Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCU50_RS11700 Genome accession   NZ_AP025514
Coordinates   2617856..2619541 (+) Length   561 a.a.
NCBI ID   WP_060468583.1    Uniprot ID   A0A109D820
Organism   Vibrio toranzoniae strain CECT 7225     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2612856..2624541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU50_RS11680 pdhR 2614369..2615130 (-) 762 WP_060468580.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  OCU50_RS11685 ampD 2615612..2616212 (-) 601 Protein_2243 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCU50_RS11690 nadC 2616296..2617186 (+) 891 WP_060468581.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCU50_RS11695 pilA 2617443..2617853 (+) 411 WP_060468582.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  OCU50_RS11700 pilB 2617856..2619541 (+) 1686 WP_060468583.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCU50_RS11705 pilC 2619557..2620786 (+) 1230 WP_060468584.1 type II secretion system F family protein Machinery gene
  OCU50_RS11710 pilD 2620854..2621723 (+) 870 WP_060468585.1 A24 family peptidase Machinery gene
  OCU50_RS11715 coaE 2621726..2622340 (+) 615 WP_060468586.1 dephospho-CoA kinase -
  OCU50_RS11720 zapD 2622367..2623107 (+) 741 WP_029627015.1 cell division protein ZapD -
  OCU50_RS11725 yacG 2623188..2623385 (+) 198 WP_017056605.1 DNA gyrase inhibitor YacG -
  OCU50_RS11730 rplS 2623579..2623932 (-) 354 WP_046223309.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62232.10 Da        Isoelectric Point: 5.2009

>NTDB_id=92408 OCU50_RS11700 WP_060468583.1 2617856..2619541(+) (pilB) [Vibrio toranzoniae strain CECT 7225]
MLTNLPTVLRQANLLSLTQEQAVAEHVQASGVSTPEALLVLDLFSGYSLASNIKTIFGLPLVQLANTNYETLCEQLGLRE
LITKYCAIPIAVSSSTLTLASADPTDLHAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSIFGQDSKRKEITQDELAN
LVEVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEEQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLRGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTVDDSPDIFLRHSIPNSQT
IYKANPQGCNECTQGYSGRVGIYEVMPFTDQLKNSLINKPNALTIESLARREGMRTLQESGLDKLLEGITSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=92408 OCU50_RS11700 WP_060468583.1 2617856..2619541(+) (pilB) [Vibrio toranzoniae strain CECT 7225]
ATGCTCACTAATCTCCCAACCGTTCTACGTCAGGCTAATCTACTTAGTCTGACTCAAGAACAAGCTGTTGCGGAACATGT
GCAAGCTTCAGGTGTTTCTACGCCTGAAGCTTTACTCGTTCTGGATTTATTCAGCGGCTATTCCTTAGCAAGCAATATCA
AAACCATTTTCGGTTTACCGTTAGTACAACTCGCCAATACCAACTACGAAACCTTATGTGAACAGCTAGGGCTTCGTGAG
CTGATCACCAAGTATTGTGCTATTCCGATTGCCGTTTCTAGTTCTACGCTCACGCTCGCCTCTGCCGACCCGACCGACTT
ACACGCTGAAGACGATTTCCGATTTGCTACAGGGTTGCAAATTGAACTCGTCGTCGCTAATTATTCAGAATTAGAAGGCG
CGATACGCAAACTGTATGGCCGTTCGATTTTCGGACAAGACTCCAAGCGAAAAGAGATCACTCAAGATGAACTCGCCAAC
CTAGTTGAAGTCTCTGACGATGAAATCACGTCAATTGAAGATCTCAGCCAAGATGATTCCCCTGTGAGCCGTTTTATTAA
TCAAATATTGGTTGATGCGGTGCGCAAAGGCGCCTCTGATATCCACTTCGAACCTTATGAAGAAAACTACCGAGTTCGCC
TGCGTTGCGATGGCATCCTTGTCGAAATTCAACAGCCCGCTTCTCATTTAAGTCGTCGGTTATCGGCCCGCTTAAAAATT
CTCGCTAAGTTGGATATTGCCGAGCGTCGATTGCCTCAAGACGGCCGCATCAAGTTGCGGTTAAATGATGAGCTCGCGAT
TGATATGCGTGTATCAACACTGCCAACATTATGGGGCGAAAAGATCGTACTGCGTCTGCTAGACAGTAGCGCTGCCAATT
TAGATATCGATAAACTCGGCTACAGCGAAGAACAAAAAGCACTCTACCTTAATGCACTGAAACGACCGCAAGGGATGATT
TTAATGACCGGGCCAACCGGCAGTGGCAAAACAGTCTCTCTTTACACCGGCCTTCGAGTTCTTAACACCACAGAACGCAA
CATCTCTACCGCAGAAGATCCGGTCGAAATTAACTTACGTGGTATCAACCAAGTACAAGTCACACCAAAGATCGGCTTTG
GATTTGCTGAAGCACTACGATCCTTTTTACGACAAGACCCAGACATCGTGATGGTCGGAGAGATCCGTGACTTAGAAACG
GCAGAGATAGCGATCAAAGCCTCGCAAACAGGCCACTTAGTCCTTTCAACACTGCATACAAACTCTGCCGCAGAAACCGT
CACTCGACTCGCTCACATGGGGATAGAGCCCTTTAACCTTGCTTCATCATTAAGCCTAATTATTGCTCAGCGACTCGCAA
GGCGCTTGTGCAACCATTGCAAAACGGTGGATGATTCACCGGACATATTTCTGCGTCACTCTATTCCCAACAGCCAAACC
ATTTACAAGGCCAACCCACAAGGGTGCAATGAGTGTACTCAAGGGTATTCTGGCCGAGTCGGTATCTACGAAGTGATGCC
GTTTACCGACCAACTCAAAAACAGCCTTATCAACAAGCCTAACGCGCTAACAATTGAAAGCCTTGCTCGTCGGGAAGGCA
TGCGAACCCTACAAGAATCAGGGCTTGATAAGCTGCTTGAAGGTATCACCAGCTATCAAGAACTACAGCGTGTTCTGTAC
ATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A109D820

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.582

100

0.74
  pilB Vibrio campbellii strain DS40M4

70.463

100

0.706
  pilB Vibrio parahaemolyticus RIMD 2210633

70.107

100

0.702
  pilB Acinetobacter baumannii D1279779

51.06

92.513

0.472
  pilB Legionella pneumophila strain ERS1305867

50.388

91.979

0.463
  pilB Acinetobacter baylyi ADP1

49.14

93.226

0.458
  pilF Neisseria gonorrhoeae MS11

44.144

98.93

0.437
  pilB/pilB1 Synechocystis sp. PCC 6803

35.811

100

0.378
  pilF Thermus thermophilus HB27

39.231

92.692

0.364

Multiple sequence alignment