Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VPN06_RS06210 Genome accession   NZ_CP141950
Coordinates   1295488..1296885 (-) Length   465 a.a.
NCBI ID   WP_015432350.1    Uniprot ID   A0A179D0F8
Organism   Bibersteinia trehalosi strain M01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1290488..1301885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VPN06_RS06180 - 1290848..1292032 (+) 1185 WP_324681445.1 FAD-dependent monooxygenase -
  VPN06_RS06185 - 1292167..1292721 (+) 555 WP_025267130.1 hypothetical protein -
  VPN06_RS06190 yacG 1292781..1292987 (-) 207 WP_015432346.1 DNA gyrase inhibitor YacG -
  VPN06_RS06195 coaE 1292984..1293607 (-) 624 WP_324681449.1 dephospho-CoA kinase -
  VPN06_RS06200 - 1293637..1294233 (-) 597 WP_324681451.1 A24 family peptidase -
  VPN06_RS06205 pilC 1294299..1295495 (-) 1197 WP_324683794.1 type II secretion system F family protein Machinery gene
  VPN06_RS06210 pilB 1295488..1296885 (-) 1398 WP_015432350.1 GspE/PulE family protein Machinery gene
  VPN06_RS06215 pilA 1296943..1297389 (-) 447 WP_025267127.1 prepilin peptidase-dependent pilin Machinery gene
  VPN06_RS06220 radA 1297564..1298943 (+) 1380 WP_324681454.1 DNA repair protein RadA Machinery gene
  VPN06_RS06225 - 1299035..1300579 (-) 1545 WP_025289241.1 Na+/H+ antiporter NhaC family protein -
  VPN06_RS06230 - 1301084..1301485 (+) 402 WP_324681457.1 H-NS family nucleoid-associated regulatory protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53408.14 Da        Isoelectric Point: 5.3553

>NTDB_id=920601 VPN06_RS06210 WP_015432350.1 1295488..1296885(-) (pilB) [Bibersteinia trehalosi strain M01]
MSKYTICALNSSTQFEISERQWQKNCDEKQLLRRYLAMPLQENEQKIWLALDDENNLNACEIFTFLTHKQVEPVLVESEE
LKFLLNQLSPENEVAIDFDEFQTLHIDEQTFSDGLDFNDPIIRTLDNLFKFCLQKNASDIHIEPHKQHLMIRLRIDGVLQ
LYKTLPTHFSSRLLSRLKLLAKLDISENRLPQDGQFSFTTELNEILDFRVSTLPVQYGEKMVLRLQKNKPTHLDFLSLGF
TQAQQEKLLNALKQPQGMILVTGPTGSGKSITLYSALNYLNQSDKHILTAEDPIEIEIEGLIQTQVNHAIQLDFSRLLRT
FLRQDPDIIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAIERLNQLGIKDYEIKNSLLLVIAQRLVRLICPKCK
GEGCQECYQGYKGRIGVFQLFSRVAEQFEKSTACLDYPTLQESAKHKLAENLTDLHEIERVFGHV

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=920601 VPN06_RS06210 WP_015432350.1 1295488..1296885(-) (pilB) [Bibersteinia trehalosi strain M01]
ATGTCTAAATACACCATCTGCGCACTCAACTCATCAACCCAGTTTGAAATCAGTGAACGACAATGGCAGAAAAACTGCGA
TGAAAAGCAACTACTACGCCGTTATCTTGCTATGCCTCTACAGGAAAATGAGCAGAAAATCTGGCTGGCGCTTGATGATG
AAAATAATCTAAATGCTTGTGAAATTTTTACTTTTCTCACGCATAAACAGGTTGAACCTGTATTAGTTGAAAGCGAGGAA
TTGAAATTTTTACTTAATCAGTTATCCCCTGAAAATGAGGTAGCAATAGACTTTGATGAATTTCAAACTCTACATATCGA
TGAGCAAACATTCTCTGACGGACTTGATTTTAATGATCCGATTATTCGCACTCTAGATAACCTATTTAAATTCTGTTTAC
AGAAAAATGCTTCTGATATTCACATTGAGCCCCATAAACAGCATTTAATGATTCGTCTGCGTATTGATGGTGTACTCCAA
CTTTATAAAACCTTACCCACCCATTTTTCAAGTCGTTTACTTTCTCGTCTGAAACTATTAGCCAAACTAGATATCAGTGA
AAATCGTTTACCACAAGATGGACAGTTTAGCTTTACTACCGAACTGAATGAAATCTTAGATTTTCGTGTTTCTACCCTAC
CTGTGCAATATGGTGAAAAAATGGTTCTGCGCTTACAAAAAAATAAACCTACTCACTTGGATTTTCTCTCGCTCGGTTTT
ACCCAAGCGCAACAAGAGAAACTGCTCAATGCTTTAAAACAACCTCAAGGAATGATTCTAGTAACAGGCCCAACTGGAAG
TGGTAAAAGTATTACGCTCTATAGTGCATTGAATTATTTAAACCAAAGCGATAAGCATATTCTTACTGCTGAAGATCCTA
TTGAAATTGAGATTGAAGGCTTAATTCAAACGCAAGTAAACCACGCTATTCAACTTGATTTTAGCAGGCTATTACGTACA
TTTCTCCGCCAAGATCCAGATATTATTATGCTGGGAGAAATTCGAGATGAAGAGAGCGCCGAAATTGCATTACGAGCAGC
ACAAACAGGCCATCTAGTGCTTTCTACTTTACATACAAATGATGCGCCCTCAGCGATTGAACGCCTAAATCAACTCGGTA
TTAAAGACTATGAAATAAAAAATTCACTGTTATTAGTGATTGCTCAACGTTTGGTTCGCCTTATTTGCCCTAAATGCAAG
GGCGAAGGTTGCCAAGAATGTTATCAAGGCTACAAAGGACGAATAGGCGTATTTCAACTTTTCTCTCGTGTTGCAGAACA
GTTTGAAAAATCAACCGCTTGCTTAGACTATCCCACATTACAGGAAAGCGCAAAGCACAAATTAGCTGAAAATCTAACCG
ACTTGCATGAAATTGAACGGGTATTTGGCCATGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(133-393)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-91)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A179D0F8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

70.69

99.785

0.705
  pilB Haemophilus influenzae 86-028NP

57.363

97.849

0.561
  pilB Haemophilus influenzae Rd KW20

57.143

97.849

0.559
  pilB Vibrio cholerae strain A1552

40.871

100

0.424
  pilB Vibrio parahaemolyticus RIMD 2210633

39.2

100

0.422
  pilB Vibrio campbellii strain DS40M4

39.709

100

0.411
  pilB Legionella pneumophila strain ERS1305867

37.602

100

0.398
  pilB Acinetobacter baumannii D1279779

37.398

100

0.396
  pilB Acinetobacter baylyi ADP1

37.5

100

0.394
  pilF Thermus thermophilus HB27

35.443

100

0.361