Detailed information NTG0092410

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OCU50_RS11710
• Genome accession: NZ_AP025514
• Coordinates: 2620854..2621723 (+)
• Length: 289 a.a.
• NCBI ID: WP_060468585.1
• Uniprot ID: A0A109D829
• Organism: Vibrio toranzoniae strain CECT 7225
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2615854..2626723

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCU50_RS11690	nadC	2616296..2617186 (+)	891	WP_060468581.1	carboxylating nicotinate-nucleotide diphosphorylase	-
OCU50_RS11695	pilA	2617443..2617853 (+)	411	WP_060468582.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
OCU50_RS11700	pilB	2617856..2619541 (+)	1686	WP_060468583.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OCU50_RS11705	pilC	2619557..2620786 (+)	1230	WP_060468584.1	type II secretion system F family protein	Machinery gene
OCU50_RS11710	pilD	2620854..2621723 (+)	870	WP_060468585.1	A24 family peptidase	Machinery gene
OCU50_RS11715	coaE	2621726..2622340 (+)	615	WP_060468586.1	dephospho-CoA kinase	-
OCU50_RS11720	zapD	2622367..2623107 (+)	741	WP_029627015.1	cell division protein ZapD	-
OCU50_RS11725	yacG	2623188..2623385 (+)	198	WP_017056605.1	DNA gyrase inhibitor YacG	-
OCU50_RS11730	rplS	2623579..2623932 (-)	354	WP_046223309.1	50S ribosomal protein L19	-
OCU50_RS11735	trmD	2623992..2624732 (-)	741	WP_017629426.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
OCU50_RS11740	rimM	2624759..2625313 (-)	555	WP_017056602.1	ribosome maturation factor RimM	-
OCU50_RS11745	rpsP	2625338..2625586 (-)	249	WP_004735508.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32555.62 Da
• Isoelectric Point: 5.9782

>NTDB_id=92410 OCU50_RS11710 WP_060468585.1 2620854..2621723(+) (pilD) [Vibrio toranzoniae strain CECT 7225]
MEVLHYYPWLFPVLAFIFSLLIGSFLNVVIHRLPIMMEREWQQECSEYFSQYKIPAPEGEFNLSTPRSTCPKCDTQLRVI
DNIPVLSWLFLKGKCHSCANPISARYPLVELLTAILCTVVASHFGFSFYAIALIFFTFALITATFIDLDTMLLPDQITLP
LVWSGIALALFNISPVSLQDSVVGAMAGYLALWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQHLPMIILLSSLVGL
IFGLIQLRLKQQGIDKAFPFGPYLAIAGWVSLMWGNDIMDWYFTSVLGI

Nucleotide

• Length: 870 bp

>NTDB_id=92410 OCU50_RS11710 WP_060468585.1 2620854..2621723(+) (pilD) [Vibrio toranzoniae strain CECT 7225]
ATGGAAGTACTTCACTACTATCCTTGGCTATTCCCTGTATTAGCTTTCATTTTTAGCCTTCTTATCGGCAGCTTTCTCAA
CGTTGTAATTCACCGATTACCGATTATGATGGAGCGTGAGTGGCAACAAGAGTGCTCCGAATATTTCTCTCAATATAAAA
TTCCAGCACCTGAAGGGGAATTTAATCTTAGCACTCCACGTTCGACTTGCCCGAAATGTGACACCCAATTAAGAGTCATC
GACAATATCCCCGTTTTGAGCTGGTTGTTCTTGAAGGGCAAGTGCCACAGTTGTGCGAATCCAATCAGTGCTCGCTACCC
TTTGGTAGAATTGCTTACAGCCATACTTTGTACCGTGGTGGCGAGCCACTTTGGTTTTAGTTTCTACGCTATTGCGCTGA
TCTTTTTCACCTTCGCATTGATTACAGCGACCTTTATCGATCTCGATACTATGCTGCTGCCTGATCAGATCACCTTACCT
TTGGTTTGGTCTGGTATTGCATTAGCTCTGTTTAATATCAGCCCAGTATCTCTTCAGGATTCGGTGGTTGGTGCAATGGC
AGGCTACCTAGCTTTATGGTCAGTGTACTGGTTGTTTAAGCTGCTAACTGGCAAAGAAGGAATGGGCTATGGTGACTTTA
AATTATTAGCAGCACTTGGGGCATGGCTTGGGTGGCAGCACCTACCAATGATCATTCTTTTATCATCACTCGTCGGCCTT
ATTTTTGGATTGATTCAACTGAGGTTAAAACAACAAGGCATAGATAAAGCTTTCCCCTTTGGACCTTACCTCGCCATTGC
TGGTTGGGTAAGCTTGATGTGGGGTAACGACATCATGGATTGGTATTTCACGTCTGTATTAGGAATTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A109D829

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	73.702	100	0.737
pilD	Vibrio cholerae strain A1552	69.444	99.654	0.692
pilD	Acinetobacter baumannii D1279779	51.311	92.388	0.474
pilD	Acinetobacter nosocomialis M2	51.311	92.388	0.474
pilD	Neisseria gonorrhoeae MS11	46.014	95.502	0.439