Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VER99_RS11600 Genome accession   NZ_CP141822
Coordinates   2579055..2579513 (+) Length   152 a.a.
NCBI ID   WP_020333947.1    Uniprot ID   A0AAN0Y3X9
Organism   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2574055..2584513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VER99_RS11585 (VER99_11585) pdhR 2576086..2576853 (-) 768 WP_014232947.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VER99_RS11590 (VER99_11590) ampD 2577263..2577808 (-) 546 WP_020333945.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VER99_RS11595 (VER99_11595) nadC 2577901..2578788 (+) 888 WP_020333946.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VER99_RS11600 (VER99_11600) pilA 2579055..2579513 (+) 459 WP_020333947.1 pilin Machinery gene
  VER99_RS11605 (VER99_11605) pilB 2579514..2581199 (+) 1686 WP_020333948.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VER99_RS11610 (VER99_11610) pilC 2581230..2582453 (+) 1224 WP_020333949.1 type II secretion system F family protein Machinery gene
  VER99_RS11615 (VER99_11615) pilD 2582521..2583390 (+) 870 WP_020333950.1 prepilin peptidase Machinery gene
  VER99_RS11620 (VER99_11620) coaE 2583391..2584005 (+) 615 WP_020333951.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15920.27 Da        Isoelectric Point: 7.7804

>NTDB_id=919703 VER99_RS11600 WP_020333947.1 2579055..2579513(+) (pilA) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MKSNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSALGVGLANITALKTNIEDYIATEGSFPSTTSGTKADFT
RLGTVEDMGDGNVLITPTSDGSLDGTVKFTFDAGVVNTQKIQLVRSGGLWICETTAASEISPKGCAAGSTIK

Nucleotide


Download         Length: 459 bp        

>NTDB_id=919703 VER99_RS11600 WP_020333947.1 2579055..2579513(+) (pilA) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
ATGAAGTCAAATAAACAGAAAAAACAGCAAGGCTTTACGCTGATTGAATTAATGATCGTGGTGGCGGTAATAGGGGTTTT
GGCAGCAATTGCTATCCCTCAGTACCAAAACTACGTAAAGAAATCAGCTTTAGGAGTAGGCCTAGCAAATATTACTGCTC
TGAAAACTAACATCGAGGATTATATTGCTACTGAAGGTAGCTTTCCTTCAACAACATCAGGTACCAAAGCTGATTTTACG
CGGCTCGGTACCGTAGAAGACATGGGAGATGGCAACGTTCTAATCACCCCTACAAGCGACGGCTCTTTAGATGGAACAGT
AAAATTCACCTTTGATGCAGGTGTCGTCAATACACAAAAGATTCAGTTAGTTCGATCTGGTGGACTTTGGATATGTGAAA
CGACAGCAGCAAGTGAAATATCTCCAAAAGGTTGTGCTGCTGGTTCAACGATTAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

77.632

100

0.776
  pilA Vibrio cholerae C6706

77.632

100

0.776
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

77.632

100

0.776
  pilA Pseudomonas aeruginosa PAK

43.226

100

0.441
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

97.368

0.414
  pilA Acinetobacter baumannii strain A118

40.397

99.342

0.401
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.86

94.079

0.375