Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VP459_RS13450 Genome accession   NZ_CP142600
Coordinates   2747350..2748324 (+) Length   324 a.a.
NCBI ID   WP_000697215.1    Uniprot ID   A0AAE8P9U4
Organism   Staphylococcus aureus strain M19060305     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2742350..2753324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VP459_RS13415 (VP459_13420) rpmG 2742810..2742959 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  VP459_RS13420 (VP459_13425) - 2743168..2743707 (+) 540 WP_000164551.1 5-formyltetrahydrofolate cyclo-ligase -
  VP459_RS13425 (VP459_13430) - 2743719..2745182 (+) 1464 WP_001017872.1 rhomboid family intramembrane serine protease -
  VP459_RS13430 (VP459_13435) - 2745163..2745366 (+) 204 WP_000087561.1 YqgQ family protein -
  VP459_RS13435 (VP459_13440) - 2745363..2746349 (+) 987 WP_000161315.1 ROK family glucokinase -
  VP459_RS13440 (VP459_13445) - 2746349..2746678 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  VP459_RS13445 (VP459_13450) - 2746675..2747298 (+) 624 WP_001223006.1 MBL fold metallo-hydrolase -
  VP459_RS13450 (VP459_13455) comGA 2747350..2748324 (+) 975 WP_000697215.1 competence type IV pilus ATPase ComGA Machinery gene
  VP459_RS13455 (VP459_13460) comGB 2748296..2749366 (+) 1071 WP_000776403.1 competence type IV pilus assembly protein ComGB Machinery gene
  VP459_RS13460 (VP459_13465) comGC 2749380..2749691 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  VP459_RS13465 (VP459_13470) comGD 2749669..2750115 (+) 447 WP_075583651.1 competence type IV pilus minor pilin ComGD Machinery gene
  VP459_RS13470 (VP459_13475) comGE 2750102..2750401 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  VP459_RS13475 (VP459_13480) comGF 2750319..2750816 (+) 498 WP_029697681.1 competence type IV pilus minor pilin ComGF Machinery gene
  VP459_RS13480 (VP459_13485) - 2750913..2751059 (+) 147 WP_001789879.1 hypothetical protein -
  VP459_RS13485 (VP459_13490) - 2751049..2751573 (+) 525 WP_001015122.1 shikimate kinase -
  VP459_RS13490 (VP459_13495) gcvT 2751732..2752823 (+) 1092 WP_000093355.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36853.90 Da        Isoelectric Point: 8.8516

>NTDB_id=926162 VP459_RS13450 WP_000697215.1 2747350..2748324(+) (comGA) [Staphylococcus aureus strain M19060305]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNGIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGVSVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=926162 VP459_RS13450 WP_000697215.1 2747350..2748324(+) (comGA) [Staphylococcus aureus strain M19060305]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATGGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATTTATTTTTTGAGAATATCAACATTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAACAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTTCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCCGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGTGTTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.383

100

0.994
  comGA Staphylococcus aureus N315

99.383

100

0.994