Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   VW859_RS01945 Genome accession   NZ_CP142809
Coordinates   379344..379802 (+) Length   152 a.a.
NCBI ID   WP_002365911.1    Uniprot ID   Q8VT46
Organism   Enterococcus faecalis strain KS2H-124     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 370932..430072 379344..379802 within 0


Gene organization within MGE regions


Location: 370932..430072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VW859_RS01880 (VW859_01880) prgU 371485..371841 (+) 357 WP_010714280.1 pheromone response system RNA-binding regulator PrgU -
  VW859_RS01885 (VW859_01885) - 371863..372249 (+) 387 WP_002377929.1 hypothetical protein -
  VW859_RS01890 (VW859_01890) - 372395..372655 (+) 261 WP_002370667.1 hypothetical protein -
  VW859_RS01895 (VW859_01895) - 372666..373544 (+) 879 WP_002377931.1 hypothetical protein -
  VW859_RS01900 (VW859_01900) - 373564..374424 (+) 861 WP_010710128.1 LPXTG cell wall anchor domain-containing protein -
  VW859_RS01905 (VW859_01905) - 374450..375720 (+) 1271 Protein_351 CHAP domain-containing protein -
  VW859_RS01910 (VW859_01910) - 375723..376340 (+) 618 WP_002377936.1 hypothetical protein -
  VW859_RS01915 (VW859_01915) - 376324..376740 (+) 417 WP_002401419.1 thioredoxin domain-containing protein -
  VW859_RS01920 (VW859_01920) - 376740..377054 (+) 315 WP_002360796.1 hypothetical protein -
  VW859_RS01925 (VW859_01925) - 377047..378081 (+) 1035 WP_002403100.1 conjugal transfer protein -
  VW859_RS01930 (VW859_01930) - 378071..378346 (+) 276 WP_002360793.1 hypothetical protein -
  VW859_RS01935 (VW859_01935) - 378346..378735 (+) 390 WP_002360791.1 TcpE family conjugal transfer membrane protein -
  VW859_RS01940 (VW859_01940) - 378767..379249 (+) 483 WP_002377938.1 hypothetical protein -
  VW859_RS01945 (VW859_01945) ssb 379344..379802 (+) 459 WP_002365911.1 single-stranded DNA-binding protein Machinery gene
  VW859_RS01950 (VW859_01950) - 379907..382399 (+) 2493 WP_002377939.1 ATP-binding protein -
  VW859_RS01955 (VW859_01955) - 382356..382754 (+) 399 WP_002370287.1 lipocalin-like domain-containing protein -
  VW859_RS01960 (VW859_01960) - 382767..385107 (+) 2341 Protein_362 CD3337/EF1877 family mobilome membrane protein -
  VW859_RS01965 (VW859_01965) - 385094..387352 (+) 2259 WP_010710132.1 type IV secretory system conjugative DNA transfer family protein -
  VW859_RS01970 (VW859_01970) - 387875..388660 (+) 786 WP_002360778.1 replication-relaxation family protein -
  VW859_RS01975 (VW859_01975) - 388666..389154 (+) 489 WP_010710133.1 hypothetical protein -
  VW859_RS01980 (VW859_01980) - 389746..390012 (+) 267 WP_002377947.1 hypothetical protein -
  VW859_RS01985 (VW859_01985) - 390045..390545 (+) 501 WP_002360775.1 DnaJ domain-containing protein -
  VW859_RS01990 (VW859_01990) - 390558..390806 (+) 249 WP_002360773.1 DUF3850 domain-containing protein -
  VW859_RS01995 (VW859_01995) - 390882..391298 (+) 417 WP_010710134.1 single-stranded DNA-binding protein -
  VW859_RS02000 (VW859_02000) - 391322..391906 (+) 585 WP_002377949.1 thermonuclease family protein -
  VW859_RS02005 (VW859_02005) - 392017..392283 (+) 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
  VW859_RS02010 (VW859_02010) - 392276..392551 (+) 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin -
  VW859_RS02015 (VW859_02015) - 392742..393386 (-) 645 WP_002377952.1 IS6 family transposase -
  VW859_RS02020 (VW859_02020) - 393519..394401 (+) 883 Protein_374 IS256 family transposase -
  VW859_RS02025 (VW859_02025) - 394706..395743 (-) 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
  VW859_RS02030 (VW859_02030) - 395769..396707 (-) 939 WP_002370935.1 2-dehydropantoate 2-reductase -
  VW859_RS02035 (VW859_02035) - 396835..397104 (-) 270 Protein_377 LPXTG cell wall anchor domain-containing protein -
  VW859_RS02040 (VW859_02040) - 397194..397379 (+) 186 WP_002358660.1 hypothetical protein -
  VW859_RS02045 (VW859_02045) - 397411..398019 (+) 609 Protein_379 IS6 family transposase -
  VW859_RS02050 (VW859_02050) bsh 398688..399662 (-) 975 WP_002355428.1 choloylglycine hydrolase -
  VW859_RS02055 (VW859_02055) - 399886..400530 (+) 645 WP_002377952.1 IS6 family transposase -
  VW859_RS02060 (VW859_02060) - 400703..401017 (+) 315 WP_002403043.1 hypothetical protein -
  VW859_RS02065 (VW859_02065) cylR2 401355..401555 (-) 201 WP_002370931.1 cytolysin regulator CylR2 -
  VW859_RS02070 (VW859_02070) cylL-L 401959..402165 (+) 207 WP_002358485.1 lanthipeptide cytolysin subunit CylL-L -
  VW859_RS02075 (VW859_02075) cylL-S 402199..402390 (+) 192 WP_002358181.1 lanthipeptide cytolysin subunit CylL-S -
  VW859_RS02080 (VW859_02080) - 402451..405432 (+) 2982 WP_002370929.1 type 2 lanthipeptide synthetase LanM family protein -
  VW859_RS02085 (VW859_02085) - 405444..407588 (+) 2145 WP_002370927.1 peptidase domain-containing ABC transporter -
  VW859_RS02090 (VW859_02090) - 407585..408823 (+) 1239 WP_002368283.1 S8 family serine peptidase -
  VW859_RS02095 (VW859_02095) - 408896..409807 (+) 912 WP_227264185.1 site-2 protease family protein -
  VW859_RS02100 (VW859_02100) - 410160..410723 (+) 564 WP_002370925.1 hypothetical protein -
  VW859_RS02105 (VW859_02105) - 411160..411627 (+) 468 WP_002370921.1 hypothetical protein -
  VW859_RS02110 (VW859_02110) - 411705..412286 (+) 582 WP_002401490.1 hypothetical protein -
  VW859_RS02115 (VW859_02115) - 412381..412587 (+) 207 WP_229017897.1 S41 family peptidase -
  VW859_RS02120 (VW859_02120) amaP 412857..413423 (+) 567 WP_010706722.1 alkaline shock response membrane anchor protein AmaP -
  VW859_RS02125 (VW859_02125) - 413440..413631 (+) 192 WP_002358517.1 DUF2273 domain-containing protein -
  VW859_RS02130 (VW859_02130) - 413660..414196 (+) 537 WP_002358518.1 Asp23/Gls24 family envelope stress response protein -
  VW859_RS02135 (VW859_02135) - 414471..420092 (+) 5622 WP_108095647.1 Rib/alpha-like domain-containing protein -
  VW859_RS02140 (VW859_02140) - 420292..421500 (+) 1209 WP_002358521.1 YSIRK-targeted surface antigen transcriptional regulator -
  VW859_RS02145 (VW859_02145) - 421630..422046 (+) 417 WP_002358522.1 hypothetical protein -
  VW859_RS02150 (VW859_02150) - 422182..422283 (-) 102 Protein_400 IS30 family transposase -
  VW859_RS02155 (VW859_02155) - 422271..423008 (+) 738 WP_002401428.1 hypothetical protein -
  VW859_RS02160 (VW859_02160) - 423054..423674 (-) 621 WP_033591050.1 recombinase family protein -
  VW859_RS02165 (VW859_02165) - 423858..424477 (+) 620 Protein_403 recombinase family protein -
  VW859_RS02170 (VW859_02170) - 424616..424768 (+) 153 WP_229204429.1 hypothetical protein -
  VW859_RS02175 (VW859_02175) - 425224..426042 (-) 819 WP_002358531.1 MurR/RpiR family transcriptional regulator -
  VW859_RS02180 (VW859_02180) - 426198..426905 (+) 708 WP_002358533.1 N-acetylmannosamine-6-phosphate 2-epimerase -
  VW859_RS02185 (VW859_02185) - 426898..428400 (+) 1503 WP_002358534.1 PTS transporter subunit EIIC -
  VW859_RS02190 (VW859_02190) - 428411..428893 (+) 483 WP_002358535.1 PTS glucose transporter subunit IIA -

Sequence


Protein


Download         Length: 152 a.a.        Molecular weight: 17179.08 Da        Isoelectric Point: 4.6511

>NTDB_id=926875 VW859_RS01945 WP_002365911.1 379344..379802(+) (ssb) [Enterococcus faecalis strain KS2H-124]
MINNVTLVGRLTKDPDLRYTQSGTAVGQFTLAINRNFTNANNEREADFINCVIWRKAAESLANYATKGTLIGLTGRIQTR
NYENQQGQRIYVTEVVTESFQLLESREVNEQRKEQATGKATFDKQSMDKPDPLDPFSPENSIVDISDNDLPF

Nucleotide


Download         Length: 459 bp        

>NTDB_id=926875 VW859_RS01945 WP_002365911.1 379344..379802(+) (ssb) [Enterococcus faecalis strain KS2H-124]
TTGATTAATAACGTTACATTAGTTGGACGATTAACCAAAGACCCAGATTTAAGGTATACGCAAAGTGGAACAGCCGTAGG
TCAATTTACGTTGGCCATTAATCGCAACTTTACCAATGCTAACAATGAAAGGGAAGCAGATTTTATCAACTGTGTTATTT
GGCGGAAAGCTGCAGAGTCATTAGCAAATTATGCAACAAAAGGGACTCTGATCGGTTTAACTGGTCGCATTCAAACAAGA
AACTATGAGAATCAACAAGGCCAGCGTATTTATGTAACTGAGGTTGTCACAGAAAGCTTCCAACTATTAGAATCAAGAGA
AGTAAACGAGCAACGAAAAGAACAGGCTACAGGTAAAGCTACGTTTGATAAACAGTCAATGGATAAACCTGATCCTCTGG
ATCCATTTTCGCCAGAAAATAGCATAGTGGATATTTCTGATAATGACCTGCCGTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB Q8VT46

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56.471

100

0.632

  ssbA Bacillus subtilis subsp. subtilis str. 168

47.283

100

0.572

  ssbB Bacillus subtilis subsp. subtilis str. 168

56.604

69.737

0.395

  ssbB/cilA Streptococcus mitis NCTC 12261

50

76.316

0.382

  ssbB Streptococcus sobrinus strain NIDR 6715-7

53.774

69.737

0.375

  ssbB/cilA Streptococcus pneumoniae TIGR4

49.138

76.316

0.375

  ssbB/cilA Streptococcus pneumoniae Rx1

48.276

76.316

0.368

  ssbB/cilA Streptococcus pneumoniae D39

48.276

76.316

0.368

  ssbB/cilA Streptococcus pneumoniae R6

48.276

76.316

0.368

  ssbB/cilA Streptococcus mitis SK321

48.276

76.316

0.368