RS13495 (VW858_13495) - 2750796..2751278 (-) 483 WP_002358535.1 PTS glucose transporter subunit IIA -
  VW858_RS13500 (VW858_13500) - 2751289..2752791 (-) 1503 WP_002358534.1 PTS transporter subunit EIIC -
  VW858_RS13505 (VW858_13505) - 2752784..2753491 (-) 708 WP_002358533.1 N-acetylmannosamine-6-phosphate 2-epimerase -
  VW858_RS13510 (VW858_13510) - 2753647..2754465 (+) 819 WP_002358531.1 MurR/RpiR family transcriptional regulator -
  VW858_RS13515 (VW858_13515) - 2754921..2755103 (-) 183 WP_002358529.1 hypothetical protein -
  VW858_RS13520 (VW858_13520) - 2755212..2755831 (-) 620 Protein_2647 recombinase family protein -
  VW858_RS13525 (VW858_13525) - 2756015..2756635 (+) 621 WP_033591050.1 recombinase family protein -
  VW858_RS13530 (VW858_13530) - 2756681..2757418 (-) 738 WP_002401428.1 hypothetical protein -
  VW858_RS13535 (VW858_13535) - 2757406..2757507 (+) 102 Protein_2650 IS30 family transposase -
  VW858_RS13540 (VW858_13540) - 2757643..2758059 (-) 417 WP_002358522.1 hypothetical protein -
  VW858_RS13545 (VW858_13545) - 2758189..2759397 (-) 1209 WP_002358521.1 YSIRK-targeted surface antigen transcriptional regulator -
  VW858_RS13550 (VW858_13550) - 2759597..2765464 (-) 5868 WP_202580324.1 Rib/alpha-like domain-containing protein -
  VW858_RS13555 (VW858_13555) - 2765739..2766275 (-) 537 WP_002358518.1 Asp23/Gls24 family envelope stress response protein -
  VW858_RS13560 (VW858_13560) - 2766304..2766495 (-) 192 WP_002358517.1 DUF2273 domain-containing protein -
  VW858_RS13565 (VW858_13565) amaP 2766512..2767078 (-) 567 WP_010706722.1 alkaline shock response membrane anchor protein AmaP -
  VW858_RS13570 (VW858_13570) - 2767348..2767635 (-) 288 WP_306418522.1 S41 family peptidase -
  VW858_RS13575 (VW858_13575) - 2767649..2768230 (-) 582 WP_002401490.1 hypothetical protein -
  VW858_RS13580 (VW858_13580) - 2768308..2768775 (-) 468 WP_002370921.1 hypothetical protein -
  VW858_RS13585 (VW858_13585) - 2769212..2769775 (-) 564 WP_002370925.1 hypothetical protein -
  VW858_RS13590 (VW858_13590) - 2770128..2771111 (-) 984 WP_002368282.1 site-2 protease family protein -
  VW858_RS13595 (VW858_13595) - 2771112..2772350 (-) 1239 WP_002368283.1 S8 family serine peptidase -
  VW858_RS13600 (VW858_13600) - 2772347..2774491 (-) 2145 WP_002370927.1 peptidase domain-containing ABC transporter -
  VW858_RS13605 (VW858_13605) - 2774503..2777484 (-) 2982 WP_002370929.1 type 2 lanthipeptide synthetase LanM family protein -
  VW858_RS13610 (VW858_13610) cylL-S 2777545..2777736 (-) 192 WP_002358181.1 lanthipeptide cytolysin subunit CylL-S -
  VW858_RS13615 (VW858_13615) cylL-L 2777770..2777976 (-) 207 WP_002358485.1 lanthipeptide cytolysin subunit CylL-L -
  VW858_RS13620 (VW858_13620) cylR2 2778380..2778580 (+) 201 WP_002370931.1 cytolysin regulator CylR2 -
  VW858_RS13625 (VW858_13625) - 2778918..2779337 (-) 420 WP_002370932.1 hypothetical protein -
  VW858_RS13630 (VW858_13630) - 2779405..2780049 (-) 645 WP_002377952.1 IS6 family transposase -
  VW858_RS13635 (VW858_13635) bsh 2780273..2781247 (+) 975 WP_002355428.1 choloylglycine hydrolase -
  VW858_RS13640 (VW858_13640) - 2781916..2782524 (-) 609 Protein_2671 IS6 family transposase -
  VW858_RS13645 (VW858_13645) - 2782556..2782741 (-) 186 WP_002358660.1 hypothetical protein -
  VW858_RS13650 (VW858_13650) - 2782831..2783100 (+) 270 Protein_2673 LPXTG cell wall anchor domain-containing protein -
  VW858_RS13655 (VW858_13655) - 2783228..2784166 (+) 939 WP_002370935.1 2-dehydropantoate 2-reductase -
  VW858_RS13660 (VW858_13660) - 2784192..2785229 (+) 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
  VW858_RS13665 (VW858_13665) - 2785534..2786419 (-) 886 Protein_2676 IS256 family transposase -
  VW858_RS13670 (VW858_13670) - 2786549..2787193 (+) 645 WP_002289351.1 IS6 family transposase -
  VW858_RS13675 (VW858_13675) - 2787384..2787659 (-) 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin -
  VW858_RS13680 (VW858_13680) - 2787652..2787918 (-) 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
  VW858_RS13685 (VW858_13685) - 2788029..2788613 (-) 585 WP_218399387.1 thermonuclease family protein -
  VW858_RS13690 (VW858_13690) - 2788637..2789053 (-) 417 WP_010710134.1 single-stranded DNA-binding protein -
  VW858_RS13695 (VW858_13695) - 2789129..2789377 (-) 249 WP_002360773.1 DUF3850 domain-containing protein -
  VW858_RS13700 (VW858_13700) - 2789390..2789890 (-) 501 WP_002360775.1 DnaJ domain-containing protein -
  VW858_RS13705 (VW858_13705) - 2789923..2790189 (-) 267 WP_002377947.1 hypothetical protein -
  VW858_RS13710 (VW858_13710) - 2790781..2791269 (-) 489 WP_010710133.1 hypothetical protein -
  VW858_RS13715 (VW858_13715) - 2791275..2792060 (-) 786 WP_002360778.1 replication-relaxation family protein -
  VW858_RS13720 (VW858_13720) - 2792583..2794841 (-) 2259 WP_010710132.1 type IV secretory system conjugative DNA transfer family protein -
  VW858_RS13725 (VW858_13725) - 2794828..2797173 (-) 2346 WP_002377940.1 CD3337/EF1877 family mobilome membrane protein -
  VW858_RS13730 (VW858_13730) - 2797186..2797584 (-) 399 WP_002370287.1 lipocalin-like domain-containing protein -
  VW858_RS13735 (VW858_13735) - 2797541..2800033 (-) 2493 WP_002377939.1 ATP-binding protein -
  VW858_RS13740 (VW858_13740) ssb 2800138..2800596 (-) 459 WP_002365911.1 single-stranded DNA-binding protein Machinery gene
  VW858_RS13745 (VW858_13745) - 2800691..2801173 (-) 483 WP_002377938.1 hypothetical protein -
  VW858_RS13750 (VW858_13750) - 2801205..2801594 (-) 390 WP_002360791.1 TcpE family conjugal transfer membrane protein -
  VW858_RS13755 (VW858_13755) - 2801594..2801854 (-) 261 WP_010706916.1 hypothetical protein -
  VW858_RS13760 (VW858_13760) - 2801859..2802893 (-) 1035 WP_002403100.1 conjugal transfer protein -
  VW858_RS13765 (VW858_13765) - 2802886..2803200 (-) 315 WP_002360796.1 hypothetical protein -
  VW858_RS13770 (VW858_13770) - 2803200..2803616 (-) 417 WP_002401419.1 thioredoxin domain-containing protein -
  VW858_RS13775 (VW858_13775) - 2803600..2804217 (-) 618 WP_002377936.1 hypothetical protein -
  VW858_RS13780 (VW858_13780) - 2804220..2805490 (-) 1271 Protein_2699 CHAP domain-containing protein -
  VW858_RS13785 (VW858_13785) - 2805516..2806376 (-) 861 WP_010710128.1 LPXTG cell wall anchor domain-containing protein -
  VW858_RS13790 (VW858_13790) - 2806396..2807274 (-) 879 WP_002377931.1 hypothetical protein -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 17179.08 Da        Isoelectric Point: 4.6511

>NTDB_id=926980 VW858_RS13740 WP_002365911.1 2800138..2800596(-) (ssb) [Enterococcus faecalis strain JF1A-4353]
MINNVTLVGRLTKDPDLRYTQSGTAVGQFTLAINRNFTNANNEREADFINCVIWRKAAESLANYATKGTLIGLTGRIQTR
NYENQQGQRIYVTEVVTESFQLLESREVNEQRKEQATGKATFDKQSMDKPDPLDPFSPENSIVDISDNDLPF

Nucleotide


Download         Length: 459 bp        

>NTDB_id=926980 VW858_RS13740 WP_002365911.1 2800138..2800596(-) (ssb) [Enterococcus faecalis strain JF1A-4353]
TTGATTAATAACGTTACATTAGTTGGACGATTAACCAAAGACCCAGATTTAAGGTATACGCAAAGTGGAACAGCCGTAGG
TCAATTTACGTTGGCCATTAATCGCAACTTTACCAATGCTAACAATGAAAGGGAAGCAGATTTTATCAACTGTGTTATTT
GGCGGAAAGCTGCAGAGTCATTAGCAAATTATGCAACAAAAGGGACTCTGATCGGTTTAACTGGTCGCATTCAAACAAGA
AACTATGAGAATCAACAAGGCCAGCGTATTTATGTAACTGAGGTTGTCACAGAAAGCTTCCAACTATTAGAATCAAGAGA
AGTAAACGAGCAACGAAAAGAACAGGCTACAGGTAAAGCTACGTTTGATAAACAGTCAATGGATAAACCTGATCCTCTGG
ATCCATTTTCGCCAGAAAATAGCATAGTGGATATTTCTGATAATGACCTGCCGTTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VT46

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.471

100

0.632
  ssbA Bacillus subtilis subsp. subtilis str. 168

47.283

100

0.572
  ssbB Bacillus subtilis subsp. subtilis str. 168

56.604

69.737

0.395
  ssbB/cilA Streptococcus mitis NCTC 12261

50

76.316

0.382
  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.774

69.737

0.375
  ssbB/cilA Streptococcus pneumoniae TIGR4

49.138

76.316

0.375
  ssbB/cilA Streptococcus pneumoniae Rx1

48.276

76.316

0.368
  ssbB/cilA Streptococcus pneumoniae D39

48.276

76.316

0.368
  ssbB/cilA Streptococcus pneumoniae R6

48.276

76.316

0.368
  ssbB/cilA Streptococcus mitis SK321

48.276

76.316

0.368