Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VPN06_RS06215 Genome accession   NZ_CP141950
Coordinates   1296943..1297389 (-) Length   148 a.a.
NCBI ID   WP_025267127.1    Uniprot ID   A0A4V7IAF9
Organism   Bibersteinia trehalosi strain M01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1291943..1302389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VPN06_RS06185 - 1292167..1292721 (+) 555 WP_025267130.1 hypothetical protein -
  VPN06_RS06190 yacG 1292781..1292987 (-) 207 WP_015432346.1 DNA gyrase inhibitor YacG -
  VPN06_RS06195 coaE 1292984..1293607 (-) 624 WP_324681449.1 dephospho-CoA kinase -
  VPN06_RS06200 - 1293637..1294233 (-) 597 WP_324681451.1 A24 family peptidase -
  VPN06_RS06205 pilC 1294299..1295495 (-) 1197 WP_324683794.1 type II secretion system F family protein Machinery gene
  VPN06_RS06210 pilB 1295488..1296885 (-) 1398 WP_015432350.1 GspE/PulE family protein Machinery gene
  VPN06_RS06215 pilA 1296943..1297389 (-) 447 WP_025267127.1 prepilin peptidase-dependent pilin Machinery gene
  VPN06_RS06220 radA 1297564..1298943 (+) 1380 WP_324681454.1 DNA repair protein RadA Machinery gene
  VPN06_RS06225 - 1299035..1300579 (-) 1545 WP_025289241.1 Na+/H+ antiporter NhaC family protein -
  VPN06_RS06230 - 1301084..1301485 (+) 402 WP_324681457.1 H-NS family nucleoid-associated regulatory protein -
  VPN06_RS06235 rnhA 1301827..1302285 (+) 459 WP_064318020.1 ribonuclease HI -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15806.99 Da        Isoelectric Point: 7.5807

>NTDB_id=920602 VPN06_RS06215 WP_025267127.1 1296943..1297389(-) (pilA) [Bibersteinia trehalosi strain M01]
MKISNFRPLQKGFTLIELMIVIAIIAILATIAIPSYTNYTQKAALSEMLRASASYKSDVEICIYNTGATTNCSAGSNGIQ
AAKTESSDTKYLKSVSVTNGIINVEGKGSLDGYSYTMTPKFANNNITWSVNCTGADTGLFPANFCSSE

Nucleotide


Download         Length: 447 bp        

>NTDB_id=920602 VPN06_RS06215 WP_025267127.1 1296943..1297389(-) (pilA) [Bibersteinia trehalosi strain M01]
ATGAAAATTTCTAACTTTCGCCCACTTCAAAAGGGCTTTACTTTAATTGAACTCATGATCGTGATTGCGATCATTGCAAT
TCTCGCTACTATCGCGATTCCATCTTATACCAACTATACTCAAAAAGCGGCTCTCTCTGAAATGTTACGTGCTTCTGCCT
CTTACAAATCAGATGTAGAAATTTGTATTTATAACACAGGGGCTACTACCAACTGCTCCGCAGGCTCAAATGGAATTCAA
GCTGCGAAAACAGAGAGTAGTGATACAAAATATTTGAAATCAGTCTCTGTCACTAATGGCATTATTAATGTTGAAGGTAA
AGGCAGCCTTGATGGATATAGCTATACCATGACACCTAAATTTGCTAACAACAATATTACTTGGTCAGTCAATTGCACTG
GGGCAGACACAGGCTTATTCCCTGCTAATTTCTGTAGTTCGGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V7IAF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

71.141

100

0.716
  pilA Haemophilus influenzae 86-028NP

57.432

100

0.574
  pilA Haemophilus influenzae Rd KW20

56.081

100

0.561
  comP Acinetobacter baylyi ADP1

44.094

85.811

0.378
  pilA Vibrio campbellii strain DS40M4

38.028

95.946

0.365